danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2023-67-3-214-221dan-1131Research ArticleБИОЛОГИЯBIOLOGYKASP-генотипирование локусов, ассоциированных с признаком «масса 1000 зерен» мягкой пшеницы (Triticum aestivum L.)KASP genotyping of loci associated with the “1000-grain weight” trait in common wheat (Tríticum aestívum L.)ЛемешВ. А.LemeshV. A.

Лемеш Валентина Александровна – канд. биол. наук, доцент, заведующий лабораторией

ул. Академическая, 27, 220072, Минск, Республика Беларусь

Lemesh Valiantsina A. – Ph. D. (Biology), Assistant Professor, Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus

v.lemesh@igc.byГрибС. И.GrybS. I.

Гриб Станислав Иванович – академик, д-р с.-х. наук, профессор, гл. науч. сотрудник

ул. Тимирязева, 1, 222160, Минская обл., г. Жодино, Республика Беларусь

Stanislau Gryb – Academician, D. Sc. (Agrarian), Professor, Chief Researcher

1, Timiryazev Str., 222160, Zhodino, Minsk Region, Republic of Belarus

triticale@tut.byЛагуновскаяЕ. В.LagunovskayaA. V.

Лагуновская Елена Владимировна – ст. науч. сотрудник

ул. Академическая, 27, 220072, Минск, Республика Беларусь

Lahunovskaya Alena V. – Senior Researcher

27, Akademicheskaya Str., 220072, Minsk, Republicof Belarus

e.antonenko@igc.byКипеньВ. Н.KipenV. N.

Кипень Вячеслав Николаевич – канд. биол. наук, вед. науч. сотрудник

ул. Академическая, 27, 220072, Минск, Республика Беларусь

Kipen Viachaslau N. – Ph. D. (Biology), Leading Researcher

27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus

v.kipen@igc.byБулойчикА. А.BuloichikA. A.

Булойчик Андрей Александрович – канд. биол. наук, вед. науч. сотрудник

ул. Академическая, 27, 220072, Минск, Республика Беларусь

Buloichik Andrei A. – Ph. D. (Biology), Leading Researcher

27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus

a.buloichik@igc.byБуштевичВ. Н.BushtevichV. N.

Буштевич Виктор Николаевич – канд. с.-х. наук, доцент, заведующий лабораторией

ул. Тимирязева, 1, 222160, Минская обл., г. Жодино, Республика Беларусь

Bushtevich Victar N. – Ph. D. (Agrarian), Assistant Professor, Head of the Laboratory

1, Timiryazev Str., 222160, Zhodino, Minsk Region, Republic of Belarus

triticale@tut.byСаковичВ. И.SakovichV. I.

Сакович Валентина Ивановна – науч. сотрудник

ул. Академическая, 27, 220072, Минск, Республика Беларусь

Sakovich Valiantsina I. – Researcher

27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus

v.sakovich@igc.byИнститут генетики и цитологии Национальной академии наук БеларусиInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusНаучно-практический центр Национальной академии наук Беларуси по земледелиюResearch and Practical Center of the National Academy of Sciences of Belarus for Arable Farming202306072023673214221Copyright © Лемеш В.А., Гриб С.И., Лагуновская Е.В., Кипень В.Н., Булойчик А.А., Буштевич В.Н., Сакович В.И., 20232023Лемеш В.А., Гриб С.И., Лагуновская Е.В., Кипень В.Н., Булойчик А.А., Буштевич В.Н., Сакович В.И.Lemesh V.A., Gryb S.I., Lagunovskaya A.V., Kipen V.N., Buloichik A.A., Bushtevich V.N., Sakovich V.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1131

С использованием технологии KASP определен аллельный состав локусов TaTGW6-A1, TaGASR7-A1, TaCKX6-D1, TaGs3-D1, ассоциированных с признаком «масса 1000 зерен», у 25 сортов и сортообразцов мягкой яровой пшеницы белорусской и зарубежной селекции. Подобраны аннотированные ДНК последовательности для моделирования и синтеза праймеров KASP. Показано, что исследованные генотипы несут как благоприятные аллели, ассоциированные с увеличением массы 1000 зерен, так и аллели, оказывающие негативное влияние на исследуемый признак. Выделено 6 образцов пшеницы белорусской селекции, несущих комплекс аллелей, положительно коррелирующих со значением массы 1000 зерен: сорт Весточка-17, сортообразцы Э-2318, Э-2263, Э-2298, Э-1569, Э-2695.

