danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2022-66-1-43-54dan-1037Research ArticleХИМИЯCHEMISTRYНекоторые металлсвязывающие свойства рекомбинантного лактоферрина человека из молока трансгенных козSome metal binding properties of recombinant human lactoferrin from the milk of transgenic goatsСеменовД. А.SemenovD. A.

Семенов Дмитрий Александрович – научный сотрудник.

Ул. Купревича, 5/2, 220141, Минск

Semenov Dmitry A. – Researcher.

5/2, Kuprevich Str., 220141, Minsk

dsemenov@iboch.byКуприенкоО. С.KuprienkoO. S.

Куприенко Ольга Сергеевна – кандидат химических наук, старший научный сотрудник.

Ул. Купревича, 5/2, 220141, Минск

Kuprienko Olga S. – Ph. D. (Chemistry), Senior Researcher.

5/2, Kuprevich Str., 220141, Minsk

kuprienko@iboch.byВашкевичИ. И.VashkevichI. I.

Вашкевич Ирина Игнатьевна – кандидат химических наук, ведущий научный сотрудник.

Ул. Купревича, 5/2, 220141, Минск

Vashkevich Irina I. – Ph. D. (Chemistry), Leading Researcher.

5/2, Kuprevich Str., 220141, Minsk

vashkevich@iboch.byСвиридовО. В.SviridovO. V.

Свиридов Олег Васильевич – доктор химических наук, заведующий лабораторией.

Ул. Купревича, 5/2, 220141, Минск

Sviridov Oleg V. – D. Sc. (Chemistry), Head of the Laboratory.

5/2, Kuprevich Str., 220141, Minsk

sviridov@iboch.byInstitute of Bioorganic Chemistry, National Academy of Sciences of BelarusInstitute of Bioorganic Chemistry, National Academy of Sciences of Belarus2022090320226614354Copyright © Семенов Д.А., Куприенко О.С., Вашкевич И.И., Свиридов О.В., 20222022Семенов Д.А., Куприенко О.С., Вашкевич И.И., Свиридов О.В.Semenov D.A., Kuprienko O.S., Vashkevich I.I., Sviridov O.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1037

Методами спектрофотометрии, флуоресцентной спектроскопии и масс-спектрометрии показано, что человеческий рекомбинантный лактоферрин (рчЛФ), выделенный из молока трансгенных коз, способен связывать ионы железа и европия. Получены апо-форма рчЛФ, а также комплексы этого белка с Fe3+ и Eu3+, в которых степень насыщения рчЛФ металлом соответственно составила 76 и 22 %. Предложен способ регистрации общего или высвободившегося лантанида в системе, содержащей комплекс рчЛФ–Eu3+, при кислом или нейтральном значении рН и высоких или низких концентрациях хелатирующих агентов по интенсивности времяразрешенной флуоресценции.

In this research, the ability of pure recombinant human lactoferrin (rhLF), originated from the milk of transgenic goats, to bind ferric and europium ions has been shown by the methods of spectrophotometry, fluorescent spectroscopy, and inductively coupled plasma mass spectrometry. The apo-form of rhLF and its complexes with Fe3+ or Eu3+ saturated in 76 or 22 %, respectively, were obtained. A method for detection of total or released (“free”) lanthanide at acidic or neutral pH and high or low concentrations of chelating agents by time-resolved fluorescence was proposed.

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The authors declare that there are no conflicts of interest present.