danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2022-66-3-321-329dan-1070Research ArticleБИОЛОГИЯBIOLOGYАнализ антимикробных метаболитов бактерий Bacillus amyloliquefaciens БИМ B-1125 – основы биопрепарата Бакто-хелсAnalysis of antimicrobial metabolites of bacteria Bacillus amyloliquefaciens BIM B-1125 – the basis of Baсto-health biological preparationКанторК. В.KantorK. V.

Кантор Карина Викторовна – науч. сотрудник

ул. Купревича, 2, 220141, Минск

Kantor Karina V. – Researcher

2, Kuprevich Str., 220141, Minsk

kantorkarina@rambler.ruПроскурнинаИ. А.ProskurninaI. A.

Проскурнина Ирина Александровна – заведующий сектором

ул. Купревича, 2, 220141, Минск

Proskurnina Irina A. – Head of the Sector

2, Kuprevich Str., 220141, Minsk

irina_pros@tut.byСверчковаН. В.SverchkovaN. V.

Сверчкова Наталья Владимировна – канд. биол. наук, вед. науч. сотрудник

ул. Купревича, 2, 220141, Минск

Sverchkova Natalia V. – Ph. D. (Biology), Leading Researcher.

2, Kuprevich Str., 220141, Minsk

sverchkova@mbio.bas-net.byКоломиецЭ. И.KolomietsE. I.

Коломиец Эмилия Ивановна – академик, д-р биол. наук, профессор, гл. науч. сотрудник

ул. Купревича, 2, 220141, Минск

Kolomiets Emilia I. – Academician, D. Sc. (Biology), Professor, Chief Researcher

2, Kuprevich Str., 220141, Minsk

kolomiets@mbio.bas-net.byИнститут микробиологии НАН БеларусиInstitute of Microbiology of the National Academy of Sciences of Belarus202201072022663321329Copyright © Кантор К.В., Проскурнина И.А., Сверчкова Н.В., Коломиец Э.И., 20222022Кантор К.В., Проскурнина И.А., Сверчкова Н.В., Коломиец Э.И.Kantor K.V., Proskurnina I.A., Sverchkova N.V., Kolomiets E.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1070

Выделены и охарактеризованы антимикробные метаболиты штамма бактерий B. amyloliquefaciens БИМ В-1125 – основы пробиотического препарата для профилактики и лечения болезней рыб ценных видов. Установлена экстрацеллюлярная локализация антимикробных соединений; показана их устойчивость в диапазоне температур 50–100 °С и в диапазоне рН 2–10. С помощью тонкослойной хроматографии и жидкостной хроматографиимасс-спектрометрии экспериментально доказана продукция штаммом метаболитов липопептидной природы, относящихся к семейству итурина и сурфактина (итурин А, итурин А4, изомеры итурина А6-А7, сурфактин А, сурфактин С, изомеры сурфактина В).

Antimicrobial metabolites of bacterial strain B. amyloliquefaciens BIM B-1125, the basis of Bacto-health preparation, demonstrating antagonism against the representatives of the opportunistic microbiota of valuable fish species, were isolated and characterized. Extracellular localization of antimicrobial compounds was established; their stability is shown in the temperature range of 50–100 °С and in the pH range of 2–10. The lipopeptide nature of antimicrobial metabolites was shown using thin layer chromatography. The mass spectrometric analysis of the active fraction confirmed the production of lipopeptide metabolites belonging to the iturin and surfactin families (iturin A, iturin A4, iturin A6-A7 isomers, surfactin A, surfactin C, and surfactin B isomers).

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The authors declare that there are no conflicts of interest present.