Using the KASP technology, the allelic composition of TaTGW6-A1, TaGASR7-A1, TaCKX6-D1, and TaGs3-D1 loci associated with the 1000-grain weight trait was identified in 25 varieties and variety accessions of the common wheat of Belarusian and foreign breeding. Annotated DNA sequences were selected for modeling and synthesis of KASP primers. It was demonstrated that the examined genotypes had carried both favorable alleles associated with 1000-grain weight, and the alleles producing a negative effect on the trait under study. Six wheat accessions of Belarusian breeding were identified. They carried a complex of alleles positively correlating with the 1000-grain weight trait: variety Vestochka-17, accessions E-2318, E-2263, E-2298, E-1569, and E-2695.

мягкая яровая пшеницамасса 1000 зеренмолекулярные маркерыгенлокусаллельKASPгенотипированиеcommon wheat1000-grain weightmolecular markersa genea locusalleleKASP genotypingReferencesAbiotic stress signaling in wheat – an inclusive overview of hormonal interactions during abiotic stress responses in wheat / K. Abhinandan [et al.] // Front. Plant Sci. – 2018. – Vol. 9. – Art. 734. https://doi.org/10.3389/fpls.2018.00734Abhinandan K., Skori L., Stanic M., Hickerson N. M. N., Jamshed M., Samuel M. A. Abiotic stress signaling in wheat – an inclusive overview of hormonal interactions during abiotic stress responses in wheat. Frontiers in Plant Science, 2018, vol. 9, art. 734. https://doi.org/10.3389/fpls.2018.00734SNP Markers and Their Impact on Plant Breeding / J. Mammadov [et al.] // Int. J. Plant Genomics. – 2012. – Vol. 2012. – Art. 728398. https://doi.org/10.1155/2012/728398Mammadov J., Aggarwal R., Buyyarapu R., Kumpatla S. SNP Markers and Their Impact on Plant Breeding. International Journal of Plant Genomics, 2012, vol. 212, art. 728398. https://doi.org/10.1155/2012/728398Morgil, H. Single Nucleotide Polymorphisms (SNPs) in Plant Genetics and Breeding / H. Morgil, Y. Gercek, I. Tulum // The Recent Topics in Genetic Polymorphisms. – 2020. https://doi.org/10.5772/intechopen.91886Morgil H., Gercek Y., Tulum I. Single Nucleotide Polymorphisms (SNPs) in Plant Genetics and Breedinng. The Recent Topics in Genetic Polymorphisms, 2020. https://doi.org/10.5772/intechopen.91886Utilization of KASP technology for wheat improvement / B. Kaur [et al.] // Cereal Res. Commun. – 2020. – Vol. 48, N 4. – P. 409–421. https://doi.org/10.1007/s42976-020-00057-6Kaur B., Mavi G. S., Gill M. S., Saini D. K. Utilization of KASP technology for wheat improvement. Cereal Research Communications, 2020, vol. 48, no. 4, pp. 409–421. https://doi.org/10.1007/s42976-020-00057-6Global status of 47 major wheat loci controlling yield, quality, adaptation and stress resistance selected over the last century / J. Zhao [et al.] // BMC Plant Biol. – 2019. – Vol. 19, N 1. – Art. 5. https://doi.org/10.1186/s12870-018-1612-yZhao J., Wang Z., Liu H., Zhao J., Li T., Hou J., Zhang X., Hao C. Global status of 47 major wheat loci controlling yield, quality, adaptation and stress resistance selected over the last century. BMC Plant Biology, 2019, vol. 19, no. 1, art. 5. https://doi.org/10.1186/s12870-018-1612-yMolecular Characterization of 87 Functional Genes in Wheat Diversity Panel and Their Association with Phenotypes under Well-Watered and Water-Limited Conditions / M. Khalid [et al.] // Front. Plant Sci. – 2019. – Vol. 10. – Art. 717. https://doi.org/10.3389/fpls.2019.00717Khalid M., Afzal F., Gul A., Amir R., Subhani A., Ahmed Z., Mahmood Z., Xia X., Rasheed A., He Z. Molecular Characterization of 87 Functional Genes in Wheat Diversity Panel and Their Association with Phenotypes under Well-Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL / J. Wei [et al.] // Theor. Appl. Genet. – 2015. – Vol. 128, N 10. – P. 2067–2076. https://doi.org/10.1007/s00122-015-2567-0Watered and Water-Limited Conditions. Frontiers in Plant Science, 2019, vol. 10, art. 717. https://doi.org/10.3389/fpls.2019.00717MASWheat [Electronic resource]. – Mode of access: https:// https://maswheat.ucdavis.edu/. – Date of access: 06.09.2022.Wei J., Geng H., Zhang Y., Liu J., Wen W., Zhang Y., Xia X., Chen X., He Z. Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL. Theoretical and Applied Genetics, 2015, vol. 128, no. 10, pp. 2067–2076. https://doi.org/10.1007/s00122-015-2567-0Variation in allelic frequencies at loci associated with kernel weight and their effects on kernel weight-related traits in winter wheat / T. Li [et al.] // Crop. J. – 2019. – Vol. 7, N 1. – P. 30–37. https://doi.org/10.1016/j.cj.2018.08.002MASWheat (2022) Available at: https://maswheat.ucdavis.edu (accessed 6 September 2022).Genome-Wide Association Study of Kernel Traits Using a 35K SNP Array in Bread Wheat (Triticum aestivum L.) / P. Wang [et al.] // Front. Plant Sci. – 2022. – Vol. 13. – Art. 905660. https://doi.org/10.3389/fpls.2022.905660Li T., Liu H., Mai C., Yu G., Li H., Meng L., Jian D., Yang L., Zhou Y., Zhang H.,, Li H. Variation in allelic frequencies at loci associated with kernel weight and their effects on kernel weight-related traits in winter wheat. The Crop Journal, 2019, vol. 7, no. 1, pp. 30–37. https://doi.org/10.1016/j.cj.2018.08.002Дорохов, Д. Б. Быстрая и экономичная технология RAPD анализа растительных геномов / Д. Б. Дорохов, Э. Клоке // Генетика. – 1997. – Т. 33, № 4. – С. 443–450.Wang P., Tian T., Ma J., Liu Y., Zhang P., Chen T., Shahinnia F., Yang D. Genome-Wide Association Study of Kernel Traits Using a 35K SNP Array in Bread Wheat (Triticum aestivum L.). Frontiers in Plant Science, 2022, vol. 13, art. 905660. https://doi.org/10.3389/fpls.2022.905660TaTGW6-A1, an ortholog of rice TGW6, is associated with grain weight and yield in bread wheat / M. Hanif [et al.] // Mol. Breed. – 2016. – Vol. 36, N. 1. https://doi.org/10.1007/s11032-015-0425-zDorokhov D. B., Klocke E. A rapid and economic technique for rapd analysis of plant genomes. Russian Journal of Genetics, 1997, vol. 33, no. 4, pp. 358–365.Natural variation of TaGASR7-A1 affects grain length in common wheat under multiple cultivation conditions / L. Dong [et al.] // Mol. Breed. – 2014. – Vol. 34, N 3. – P. 937–947. https://doi.org/10.1007/s11032-014-0087-2Hanif M., Gao F., Liu J., Wen W., Zhang Y., Rasheed A., Xia X., He Z., Cao S. TaTGW6-A1, an ortholog of rice TGW6, is associated with grain weight and yield in bread wheat. Molecular Breeding, 2016, vol. 36, art. 1. https://doi.org/10.1007/s11032-015-0425-zSpecificity of expression of TaCKX family genes in developing plants of wheat and their co-operation within and among organs / H. Ogonowska [et al.] // PLoS One. – 2019. – Vol. 14, N 4. – Art. e0214239. https://doi.org/10.1371/journal.pone.0214239Dong L., Wang F., Liu T., Dong Z., Li A., Jing R., Mao L., Li Y., Liu X., Zhang K., Wang D. Natural variation of TaGASR7-A1 affects grain length in common wheat under multiple cultivation conditions. Molecular Breeding, 2014, vol. 34, no. 3, pp. 937–947. https://doi.org/10.1007/s11032-014-0087-2Cloning, characterization of TaGS3 and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.) / J. Yang [et al.] // BMC Genet. – 2019. – Vol. 20, N 1. – Art. 98. https://doi.org/10.1186/s12863-019-0800-6Ogonowska H., Barchacka K., Gasparis S., Jablonski B., Orczyk W., Dmochowska-Boguta M., Nadolska-Orczyk A. Specificity of expression of TaCKX family genes in developing plants of wheat and their co-operation within and among organs. PLoS One, 2019, vol. 14, no. 4, art. e0214239. https://doi.org/10.1371/journal.pone.0214239Yang J., Zhou Y., Zhang Y., Hu W., Wu Q., Chen Y., Wang X., Guo G., Liu Z., Cao T., Zhao H. Cloning, characterization of TaGS3 and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.). BMC Genetics, 2019, vol. 20, no. 1, art. 98. https://doi.org/10.1186/s12863-019-0800-6Yang J., Zhou Y., Zhang Y., Hu W., Wu Q., Chen Y., Wang X., Guo G., Liu Z., Cao T., Zhao H. Cloning, characterization of TaGS3 and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.). BMC Genetics, 2019, vol. 20, no. 1, art. 98. https://doi.org/10.1186/s12863-019-0800-6

The authors declare that there are no conflicts of interest present.