danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2025-69-4-314-321dan-1265Research ArticleМЕДИЦИНАMEDICINEСравнительный анализ профиля экспрессии молекулярных маркеров мезенхимальных стволовых клеток, полученных из тканей плацентарно-пуповинного комплекса, жировой ткани и костного мозгаComparative analysis of the profile of expression of molecular markers of mesenchymal stem cells obtained from placental-umbilical cord complex tissues, adipose tissue and bone marrowhttps://orcid.org/0000-0002-0966-7456ЮркинаЕ. Г.YurkinaE. G.

Юркина Екатерина Геннадьевна – ст. науч. cотрудник

ул. Семашко, 8, 220087, Минск

Yurkina Ekaterina G. – Senior Researcher

8, Semashko Str., 220087, Minsk

https://orcid.org/0000-0002-6813-4465КривенкоС. И.KrivenkoS. I.

Кривенко Светлана Ивановна – д-р мед. наук, профессор, заместитель директора

ул. Семашко, 8, 220087, Минск

 

Krivenko Svetlana I. – D. Sc. (Medicine), Professor, Deputy Director

8, Semashko Str., 220087, Minsk

https://orcid.org/0000-0001-5947-8285СмольниковаВ. В.SmolnikovaV. V.

Смольникова Виктория Владимировна – канд. биол. наук, доцент, вед. науч. сотрудник

ул. Семашко, 8, 220087, Минск

Smolnikova Victoria V. – Ph. D. (Biology), Associate Professor, Leading Researcher

8, Semashko Str., 220087, Minsk

https://orcid.org/0000-0002-3011-2287ПримаковаЕ. А.PrimakovaE. A.

Примакова Евгения Алексеевна – врач

ул. Семашко, 8, 220087, Минск

Primakova Evgeniya A. – Doctor

8, Semashko Str., 220087, Minsk

https://orcid.org/0000-0001-7147-4834НазароваЕ. А.NazarovaE. A.

Назарова Екатерина Александровна – канд. биол. наук, врач

ул. Семашко, 8, 220087, Минск

Nazarova Ekaterina A. – Ph. D. (Biology), Doctor

8, Semashko Str., 220087, Minsk

https://orcid.org/0000-0003-1521-1620СымановичА. А.SymanovichA. A.

Сыманович Алла Александровна – канд. биол. наук, доцент, врач

ул. Семашко, 8, 220087, Минск

Symanovich Alla A. – Ph. D. (Biology), Associate Professor, Doctor

8, Semashko Str., 220087, Minsk

https://orcid.org/0000-0001-9807-2093ДедюляН. И.DedyulyaN. I.

Дедюля Наталья Ивановна – канд. биол. наук, доцент, заведующий лабораторией

ул. Семашко, 8, 220087, Минск

Dedyulya Natalia I. – Ph. D. (Biology), Associate Professor, Head of the Laboratory

8, Semashko Str., 220087, Minsk

https://orcid.org/0000-0001-6577-9738РомановаИ. А.RomanovaI. A.

Романова Ирина Александровна – науч. сотрудник

ул. Семашко, 8, 220087, Минск

Romanova Irina A. – Researcher

8, Semashko Str., 220087, Minsk

https://orcid.org/0000-0001-6834-8538ЕфимовД. Ю.EfimovD. Yu.

Ефимов Денис Юрьевич – канд. мед. наук, доцент, врач-хирург

ул. Семашко, 8, 220087, Минск

Efimov Denis Yu. – Ph. D. (Medicine), Associate Professor, Surgeon

8, Semashko Str., 220087, Minsk

https://orcid.org/0000-0003-0569-6150ЩербаА. Е.ShcherbaA. E.

Щерба Алексей Евгеньевич – д-р мед. наук, профессор, заместитель директора

ул. Семашко, 8, 220087, Минск

Shcherba Aliaksey E. – D. Sc. (Medicine), Professor, Deputy Director

8, Semashko Str., 220087, Minsk

https://orcid.org/0000-0001-7023-4767РуммоО. О.RummoO. O.

