danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"dan-394Research ArticleМЕДИЦИНАMEDICINEНОВЫЕ ПОДХОДЫ В ДИАГНОСТИКЕ ОПУХОЛЕЙ ТОЛСТОЙ КИШКИ ПО КРИТЕРИЮ ДНК-ПЛОИДНОСТИNEW APPROACHES TO THE DIAGNOSIS OF SIGMOID COLON CANCER OF PATIENTS WITH COLORECTAL CANCER BY THE DNA-PLOIDY CRITERIONНиколаевичЛ. Н.NikolaevichL. N.

канд. биол. наук, доцент, заведующая лабораторией

Ph. D. (Biology), Assistant Professor, Head of the Laboratory

nikolarisa@tut.byЗалуцкийИ. В.ZalutskyI. V.

член-корреспондент, д-р мед. наук, профессор, директор

Corresponding Member, D. Sc. (Medicine), Professor, Director

biblio@fizio.bas-net.byРуденковаИ. В.RudenkovaI. V.

мл. науч. сотрудник

Junior researcher

kiranan4@gmail.comИнститут физиологии НАН БеларусиInstitute of Physiology of the National Academy of Sciences of Belarus2017020320176118588Copyright © Николаевич Л.Н., Залуцкий И.В., Руденкова И.В., 20172017Николаевич Л.Н., Залуцкий И.В., Руденкова И.В.Nikolaevich L.N., Zalutsky I.V., Rudenkova I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/394

Методом проточной цитометрии изучена ДНК-плоидность и молекулярно-биологические характеристики аденокарцином сигмовидной кишки. У 71  % пациентов выявлены диплоидные, а у 29  % – анеуплоидные опухоли. В диплоидном варианте опухоли при распределении клеток по стадиям клеточного цикла выявлены достоверные различия в содержании клеток в G0  / G1 и � S фазах клеточного цикла по сравнению с анеуплоидным вариантом опу- опухоли. Наблюдается повышенная ДНК – синтетическая и пролиферативная активность опухолевых клеток по сравнению с пациентами при анеуплоидном профиле опухоли. У пациентов с диплоидным профилем опухоли количество клеток в S фазе в 5 раз больше, нежели у больных с анеуплоидным вариантом опухоли. Можно предположить, что у больных анеуплоидным раком сигмовидной кишки частота рецидивов будет выше, а выживаемость ниже, чем при диплоидном профиле опухоли.

The DNA ploidy and molecular biological characteristics of adenocarcinoma of sigmoid colon by flow cytometry were studied. In 71  % of the patients, diploid was detected and in 29  % of the patients – aneuploid tumors. In the diploid tumor profile in the distribution of cell cycle phases, significant differences are revealed in the content of cells in G0 / G1 and S phases of the cell cycle, as compared to the aneuploid tumor profile. There is the increased DNA – the synthetic and proliferative activity of tumor cells compared to patients with the aneuploid tumor profile. Patients with the diploid tumor profile have a number of cells in the S phase 5 times more than in patients with the aneuploid tumor profile. It may be assumed that in patients with aneuploid sigmoid cancer, the recurrence rate is higher and the survival rate is lower than in those with the diploid tumor profile.

проточная цитометрияопухоли толстой кишкиплоидность клетокflow cytometrycolon tumorcell ploidyReferencesAssessment of clinically related outcomes and biomarker analysis for translational integration in colorectal cancer (ACROBATICC): study protocol for a population-based, consecutive cohort of surgically treated colorectal cancer and resected colorectal liver metastasis / S. Kjetil [et al.] // J. of Translational Medicine. – 2016. – Vol. 14. – P. 192–200. doi.org/10.1186/s12967-016-0951-4.Kjetil S., Watson M. M., Lea D., Nordgerd O., Arne S. J., Hagland R. H. Assessment of clinically related outcomes and biomarker analysis for translational integration in colorectal cancer (ACROBATICC): study protocol for a population-based, consecutive cohort of surgically treated colorectal cancer and resected colorectal liver metastasis. Journal of Translational Medicine, 2016, vol. 14, pp. 192–200. doi.org/10.1186/s12967-016-0951-4.DNA ploidy and morphology of colon tumors in the adenoma-carcinoma sequence / J. Staarmann [et al.] // Folia histochemica et cytobiologica. – 2015. – Vol. 53, N 1. – P. 11–18. doi.org/10.5603/fhc.a2015.0001.Staarmann J., Kotb W. F. A., Petersen I. DNA ploidy and morphology of colon tumors in the adenoma-carcinoma sequence. Folia histochemica et cytobiologica, 2015, vol. 53, no. 1, pp. 11–18. doi.org/10.5603/fhc.a2015.0001.Method for cancer diagnosis using DNA ploidy analysis with combination of fast Fourier transform and domain method / N. Hinata [et al.] // Transactions of Japanese Society for Medical and Biological Engineering. – 2014. – Vol. 52, N 3. – P. 136–144.Takeda S., Hinata N., Kanda H., Shioyama T., Suzuki A., Ishikawa Y., Yamaguchi T., Kato Y. Method for cancer diagnosis using DNA ploidy analysis with combination of fast Fourier transform and domain method. Transactions of Japanese Society for Medical and Biological Engineering, 2014, vol. 52, no. 3, pp. 136–144.Aneuploidy in health, disease, and aging / R. M. Ricke [et al.] // J. Cell Biol. – 2013. – Vol. 201, N 1. – P. 11–21. doi.org/10.1083/jcb.201301061.Ricke R. M., van Deursen J. M. Aneuploidy in health, disease, and aging. The Journal of  Cell  Biology, 2013, vol. 201, no. 1, pp. 11–21. doi.org/10.1083/jcb.201301061.A study on the method for cancer diagnosis using FFT on DNA ploidy analysis / N. Hinata [et al.] // Transactions of Japanese Society for Medical and Biological Engineering. – 2013. – Vol. 51, N 2. – P. 103–111.Hinata N., Kanda H., Shioyama T., Suzuki A., Takeda S., Ishikawa Y., Yamaguchi T., Kato Y. A study on the method for cancer diagnosis using FFT on DNA ploidy analysis. Transactions of Japanese Society for Medical and Biological Engineering, 2013, vol. 51, no. 2, pp. 103–111.The consequences of chromosomal aneuploidy on the transcriptome of cancer cells / T. Ried [et al.] // Biochim. Biophys. Acta. – 2012. – Vol. 1819, N 7. – P. 784–793. doi.org/10.1016/j.bbagrm.2012.02.020.Ried T., Hu Y., Difilippantonio M. J., Ghadimi B. M., Grade M., Camps J. The consequences of chromosomal aneuploidy on the transcriptome of cancer cells. Biochimica et Biophysica  Acta  (BBA) - Gene Regulatory Mechanisms, 2012, vol. 1819, no. 7, pp. 784–793. doi.org/10.1016/j.bbagrm.2012.02.020.

The authors declare that there are no conflicts of interest present.