danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2021-65-5-582-591dan-1008Research ArticleБИОЛОГИЯBIOLOGYОпределение возраста человека по образцам крови на основании анализа метилирования CpG-динуклеотидовDetermining the human chronological age from the blood samples on the basis of the analysis of CpG-dinucleotides methylationЛемешВ. А.LemeshV. A.

Лемеш Валентина Александровна – канд. биол. наук, доцент, заведующий лабораторией

ул. Академическая, 27, 220072, Минск

Lemesh Valentina A. – Ph. D. (Biology), Assistant professor, Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk

v.lemesh@igc.byКипеньВ. Н.KipenV. N.

Кипень Вячеслав Николаевич – канд. биол. наук, вед. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Kipen Viachaslau N. – Ph. D. (Biology), Leading Researcher

27, Akademicheskaya Str., 220072, Minsk

v.kipen@igc.byБогдановаМ. В.BahdanavaM. V.

Богданова Марина Владимировна – канд. биол. наук, вед. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Bahdanava Marina V. – Ph. D. (Biology), Leading researcher

27, Akademicheskaya Str., 220072, Minsk

M.Bogdanova@igc.byБураковаА. А.BurakovaA. A.

Буракова Арина Александровна – мл. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Burakova Arina A. – Junior researcher

27, Akademicheskaya Str., 220072, Minsk

arina.burakova@mail.ruБулгакА. Г.BulgakA. A.

Булгак Александр Григорьевич – член-корреспондент НАН Беларуси, д-р мед. наук, профессор, гл. науч. сотрудник

ул. Розы Люксембург, 110Б, 220036, Минск

Bulgak Alexander G. – Corresponding Member, D. Sc.(Medicine), Professor, Chief researcher

110B, Roza Lyuksemburg Str., 220036, Minsk

БайдаА. В.BaydaA. V.

Байда Александр Васильевич – канд. мед. наук, заведующий кафедрой

ул. Петруся Бровки, 3, 220013, Минск

Bayda Alexander V. – Ph. D. (Medicine), Head of the Laboratory

3, P. Brovka Str., 220013, Minsk

alvab@tut.byЗотоваО. В.ZotovaV. V.

Зотова Ольга Владимировна – канд. мед. наук, врач-кардиолог, научный сотрудник

ул. Розы Люксембург, 110Б, 220036, Минск

Zotova Volga V. – Ph. D. (Medicine), Cardiologist, Researcher

110B, Roza Lyuksemburg Str., 220036, Minsk

doxtar@tut.byДобышО. И.DobyshV. I.

Добыш Ольга Игоревна – мл. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Dobysh Volga I. – Junior researcher

27, Akademicheskaya Str., 220072, Minsk

olyadobysh@gmail.comул. Академическая, 27, 220072, МинскБеларусьInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusBelarusРеспубликанский научно-практический центр «Кардиология»БеларусьScientific and Practical Centre «Cardiology»BelarusБелорусская медицинская академия последипломного образованияБеларусьБелорусская медицинская академия последипломного образованияBelarusInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusBelarus202107112021655582–591Copyright © Лемеш В.А., Кипень В.Н., Богданова М.В., Буракова А.А., Булгак А.Г., Байда А.В., Зотова О.В., Добыш О.И., 20212021Лемеш В.А., Кипень В.Н., Богданова М.В., Буракова А.А., Булгак А.Г., Байда А.В., Зотова О.В., Добыш О.И.Lemesh V.A., Kipen V.N., Bahdanava M.V., Burakova A.A., Bulgak A.A., Bayda A.V., Zotova V.V., Dobysh V.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1008

На основании биоинформатического и статистического анализа GEO-проектов по определению полногеномного профиля метилирования ДНК человека сформирован перечень из 27 CpG-динуклеотидов с высоким прогностическим потенциалом для создания моделей предсказания возраста человека по образцам крови. Определен уровень метилирования для 245 образцов индивидов из Республики Беларусь, рассчитаны коэффициенты корреляции R и построены математические модели определения возраста человека. Среднее значение точности предсказания возраста по образцам крови с использованием 12 CpG-динуклеотидов составило 3,4 года (для мужчин – 3,3, для женщин – 3,5). Полученные результаты будут положены в основу разработки калькуляторов предсказания возраста индивида по биологическим следам для экспертов-криминалистов.

Based on the bioinformatic and statistical analysis of the GEO-projects to determine the genome-wide profile of human DNA methylation, a list of 27 CpG dinucleotides with a high predictive potential was formed to create models for prediction of the human age from blood samples. The methylation level was determined for 245 samples of individuals from the Republic of Belarus. The correlation coefficients R were calculated, and the mathematical models for determining the age of an individual were constructed. The average accuracy value of the age prediction from blood samples using 12 CpG-dinucleotides was 3.4 years (for men – 3.3, for women – 3.5). The results obtained will be used as a basis for development of calculators for predicting the age of an individual based on the biological traces for forensic experts.

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The authors declare that there are no conflicts of interest present.