Руммо Олег Олегович – академик, д-р мед. наук, профессор, директор

ул. Семашко, 8, 220087, Минск

Rummo Oleg O. – Academician, D. Sc. (Medicine), Professor, Director

8, Semashko Str., 220087, Minsk

Минский научно-практический центр хирургии, трансплантологии и гематологииMinsk Scientific and Practical Center for Surgery, Transplantology and Hematology202528082025694314–321Copyright © Юркина Е.Г., Кривенко С.И., Смольникова В.В., Примакова Е.А., Назарова Е.А., Сыманович А.А., Дедюля Н.И., Романова И.А., Ефимов Д.Ю., Щерба А.Е., Руммо О.О., 20252025Юркина Е.Г., Кривенко С.И., Смольникова В.В., Примакова Е.А., Назарова Е.А., Сыманович А.А., Дедюля Н.И., Романова И.А., Ефимов Д.Ю., Щерба А.Е., Руммо О.О.Yurkina E.G., Krivenko S.I., Smolnikova V.V., Primakova E.A., Nazarova E.A., Symanovich A.A., Dedyulya N.I., Romanova I.A., Efimov D.Y., Shcherba A.E., Rummo O.O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1265

Мезенхимальные стволовые клетки (МСК) представляют собой популяцию мультипотентных клеток, которые обладают иммуномодулирующими, противовоспалительными и антифибротическими свойствами, а также способностью к секреции трофических факторов, которые способствуют регенерации поврежденных тканей, что обосновывает их широкое клиническое применение. Существует необходимость изучения молекулярного профиля гетерогенности МСК для их более эффективного применения в клеточной терапии различных патологий. Изучен молекулярный профиль МСК из плацентарно-пуповинного комплекса (децидуальная ткань, хориальная пластинка, ворсинки хориона, амниотическая мембрана и пупочный канатик), жировой ткани и костного мозга. Дана оценка уровня экспрессии маркеров клеточной поверхности, которые характеризуют гетерогенность МСК, полученных из различных источников. МСК из всех тканей экспрессировали на своей поверхности типичные маркеры МСК, включая CD13, CD73, CD105 и CD90, при отсутствии экспрессии гемопоэтических маркеров (CD14, CD34 и CD45), костимулирующей молекулы CD86, мембранного белка CD154, молекулы адгезии CD15, маркеры эмбриональных стволовых клеток (TRA-1-81 и TRA-1-60R). Однако они отличались экспрессией маркеров стволовости (Oct-4, Nanog), коингибиторных молекул (CD273, CD274 и CD200), молекулы клеточной адгезии CD146, поверхностного антигена SSEA-4, а также фактора транскрипции GATA4.

Mesenchymal stem cells (MSCs) are multifunctional adult stem cells that can be derived from various tissues, such as adult adipose tissue, peripheral blood, bone marrow, neonatal placenta, and umbilical cord tissue. MSCs have the capacity for self-renewal and multidirectional differentiation. Despite the absence of differences in the expression of the main surface markers, MSCs obtained from tissues of the placenta-umbilical cord complex, adipose tissue and bone marrow differ significantly in their molecular profile. Cellular heterogeneity is ubiquitous across MSC cultures from different species and tissues. During the course of the study, the expression level of cell surface markers that characterize the heterogeneity of MSCs obtained from various sources was assessed. MSCs from all tissues express typical MSC markers on their surface, including CD13, CD73, CD105 and CD90, in the absence of expression of hematopoietic markers (CD14, CD34 and CD45), costimulatory molecules CD86, membrane protein CD154, adhesion molecules CD15, and markers of embryonic stem cells (TRA-1-81 and TRA-1-60R). However, they differed in the expression of stemness markers (October 4, Nanog), coinhibitory molecules (CD273, CD274, and CD200), cell adhesion molecules CD146, surface antigen SSEA-4, and transcription factor GATA4. The results on the molecular profile of MSCs as a heterogeneous cell population allow for the scientific substantiation of the choice of tissue source for the creation of biomedical cell products with specified therapeutic properties.

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The authors declare that there are no conflicts of interest present.