danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"dan-37Research ArticleХИМИЯCHEMISTRYСВЯЗЫВАНИЕ ГЕЛЕВЫХ МАГНИТНЫХ МИКРОСФЕР С МОНОНУКЛЕАРНЫМИ КЛЕТКАМИ КРОВИBINDING OF MAGNETIC GEL MICROSPHERES TO BLOOD MONONUCLEAR CELLSЛАЗНЕВК. В.LAZNEVK. V.kvlaznev@mail.ruАГАБЕКОВВ. Е.AGABEKOVV. E.

академик

agabekov@ichnm.basnet.byИнститут химии новых материалов НАН Беларуси, МинскInstitute of Chemistry of New Materials of the National Academy of Sciences of Belarus, Minsk2016230520166026772Copyright © ЛАЗНЕВ К.В., АГАБЕКОВ В.Е., 20162016ЛАЗНЕВ К.В., АГАБЕКОВ В.Е.LAZNEV K.V., AGABEKOV V.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/37

Покрытие поливиниловым спиртом (ПВС) магнитных микросфер (ММС) на основе хитозана, сшитого глутаральдегидом, уменьшает их неспецифическое связывание с мононуклеарными клетками крови (МНК) эффективнее, чем покрытие карбоксиметилдекстраном со степенью карбоксилирования <10 %. Поливинилспиртовые ММС, покрытые дополнительным слоем ПВС после сшивки глутаральдегидом, в т. ч. функционализованные карбоксильными группами благодаря обработке H2O2, характеризуются низким неспецифическим связыванием с МНК. В осадке после магнитной сепарации на одну клетку приходится 4670 ± 760 поливинилспиртовых ММС, обработанных 4 % H2O2, а коммерческих ММС MagnosphereTM MS300/Carboxyl – 2040 ± 400.

A polyvinyl alcohol (PVA) coating decreases the non-specific binding of magnetic microspheres (MMS) composed of glutaraldehyde-cross-linked chitosan to blood mononuclear cells (BMC) more efficiently than a carboxymethyldextran (carboxylation degree <10 %) coating. PVA-composed MMS coated with an additional PVA layer after glutaraldehyde cross-linking, including ones functionalized with carboxyl groups due to the H2O2 treatment, demonstrate low non-specific binding to BMC. In the pellets resulting from magnetic separation, there were 4670 ± 760 PVA MMS and 2040 ± 400 MagnosphereTM MS300/Carboxyl commercial MMS per one BMC.

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The authors declare that there are no conflicts of interest present.