danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2025-69-2-129-136dan-1244Research ArticleБИОЛОГИЯBIOLOGYАнализ однонуклеотидного полиморфизма c применением технологии KASP для идентификации пород домашних свинейAnalysis of single nucleotide polymorphism using KASP technology for identification of domestic pigs breedshttps://orcid.org/0000-0002-7822-0746КипеньВ. Н.KipenV. N.

Кипень Вячеслав Николаевич – канд. биол. наук, доцент, вед. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Kipen Viachaslau N. – Ph. D. (Biology), Associate Professor, Leading Researcher

27, Akademicheskaya Str., 220072, Minsk

v.kipen@igc.byСнытковЕ. В.SnytkovE. V.

Снытков Евгений Владимирович – науч. сотрудник

ул. Академическая, 27, 220072, Минск

Snytkov Evgenij V. – Researcher

27, Akademicheskaya Str., 220072, Minsk

https://orcid.org/0000-0001-6087-5069МихайловаМ. Е.MikhailovaM. E.

Михайлова Мария Егоровна – канд. биол. наук, доцент, заведующий лабораторией

ул. Академическая, 27, 220072, Минск

Mikhailova Mariya E. – Ph. D. (Biology), Associate Professor, Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk

M.Mikhailova@igc.byhttps://orcid.org/0000-0001-5442-566XШейкоР. И.SheykoR. I.

Шейко Руслан Иванович – член-корреспондент, д-р с.-х. наук, профессор, гл. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Sheyko Ruslan I. – Corresponding Member, D. Sc. (Agrarian), Professor, Chief Researcher

27, Akademicheskaya Str., 220072, Minsk

R.I.Sheyko@igc.byИнститут генетики и цитологии Национальной академии наук БеларусиInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus202506052025692129136Copyright © Кипень В.Н., Снытков Е.В., Михайлова М.Е., Шейко Р.И., 20252025Кипень В.Н., Снытков Е.В., Михайлова М.Е., Шейко Р.И.Kipen V.N., Snytkov E.V., Mikhailova M.E., Sheyko R.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1244

Впервые в Республике Беларусь с целью дифференциации пород свиней дюрок, ландрас, йоркшир, белорусская крупная белая и белорусская мясная проведен широкомасштабный биоинформатический анализ геномов вида Sus scrofa domesticus, а также собственные молекулярно-генетические исследования, по результатам которых сформирован перечень полиморфных вариантов с высоким дифференцирующим потенциалом. Наиболее информативные SNP для дифференциации пород свиней – rs332196135, rs81322965, rs322056535, rs80967182, rs81333725, rs80789418, rs319844693, rs80859281, rs80855833, вошли в тест-системы. На основании статистического анализа генотипов, полученных in silico и с помощью технологии конкурентной аллель-специфической ПЦР, определены высокие значения точности и специфичности предложенных моделей. Разработаны методические рекомендации для быстрой и точной дифференциации пород свиней.

For the first time in the Republic of Belarus, a large-scale bioinformatic analysis of the genomes of Sus scrofa domesticus species, as well as our own molecular genetic studies, was conducted in order to differentiate Duroc, Landrace, Yorkshire, Belarusian Large White, and Belarusian Meat pig breeds. As a result, a list of polymorphic variants with high differentiating potential was formed. The most informative SNPs for differentiating pig breeds are rs332196135, rs81322965, rs322056535, rs80967182, rs81333725, rs80789418, rs319844693, rs80859281, rs80855833. Based on the statistical analysis of genotypes obtained in silico and using competitive allele-specific PCR technology, high values of accuracy and specificity of the proposed models were determined. Methodological recommendations have been developed for the rapid and accurate differentiation of pig breeds.

Sus scrofa domesticusдюрокландрасйоркширбелорусская крупная белаябелорусская мяснаяоднонуклеотидный полиморфизмдифференциацияконкурентная аллель-специфическая ПЦРгенотипирование in silicoSus scrofa domesticusDurocLandraceYorkshireBelarusian Large WhiteBelarusian Meatsingle nucleotide polymorphismdifferentiationcompetitive allele-specific PCRin silico genotypingМолекулярно-генетические исследования выполнены в рамках ГПНИ «Биотехнологии-2» (2021–2025 гг.), подпрограмма «Геномика, эпигеномика, биоинформатика», НИР «Разработка системы генетического анализа для определения чистопородности свиней на основе изучения SNP-локусов» (№ 20210339). Программное обеспечение GENIS разработано в рамках НИР «Биоинформатический подход к анализу данных полногеномного секвенирования для поиска однонуклеотидных замен, способных дифференцировать близкородственные биологические виды» (БРФФИ, 2023–2025 гг., Б23-060).The study was carried out within the framework of the State Scientific Research Program “Biotechnology-2” (2021–2025), subprogram “Genomics, epigenomics, bioinformatics”, research work “Development of a genetic analysis system for determining the purity of pigs based on the study of SNP loci” (No. 20210339). 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The authors declare that there are no conflicts of interest present.