danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2024-68-6-465-473dan-1223Research ArticleБИОЛОГИЯBIOLOGYРазработка лентивирусной векторной системы для эктопической экспрессии и презентации меланомного антигена MLANA в клетках человекаDevelopment of the lentivirusbased vector system for ectopic expression and presentation of the melanoma antigen MLANA in human cellshttps://orcid.org/0000-0001-9535-7157АнтоневичН. Г.AntonevichN. G.

Антоневич Наталья Георгиевна – канд. биол. наук, доцент, вед. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Antonevich Natalia G. – Ph. D. (Biology), Associate Professor, Leading Researche

27, Akademicheskaya Str., 220072, Minsk

antonevich.n@gmail.comhttps://orcid.org/0009-0002-8909-2767БадалянИ. А.BadalyanI. A.

Бадалян Ирина Артуровна – мл. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Badalyan Iryna A. – Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

irina1512.37533@gmail.comhttps://orcid.org/0000-0002-4869-9864ГончаровА. Е.HancharouA. Y.

Гончаров Андрей Евгеньевич – канд. мед. наук, доцент, директор

ул. Академическая, 27, 220072, Минск

Hancharou Andrei Y. – Ph. D. (Medicine), Associate Professor, Director

27, Akademicheskaya Str., 220072, Minsk

andrei.hancharou@gmail.comhttps://orcid.org/0000-0002-4524-3730ТитовЛ. П.TitovL. P.

Титов Леонид Петрович – академик, д-р мед. наук, профессор, гл. науч. сотрудник

ул. Казинца, 50, 220099, Минск

Titov Leonid P. – Academician, D. Sc. (Medicine), Professor, Chief Researcher

50, Kazinets Str., 220099, Minsk

leotit310@gmail.comhttps://orcid.org/0000-0001-9981-7333ГриневВ. В.GrinevV. V.

Гринев Василий Викторович – канд. биол. наук, доцент

ул. Курчатова, 10, 220045, Минск

Grinev Vasily V. – Ph. D. (Biology), Associate Professor

10, Kurchatov Str., 220045, Minsk

grinev_vv@bsu.byИнститут биофизики и клеточной инженерии Национальной академии наук БеларусиInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of BelarusРеспубликанский центр гигиены, эпидемиологии и общественного здоровьяRepublican Center for Hygiene, Epidemiology and Public HealthБелорусский государственный университетBelarusian State University202408012025686465473Copyright © Антоневич Н.Г., Бадалян И.А., Гончаров А.Е., Титов Л.П., Гринев В.В., 20252025Антоневич Н.Г., Бадалян И.А., Гончаров А.Е., Титов Л.П., Гринев В.В.Antonevich N.G., Badalyan I.A., Hancharou A.Y., Titov L.P., Grinev V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1223

Освоены и испытаны лентивирусные векторы экспрессии, кодирующие два генно-инженерных варианта белка MLANA, названных MLANA-FLAG® и MLANA-FLAG®-LAMP1. Транскрипционная/трансляционная активность сконструированных последовательностей в клетках линии HEK 293T и дендритных клетках подтверждена методами флуоресцентной конфокальной микроскопии и проточной цитометрии. Созданные генетические конструкции могут быть использованы для генетической модификации дендритных клеток и разработки новых методов клеточной терапии меланомы.

Lentivirus-based vectors encoding two engineered melanoma associated MLANA proteins, named MLANAFLAG® and MLANA-FLAG®-LAMP1, were developed and tested. Transcriptional and translational activity of engineered proteins was confirmed in HEK 293T cells and dendritic cells by fluorescence confocal microscopy and flow cytometry. These new vectors can be used for lentiviral transduction of dendritic cells and the creation of new cell therapies for human melanoma.

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The authors declare that there are no conflicts of interest present.