danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"dan-479Research ArticleМЕДИЦИНАMEDICINEПОЛИМОРФИЗМ ГЕНА РЕЦЕПТОРА ВИТАМИНА D И ПОКАЗАТЕЛИ ЭХОКАРДИОГРАФИИ У ЛИЦ С СЕРДЕЧНО-СОСУДИСТЫМИ ЗАБОЛЕВАНИЯМИPOLYMORPHISM OF THE VITAMIN D RECEPTOR GENE AND ECHOCARDIOGRAPHY INDICATORS IN PATIENTS WITH CARDIOVASCULAR DISEASESСнежицкийВ. А.SnezhitskiyVictor A.

член-корреспондент, д-р мед. наук, профессор, ректор

ул. Горько- го, 80, 230009

Corresponding Member, D. Sc. (Medicine), Professor, Rector

Gorkii Str., 80, 230009

snezh@grsmu.byЯнковскаяЛ. В.YankouskayaLudmila V.

канд. мед. наук, доцент, заведующий кафедрой

ул. Горького, 80, 230009

Ph. D. (Medicine), Assistant Professor, Head of the Department

Gorkii Str., 80, 230009

yankovliuda@yandex.ruСтепуроТ. Л.StepuroTatiana L.

канд. биол. наук, науч. сотрудник

ул. Горького, 80, 230009

Ph. D. (Biology), Researcher

Gorkii Str., 80, 230009

mikhno_t@yahoo.comНовогранВ. И.NovogranVictor I.

заведующий отделением

бул. Ленинского Комсомола, 59, 230030

Head of the Department

59, Leninskogo Komsomola Blvd., 230030

Гродненский государственный медицинский университет, ГродноGrodno State Medical University, GrodnoГородская клиническая больница № 3 г. Гродно, ГродноGrodno City Clinical Hospital № 3, Grodno20172101201861696105Copyright © Снежицкий В.А., Янковская Л.В., Степуро Т.Л., Новогран В.И., 20182018Снежицкий В.А., Янковская Л.В., Степуро Т.Л., Новогран В.И.Snezhitskiy V.A., Yankouskaya L.V., Stepuro T.L., Novogran V.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/479

Цель исследования – сравнение структурно-функциональных показателей миокарда при разных генотипах полиморфных маркеров BsmI (B/b) (rs1544410) и FokI (F/f) (rs2228570) гена рецептора витамина D (VDR) у лиц с сердечно-сосудистыми заболеваниями (ССЗ). Материалы и методы. Обследовано 198 пациентов с ССЗ. Определение BsmI и FokI гена VDR проводили методом полимеразной цепной реакции. Содержание в крови паратиреоидного гормона, 25(OH)D общего, эндотелина-1, активность ренина плазмы определяли методом иммунофер- ментного анализа. Определение в крови уровня кальция и фосфора проводилось колориметрическим методом. Эхо- кардиография выполнялась аппаратом GE Logic P5 Premium (Корея) фазированным секторным датчиком с частотой 2–4 мГц в режимах М-, В-, PW, CW. Результаты. Все обследованные были разделены на группы по генотипам FokI и BsmI гена VDR. При каждом полиморфизме группы были сопоставимы по возрасту, ССЗ, значениям артериального давления, частоте сердечных сокращений, индексу массы тела и уровням оцененных биомаркеров. Установлены достоверные (p < 0,05) отличия по размерам отделов аорты, левого предсердия (ЛП), размерам и объемам левого желудочка (ЛЖ) и его стенок, диаметром выходного тракта ЛЖ и фракцией выброса между группами с генотипом FF и ff. Кроме того, установлены отличия (p < 0,05) по размерам отделов аорты, ЛП и толщиной межжелудочковой перегородки между группами с генотипом ff и Ff. По генотипам BsmI гена VDR группы достоверно не отличались по оцененным структурно-функциональным показателям миокарда и аорты. Гипертрофия ЛЖ диагностирована у 78,6 % обследованных. Заключение. Полиморфизм FokI, но не BsmI гена VDR ассоциирован со структурно-функциональными показателями миокарда и аорты у лиц с ССЗ у жителей Гродненского региона Беларуси. С наибольшей частотой гипертрофия ЛЖ встречается при Ff (37,9 %) и Bb (33,8 %) генотипах. 

The aim of the study was to compare the structural and functional parameters of the myocardium in different genotypes of polymorphic markers BsmI (B/b) (rs1544410) and FokI (F/f) (rs2228570) of the vitamin D receptor gene (VDR) in individuals with cardiovascular diseases (CVD). Materials and Methods. We examined 198 patients with CVD. BsmI and FokI of the VDR gene were determined by the polymerase chain reaction. The blood levels of parathyroid hormone, 25(OH)D total, endothelin-1, plasma renin activity were revealed by the method of enzyme immunoassay. The calcium and phosphorus level in the blood was defined by the colorimetric method. Echocardiography was performed by GE Logic P5 Premium (Korea) with a phased sector sensor with a frequency of 2–4 MHz in the modes M-, B-, PW, CW. Results. All participants were divided into groups according to genotypes of FokI and BsmI of the VDR gene. For each polymorphism, the groups were comparable in age, CVD, blood pressure, heart rate, body mass index and levels of the estimated biomarkers. Significant differences (p < 0.05) in the sizes of the aorta and the left atrium (LA), in the sizes and volumes of the left ventricle (LV) and its walls, the diameter of the LV outlet tract and the ejection fraction between the groups with the genotypes of FF and ff were established. In addition, differences (p < 0.05) were found in the aorta size, LA and interventricular septum between the groups with the genotypes of ff and Ff. According to the genotypes of BsmI of the VDR gene, the groups did not differ significantly in the estimated structural and functional parameters of the myocardium and aorta. Hypertrophy of the LV is diagnosed in 78.6 % of participants. Conclusions. Polymorphism of FokI, but not of BsmI of the VDR gene is associated with structural and functional parameters of the myocardium and aorta in individuals with CVD in the Grodno region of Belarus. With the greatest frequency, LV hypertrophy occurs with Ff (37.9 %) and Bb (33.8 %).

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The authors declare that there are no conflicts of interest present.