danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"dan-23Research ArticleАГРАРНЫЕ НАУКИAGRARIAN SCIENCESПОЛУЧЕНИЕ ООЦИТОВ КОРОВ ПУТЕМ ТРАНСВАГИНАЛЬНОЙ ПУНКЦИИ ФОЛЛИКУЛОВCOW OOCYTE PICK-UP BY THE TRANSVAGINAL PUNCTURE OF FOLLICLESПЕСТИСВ. К.PESTISV. K.

член-корреспондент

ggaubio@mail.ruГОЛУБЕЦЛ. В.GOLUBETSL. V.ggau@ggau.byДЕШКОА. С.DESHKOA. S.deshkoas@mail.ruКЫССАИ. С.KYSSAI. S.semexbelarus@tut.byПОПОВМ. В.POPOVM. V.maxim.popov10@mail.ruГродненский государственный аграрный университет, ГродноGrodno State Agrarian University, GrodnoОАО «Почапово», Пинск«Pochapovo», Pinsk201622052016601123128Copyright © ПЕСТИС В.К., ГОЛУБЕЦ Л.В., ДЕШКО А.С., КЫССА И.С., ПОПОВ М.В., 20162016ПЕСТИС В.К., ГОЛУБЕЦ Л.В., ДЕШКО А.С., КЫССА И.С., ПОПОВ М.В.PESTIS V.K., GOLUBETS L.V., DESHKO A.S., KYSSA I.S., POPOV M.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/23

В работе представлены результаты изучения влияния некоторых факторов на эффективность получения ооцитов крупного рогатого скота путем трансвагинальной пункции фолликулов. Установлено, что уровень извлекаемости ооцит-кумулюсных комплексов от числа проаспирированных фолликулов составляет 68,7 %. Выход ооцитов на одну аспирацию – 2,7, в том числе на одну положительную по извлечению – 3,3. Наиболее приемлемой для аспирации является игла диаметром 17G независимо от уровня вакуума. Наибольшее количество аспирированных фолликулов (6,2 и 8,0) и полученных ооцитов (4,0 и 5,5) отмечено при использовании для стимуляции фолликулостимулирующего гормона Плюсет в дозе 750 И. Е. как без, так и с использованием в схеме стимуляции прогестагенного препарата Crestar.

The results of studying the influence of some factors on the efficiency of the cattle pick-up are presented in the article. It is established that the level of recoverability of the oocyte-cumulus complexes from the number of punctured follicles is 68.7%. The yield of oocytes per one aspiration is 2.7, including per one positive aspiration for extraction – 3.3. Most acceptable for aspiration is the 17G dia needle regardless of the vacuum level. The greatest number of aspirated follicles (6.2 and 8.0) and picked-up oocytes (4.0 to 5.5) is observed when for simulation the follicle-stimulating Pluset hormone is used at a dose of 750 I. E. with or without the use of the progestagen drug Crestar during the stimulation process.

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The authors declare that there are no conflicts of interest present.