danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2023-67-4-307-314dan-1142Research ArticleМЕДИЦИНАMEDICINEДвунитевые разрывы ДНК спермиев у пациентов с нормозооспермией и патозооспермиейSperm double-strand DNA breaks in patients with normozoospermia and pathozoospermia.КужельИ. Д.KuzhalI. D.

Кужель Ирена Дмитриевна – младший научный сотрудник

ул. Академическая, 27, 220072, Минск

Kuzhal Irena D. – Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

i.hileuskaya@igc.byПрибушеняО. В.PribushenyaO. V.

Прибушеня Оксана Владимировна – доктор медицинских наук, доцент, заведующий лабораторией

ул. Орловская, 66, 220053, Минск

Pribushenya Oksana V. – D. Sc. (Medicine), Associate Professor, Head of the Laboratory

66, Orlovskaya Str., 220053, Minsk

pribushenya@yandex.ruНаумчикИ. В.NaumchikI. V.

Наумчик Ирина Всеволодовна – кандидат медицинских наук, заместитель директора

ул. Орловская, 66, 220053, Минск

Naumchik Irina V. – Ph. D. (Medicine), Deputy Director

66, Orlovskaya Str., 220053, Minsk

belgenetics@yahoo.comКурловичИ. В.KurlovichI. V.

Курлович Иван Васильевич – кандидат медицинских наук, заместитель директора

ул. Орловская, 66, 220053, Минск

Kurlovich Ivan V. – Ph. D. (Medicine), Deputy Director

66, Orlovskaya Str., 220053, Minsk

info@medcenter.byРябоконьН. И.RyabokonN. I.

Рябоконь Надежда Ивановна – кандидат биологических наук, заведующий лабораторией

ул. Академическая, 27, 220072, Минск

Ryabokon Nadezhda I. – Ph. D. (Biology), Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk

n.ryabokon@igc.byИнститут генетики и цитологии Национальной академии наук БеларусиInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusРеспубликанский научно-практический центр «Мать и дитя»Scientific and Practical Center “Mother and Child”202301092023674307314Copyright © Кужель И.Д., Прибушеня О.В., Наумчик И.В., Курлович И.В., Рябоконь Н.И., 20232023Кужель И.Д., Прибушеня О.В., Наумчик И.В., Курлович И.В., Рябоконь Н.И.Kuzhal I.D., Pribushenya O.V., Naumchik I.V., Kurlovich I.V., Ryabokon N.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1142

С использованием нейтральной версии метода ДНК-комет изучены уровни двунитевых разрывов ДНК − грубых нарушений целостности генома – в образцах спермы мужчин из белорусской популяции при нормозооспермии и патозооспермии, включая астенозооспермию, олигозооспермию, олигоастенозооспермию и другие смешанные формы патозооспермии. Показано, что двунитевые разрывы ДНК составляют большую долю (в среднем около 44–50 %) от общего количества повреждений ДНК (однои двунитевых разрывов, щелочно-лабильных сайтов и др.), регистрируемых с использованием щелочной версии метода ДНК-комет, рекомендованной Всемирной организацией здравоохранения. Установлены более высокие уровни двунитевых разрывов ДНК спермиев в группе с патозооспермией и подгруппе с астенозооспермией, чем при нормозооспермии. Показано также, что зарегистрированные при патозооспермии уровни двунитевых разрывов соответствуют мутагенному эффекту in vitro высоких концентраций (10–30 мкг/мл) мощного радиомиметика сульфата блеомицина, что свидетельствует о существенном нарушении целостности ДНК при патозооспермии. В целом полученные данные говорят о возможности использования анализа двунитевых разрывов ДНК спермиев в диагностике мужского бесплодия.

The levels of double-strand DNA breaks as a severe disruption of genome integrity were studied using the neutral version of the comet assay in the sperm samples of the men of the Belarusian population with normozoospermia and pathozoospermia, including asthenozoospermia, oligozoospermia, oligoasthenozoospermia, and other combined forms of pathozoospermia. It was demonstrated that double-strand DNA breaks have a large proportion (about 44–50 % on average) of the total number of DNA damage (singleand double-strand breaks, alkaline-labile sites, etc.) analyzed with the alkaline version of the comet assay recommended by the World Health Organization. Higher levels of sperm double-strand DNA breaks were established in the pathozoospermia group and in asthenozoospermia subgroup compared to the normozoospermia group. It was also shown that the levels of double-strand breaks observed at pathozoospermia correspond to the mutagenic effect in vitro of high concentrations (10–30 μg/ml) of bleomycin sulfate that acts as a strong radiomimetic, which points to a significant disruption of the DNA integrity at pathozoospermia. In general, the data obtained demonstrate the usefulness of the sperm double-strand DNA break analysis for male infertility diagnostics.

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The authors declare that there are no conflicts of interest present.