danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2026-70-1-45-53dan-1293Research ArticleБИОЛОГИЯBIOLOGYЗащитное действие кверцетина, инкапсулированного в желатиновые наночастицы, при инициировании клеточного окислительного стрессаProtective effect of quercetin encapsulated in gelatin nanoparticles against induced cellular oxidative stressПотаповичА. И.PotapovichA. I.

Потапович Алла Ивановна – канд. биол. наук, доцент, вед. науч. сотрудник.

Пр. Независимости, 4, 220030, Минск

Potapovich Alla I. – Ph. D. (Biology), Associate Professor, Leading Researcher.

4, Nezavisimosti Ave., 220030, Minsk

pot-alla@rambler.ruКостюкТ. В.KostyukT. V.

Костюк Татьяна Владимировна – науч. сотрудник.

Пр. Независимости, 4, 220030, Минск

Kostyuk Tatyana V. – Researcher.

4, Nezavisimosti Ave., 220030, Minsk

tanyasuhan@mail.ruШутоваТ. Г.ShutovaT. G.

Шутова Татьяна Геннадьевна – канд. хим. наук, вед. науч. сотрудник.

Ул. Ф. Скорины, 36, 220084, Минск

Shutava Tatsiana G. – Ph. D. (Chemistry), Leading Researcher.

36, F. Skorinа Str., 220084, Minsk

shutova@ichnm.basnet.byКостюкВ. А.KostyukV. A.

Костюк Владимир Андреевич – д-р хим. наук, профессор, заведующий лабораторией.

Пр. Независимости, 4, 220030, Минск

Kostyuk Vladimir A. – D. Sc. (Chemistry), Professor, Head of the Laboratory.

4, Nezavisimosti Ave., 220030, Minsk

kostyuk@bsu.byБелорусский государственный университетBelarusian State UniversityИнститут химии новых материалов Национальной академии наук БеларусиInstitute of Chemistry of New Materials of the National Academy of Sciences of Belarus2026020320267014553Copyright © Потапович А.И., Костюк Т.В., Шутова Т.Г., Костюк В.А., 20262026Потапович А.И., Костюк Т.В., Шутова Т.Г., Костюк В.А.Potapovich A.I., Kostyuk T.V., Shutova T.G., Kostyuk V.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1293

Исследование проводилось с целью оценки перспективности применения желатиновых наночастиц в качестве средства повышения эффективности и биодоступности фитосоединений, в частности кверцетина. Установлено, что инкапсуляция кверцетина в желатиновые наночастицы позволяет использовать его в виде водных суспензий, не снижая антиоксидантный потенциал, генои цитопротекторное действие при моделировании клеточного окислительного стресса, что свидетельствует о высокой эффективности высвобождения кверцетина из данного наноносителя. Продемонстрировано, что использование желатиновых наночастиц представляет собой перспективный подход для повышения биодоступности и терапевтической эффективности фитосоединений.

The aim of this study was to evaluate the potential of gelatin nanoparticles as a means of increasing the efficacy and bioavailability of phytocompounds, in particular quercetin. The protective effect of native quercetin and quercetin incorporated into gelatin nanoparticles was studied by initiating oxidative stress in human keratinocytes with tert-butyl hydroperoxide. Cell viability was assessed using the PrestoBlueTM reagent, apoptotic and necrotic cells were detected by double intravital staining using an assay kit including annexin V-FITC. DNA damage was analyzed using the comet assay. The results demonstrated that encapsulation of quercetin into gelatin nanoparticles enables its application in aqueous suspensions without compromising its antioxidant capacity, geneand cytoprotective effects under cellular oxidative stress conditions, indicating a high efficiency of quercetin release from this nanocarrier. Consequently, the utilization of gelatin nanoparticles represents a promising approach for improving the bioavailability and therapeutic efficacy of phytochemicals.

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The authors declare that there are no conflicts of interest present.