danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2022-66-6-605-613dan-1100Research ArticleБИОЛОГИЯBIOLOGYСтимулирующее действие оксикоричных кислот и их конъюгатов с хитозаном на рост и биохимические показатели микроклонов картофеля in vitroEffect of the hydroxycinnamic acids and their chitosan-based conjugates on the growth and biochemical parameters of microclone potato plants in vitroЕловскаяН. А.YalouskayaN. A.

Еловская Нинель Анатольевна – аспирант.

ул. Академическая, 27, 220072, Минск

Ninel A. Yalouskaya – Postgraduate Student, V.F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus.

27, Akademicheskaya Str., 220072, Minsk

yalouskaya92@mail.ruКалацкаяЖ. Н.KalatskajaJ. N.

Калацкая Жанна Николаевна – кандидат биологических наук, доцент.

ул. Академическая, 27, 220072, Минск

Joanna N. Kalatskaja – Ph. D. (Biology), Associate Professor, V.F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus.

27, Akademicheskaya  Str.,  220072,  Minsk

kalatskayaj@mail.ruЛаманН. А.LamanN. A.

Ламан Николай Афанасьевич – академик, доктор биологических наук, профессор, заведующий лабораторией.

ул. Академическая, 27, 220072, Минск

Nikolai A. Laman – Academician, D. Sc. (Biology), Professor, Head of the Laboratory, V.F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus.

27, Akademicheskaya Str., 220072, Minsk

nikolai.laman@gmail.comГилевскаяК. С.HileuskayaK. S.

Гилевская Ксения Сергеевна – кандидат химических наук, доцент, старший научный сотрудник.

ул. Ф. Скорины, 36, 220141, Минск

Kseniya S. Hileuskaya – Ph. D. (Chemistry), Associate Professor, Senior Researcher, Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus.

36, F. Skorina Str., 220141, Minsk

k_hilevskay@mail.ruКуликовскаяВ. И.KulikouskayaV. I.

Куликовская Виктория Игоревна – кандидат химических наук, заведующий лабораторией.

ул. Ф. Скорины, 36, 220141, Минск

Viktoryia I. Kulikouskaya – Ph. D. (Chemistry), Associate Professor, Head of the Laboratory, Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus.

36, F. Skorina Str., 220141, Minsk

kulikouskaya@gmail.comНиколайчукВ. В.NikalaichukV. V.

Николайчук Виктория Викторовна  –  магистрант, младший научный сотрудник.

ул. Ф. Скорины, 36, 220141, Минск

Viktoryia V. Nikalaichuk – Master Student, Junior Researcher. Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus.

36, F. Skorina Str., 220141, Minsk

vica10bcn@gmail.comИнститут экспериментальной ботаники имени В.Ф. Купревича Национальной академии наук БеларусиV.F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of BelarusИнститут химии новых материалов Национальной академии наук БеларусиInstitute of Chemistry of New Materials of the National Academy of Sciences of Belarus202203012023666605613Copyright © Еловская Н.А., Калацкая Ж.Н., Ламан Н.А., Гилевская К.С., Куликовская В.И., Николайчук В.В., 20232023Еловская Н.А., Калацкая Ж.Н., Ламан Н.А., Гилевская К.С., Куликовская В.И., Николайчук В.В.Yalouskaya N.A., Kalatskaja J.N., Laman N.A., Hileuskaya K.S., Kulikouskaya V.I., Nikalaichuk V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1100

Рассматривается влияние кофейной и феруловой кислот и их конъюгатов на основе хитозана на ростовые и отдельные биохимические показатели микроклонов картофеля (Solanum tuberosum L.) сорта Бриз в культуре in vitro. Выявлен их положительный эффект на рост и развитие растений, при этом максимальное стимулирующее действие наблюдали у кофейной кислоты и ее конъюгата. Добавление хитозана в питательную среду культивирования вызвало развитие оксидативного стресса в клетках микроклональных растений. Оксикоричные кислоты проявили антиоксидантные свойства, снижая образование активных форм кислорода и стресс-индуцируемое накопление пролина. Конъюгат хитозана и кофейной кислоты действовал как умеренной силы стрессор, активизируя метаболизм и адаптационные процессы у формирующихся микроклональных растений.

This study determined the effect of exogenously applied hydroxycinnamic acids and their chitosan-based conjugates on growth and biochemical parameters of microclone potato plants (Solanum tuberosum L.) in vitro under optimal conditions. The findings of the present investigation confirm applying of chitosan-caffeic acid and chitosan-ferulic acid conjugates on microclone potato plants to improve growth and development. A significant growth-stimulating effect was found in caffeic acid and its chitosan-based conjugate. Chitosan behaved like a general elicitor inducing oxidative stress in plant cells. In turn, hydroxycinnamic acids acted as antioxidants to scavenge reactive oxygen species. The chitosan-caffeic acid conjugate can be considered as a low stress factor that activates metabolism and adaptation processes of microclonal potato plants.

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The authors declare that there are no conflicts of interest present.