danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2026-70-1-54-62dan-1294Research ArticleБИОЛОГИЯBIOLOGYБиосинтез каротиноидов красным дрожжевым грибом Rhodotorula glutinis БИМ Y-159Биосинтез каротиноидов красным дрожжевым грибом Rhodotorula glutinis БИМ Y-159https://orcid.org/0000-0002-8400-3182ШляхоткоЕ. А.ShlakhotkoE. A.

Шляхотко Екатерина Александровна – канд. биол. наук, ст. науч. сотрудник.

Ул. Купревича, 2, 220084, Минск

2, Kuprevich Str., 220084, Minsk

ekaterina_shl@mail.ruhttps://orcid.org/0009-0005-4498-3912СапуноваЛ. И.SapunovaL. I.

Сапунова Леонида Ивановна – канд. биол. наук, доцент, заведующий лабораторией.

Ул. Купревича, 2, 220084, Минск

2, Kuprevich Str., 220084, Minsk

leonida@mbio.bas-net.byЛобанокА. Г.LobanokA. G.

Лобанок Анатолий Георгиевич – академик, д-р биол. наук, гл. науч. сотрудник.

Ул. Купревича, 2, 220084, Минск

2, Kuprevich Str., 220084, Minsk

aglobanok@gmail.comhttps://orcid.org/0000-0002-5650-8538ШабуняП. С.ShabunyaP. S.

Шабуня Полина Станиславовна – канд. биол. наук, ст. науч. сотрудник.

Ул. Купревича, 5/2, 220084, Минск

5/2, Kuprevich Str., 220084, Minsk

pshabunya@rambler.ruИнститут микробиологии Национальной академии наук БеларусиInstitute of Microbiology of the National Academy of Sciences of BelarusИнститут биоорганической химии Национальной академии наук БеларусиInstitute of Bioorganic Chemistry of the National Academy of Sciences of Belarus2026020320267015462Copyright © Шляхотко Е.А., Сапунова Л.И., Лобанок А.Г., Шабуня П.С., 20262026Шляхотко Е.А., Сапунова Л.И., Лобанок А.Г., Шабуня П.С.Shlakhotko E.A., Sapunova L.I., Lobanok A.G., Shabunya P.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1294

Исследованы параметры биосинтеза каротиноидов у штамма Rhodotorula glutinis БИМ Y-159, установлен состав и соотношение отдельных пигментов. Показано, что максимальный уровень продукции каротиноидов (5,7 мг/г биомассы) достигается в условиях глубинного культивирования дрожжевого гриба в среде с высоким содержанием углеводов (глюкоза, 11 %) и дефицитом азотного питания. Ионы Cu2+ в концентрации 0,5 мМ стимулируют продукцию каротиноидов у R. glutinis БИМ Y-159 на 17,5–17,8 %. В условиях глубинного культивирования штамма состав комплекса продуцируемых каротиноидов представлен торуленом, торулородином, ζи β-каротинами, а также не идентифицированным компонентом. На долю β-каротина, торулина и торулородина приходится соответственно 16, 40 и 32 % синтезированных R. glutinis БИМ Y-159 каротиноидов.

Carotenogenesis, composition and ratio of individual carotenoid components synthesized by Rhodotorula glutinis BIM Y-159 was investigated. It was found that peak level of carotenoid production (5.7 mg/g biomass) was reached during submerged fermentation of yeast in media with high concentration of carbohydrates (glucose 11 %) and deficiency of nitrogen nutrition. Cu2+ ions in concentration 0.5 mM stimulated production of carotenoids in R. glutinis BIM Y-159 by 17.5–17.8 %. Under optimal conditions the produced carotenoid complex is represented by torulene, torulorhodin, ζand β-carotenes plus an unidentified component. β-Carotene, torulene, and torulorhodin amount for 16 %, 40 % and 32 % of carotenoids synthesized by R. glutinis BIM Y-159.

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The authors declare that there are no conflicts of interest present.