danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2026-70-4-313-319dan-1323Research ArticleБИОЛОГИЯBIOLOGYСтруктура конъюгата модифицированного пептида из активного центра нейраминидазы вируса гриппа А H1N1 с модифицированным капсидным белком вируса оспы обезьянStructure of a conjugate of a modified peptide from the active center of neuraminidase of the influenza A H1N1 virus with a modified capsid protein of the Monkeypox virushttps://orcid.org/0009-0006-0060-5351ТарасикМ. С.TarasikM. S.

Тарасик Марина Сергеевна – ст. преподаватель 

пр-т Дзержинского, 83, 220083, Минск 

Tarasik Marina S. – Senior Lecturer 

83, Dzerzhinskogo Ave., 220083, Minsk 

qetuo22@mail.ruhttps://orcid.org/0000-0003-1373-3329ХрусталёвВ. В.KhrustalevV. V.

Хрусталёв Владислав Викторович – д-р биол. наук, профессор, декан

ул. Курчатова, 10, 220030, Минск 

Khrustalev Vladislav V. – D. Sc. (Biology), Professor, Dean

10, Kurchatov Str., 220030, Minsk 

vvkhrustalev@mail.ruhttps://orcid.org/0000-0002-0060-4889ХрусталёваО. В.KhrustalevaO. V.

Хрусталёва Ольга Викторовна – канд. хим. наук, доцент

пр-т Дзержинского, 83, 220083, Минск 

Khrustaleva Olga V. – Ph. D. (Chemistry), Associate Professor

83, Dzerzhinskogo Ave., 220083, Minsk 

voi-olga@mail.ruhttps://orcid.org/0009-0008-8030-0009ХаритоноваУ. В.KharytonovaU. V.

Харитонова Ульяна Витальевна – аспирант 

ул. Курчатова, 10, 220030, Минск 

Kharytonova Ulyana V. – Postgraduate Student 

10, Kurchatov Str., 220030 

Ulyana221199@mail.ruhttps://orcid.org/0009-0009-5397-6799МатусевичП. В.MatusevichP. V.

Матусевич Павел Витальевич – научный сотрудник 

ул. Курчатова, 10, 220030, Минск 

Matusevich Pavel V. – Researcher 

10, Kurchatov Str., 220030 

matpavit@gmail.comhttps://orcid.org/0000-0001-9430-8492СемакИ. В.SemakI. V.

Семак Игорь Викторович – канд. биол. наук, доцент, заведующий кафедрой

ул. Курчатова, 10, 220030, Минск 

Semak Igor V. – Ph. D. (Biology), Associate Professor, Head of the Department

10, Kurchatov Str., 220030 

semak@bsu.byhttps://orcid.org/0009-0000-5660-6536МалюшковаЕ. В.MaliushkovaE. V.

Малюшкова Елена Викторовна – ст. науч. сотрудник, ст. преподаватель

ул. Курчатова, 10, 220030, Минск 

Maliushkova Elena V. – Senior Researcher, Senior Lecturer

10, Kurchatov Str., 220030 

malyushkova@gmail.comhttps://orcid.org/0000-0002-7888-4518КорикЕ. О.KorikE. O.

Корик Елена Олеговна – канд. биол. наук, доцент 

ул. Курчатова, 10, 220030, Минск 

Korik Elena O. – Ph. D. (Biology), Associate Professor

10, Kurchatov Str., 220030 

elkorik@gmail.comhttps://orcid.org/0000-0002-7796-5316СтожаровА. Н.StojarovA. N.

Стожаров Александр Николаевич – д-р биол. наук, профессор

пр-т Дзержинского, 83, 220083, Минск 

Stojarov Aleksander N. – D. Sc. (Biology), Professor

83, Dzerzhinskogo Ave., 220083, Minsk 

stojarov@mail.ruШенЧенгуангShenChenguan

Ченгуанг Шен – профессор 

1023-1063, ул. Шатайнань, р-н Байюнь, Гуанчжоу 

Chenguan Shen – Professor

1023-1063, Shatai South Road, Baiyun District, Guangzhou 

5942342qq@163.comБелорусский государственный медицинский университетБеларусьBelarusian State Medical UniversityBelarusБелорусский государственный университетБеларусьBelarusian State UniversityBelarusЮжный медицинский университетКитайSouthern Medical UniversityChina202629082026704313319Copyright © Тарасик М.С., Хрусталёв В.В., Хрусталёва О.В., Харитонова У.В., Матусевич П.В., Семак И.В., Малюшкова Е.В., Корик Е.О., Стожаров А.Н., Шен Ч., 20262026Тарасик М.С., Хрусталёв В.В., Хрусталёва О.В., Харитонова У.В., Матусевич П.В., Семак И.В., Малюшкова Е.В., Корик Е.О., Стожаров А.Н., Шен Ч.Tarasik M.S., Khrustalev V.V., Khrustaleva O.V., Kharytonova U.V., Matusevich P.V., Semak I.V., Maliushkova E.V., Korik E.O., Stojarov A.N., Shen C.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/1323

По результатам спектрального анализа оригинального вакцинного антигена (конъюгата модифицированного белка капсида A29L вируса оспы обезьян и одиннадцати молекул модифицированного пептида активного центра нейраминидазы вируса гриппа А H1N1) установлено, что пептидные фрагменты формируют бета-шпильки. Взаимодействие этих фрагментов приводит к образованию межмолекулярной параллельной и антипараллельной бета-структуры, стабильной в температурном интервале от 5 до 75 °С.

Spectral analysis of the original vaccine antigen (a conjugate of a modified Monkeypox virus A29L capsid protein and eleven molecules of a modified active-site peptide from the influenza A H1N1 virus neuraminidase) revealed that the peptide fragments form beta-hairpins. The interaction of these fragments results in the formation of an intermolecular parallel and antiparallel beta-sheet structure, stable in the temperature range of 5 to 75 °C.

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The authors declare that there are no conflicts of interest present.