danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2024-68-2-148-155dan-1185Research ArticleМЕДИЦИНАMEDICINEРаспространенность генов сериновых карбапенемаз среди множественно-резистентных штаммов Klebsiella pneumoniae, выделенных у пациентов отделений хирургии и реанимацииPrevalence of serine carbapenemase genes among multi-resistant strains of Klebsiella pneumoniae isolated from patients in the departments of surgery and intensive care unitshttps://orcid.org/0000-0003-1726-1031ЯновичО. О.YanovichO. O.

Янович Ольга Олеговна – канд. биол. наук, вед. науч. сотрудник

ул. Филимонова, 23, 220114, Минск

Olga O. Yanovich – Ph. D. (Biology), Leading Researcher

23, Filimonov Str., 220114, Minsk

oyanov74@mail.ruhttps://orcid.org/0000-0002-4524-3730ТитовЛ. П.TitovL. P.

Титов Леонид Петрович – академик, д-р мед. наук, профессор, заведующий лабораторией

ул. Филимонова, 23, 220114, Минск

Leonid P. Titov – Academician, D. Sc. (Medicine), Professor, Head of the Laboratory

23, Filimonov Str., 220114, Minsk

leotit310@gmail.comРеспубликанский научно-практический центр эпидемиологии и микробиологииRepublican Research and Practical Center for Epidemiology and Microbiology202429042024682148155Copyright © Янович О.О., Титов Л.П., 20242024Янович О.О., Титов Л.П.Yanovich O.O., Titov L.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1185

Оценена распространенность и сочетание генов сериновых карбапенемаз среди фенотипически множественно-резистентных штаммов K. pneumoniae, выделенных у пациентов отделений хирургии и реанимации различных регионов Республики Беларусь. Методом ПЦР было проведено определение наличия генов сериновых карбапенемаз (KPC, OXA-48, OXA-23, OXA-24) у 115 штаммов фенотипически множественно-резистентных К. pneumoniae. Среди изолятов K. pneumoniae чаще всего выявлялся ген blaОХА-48 – 70 %, гены карбапенемаз blaKРС и blaOXA-24 были детектированы с практически равной частотой 43,6 и 39,1 %. Показано, что у 23,5 % штаммов К. pneumoniae выявлены гены сериновых бета-лактамаз в качестве единственной детерминанты резистентности, а в остальных случаях клебсиеллы являлись носителями генов нескольких типов бета-лактамаз.

The frequency of serine carbapenemases in phenotypically multi-drug resistant K. pneumoniae obtained from patients of surgery and intensive care units in Belarus was assessed. Beta-lactamase genes KPC, OXA-48, OXA-23, and OXA-24 were detected by PCR in 115 phenotypically multi-drug resistant K. pneumoniae. The most common carbapenemase gene was blaOXA-48 (70 %). The genes KPC and blaOXA-24 were identified in 43.6 and 39.1 % isolates, respectively. Single serine carbapenemase gene was detected in 23.5 % strains. On other strains, the molecular analysis determined double or triple carbapenemase gene combinations.

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The authors declare that there are no conflicts of interest present.