danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"dan-447Research ArticleТЕХНИЧЕСКИЕ НАУКИTECHNICAL SCIENCESМАГНИТОФОРЕЗ И ОСОБЕННОСТИ КИСЛОРОДНОГО ОБМЕНА В РАЗБАВЛЕННОЙ СУСПЕНЗИИ ЭРИТРОЦИТОВMAGNITOPHORESIS AND OXYGEN EXCHANGE IN DILUTE SUSPENSION OF ERYTHROCYTESКашевскийБ. Э.KashevskyB. E.ЖолудьА. М.ZholudA. M.КашевскийС. Б.KashevskyS. B.Институт тепло- и массообмена имени А. В. Лыкова Национальной академии наук БеларусиA. V. Luikov Heat and Mass Transfer Institute of the National Academy of Sciences of Belarus201705102017614103109Copyright © Кашевский Б.Э., Жолудь А.М., Кашевский С.Б., 20172017Кашевский Б.Э., Жолудь А.М., Кашевский С.Б.Kashevsky B.E., Zholud A.M., Kashevsky S.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/447

Особенности кислородного обмена отдельных эритроцитов изучены с помощью метода их магнитофоретической магнитометрии, основанного на регистрации траекторий движения клеток в микрожидкостной ячейке Хеле–Шоу при воздействии высокоградиентного магнитного поля. Впервые установлено, что в покоящейся разбавленной суспензии эритроцитов при атмосферном парциальном давлении кислорода степень оксигенации клеточного гемоглобина понижена в 4 раза по сравнению со стандартной оксигенацией гемоглобина крови. При этом приложение импульса сдвигового течения приводит к скачкообразному увеличению оксигенации до стандартного уровня, а восстановление низкого равновесного уровня оксигенации клеточного гемоглобина после прекращения течения происходит за время, измеряемое минутами. Полученные результаты выявляют новые особенности функционирования эритроцитов и могут найти применение для гематологической диагностики.

 

 

 

 

Abstract

Abstract. Oxygen exchange in single erythrocytes is studied with the use of magnetophoretic magnetometry based upon the registration of cell trajectories in a microfluidic Hele-Shaw cell under the action of high-gradient magnetic field. It is found that, at atmospheric conditions, the equilibrium oxygenation degree of cellular hemoglobin is four times smaller as against a standard level of blood hemoglobin. Also, an application of shear flow leads to an instantaneous increase of cellular hemoglobin oxygenation up to a standard level, and it takes minutes for a low equilibrium level to be re-established. These findings give a new notion as upon erythrocyte functions and may be of use for the hematocrit diagnosis.

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The authors declare that there are no conflicts of interest present.