danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2018-62-1-93-100dan-494Research ArticleМЕДИЦИНАMEDICINEРЕГУЛЯЦИЯ ПРОЛИФЕРАТИВНОЙ АКТИВНОСТИ И ХИМИОРЕЗИСТЕНТНОСТИ ОПУХОЛЕВЫХ КЛЕТОК АСКОРБАТОМ НАТРИЯREGULATION OF THE PROLIFERATIVE ACTIVITY AND CHEMORESISTANCE IN TUMOR CELLS BY SODIUM ASCORBATEМартиновичГ. Г.MartinovichGrigory G.

д-р биол. наук, доцент, заведующий кафедрой

пр. Независимости, 4, 220030, Минск

D. Sc. (Biology), Assistant Professor, Head of the Department

4, Nezavisimosti Ave., 220030, Minsk

martinovichgg@bsu.byМартиновичИ. В.MartinovichIrina V.

ст. науч. сотрудник

пр. Независимости, 4, 220030, Минск

Senior researcher

4, Nezavisimosti Ave., 220030, Minsk

parkun@bsu.byВчерашняяА. В.VcherashniayaAleksandra V.

мл. науч. сотрудник

пр. Независимости, 4, 220030, Минск

Junior researcher

4, Nezavisimosti Ave., 220030, Minsk

tuata_de_danann@mail.ruЗенковН. К.ZenkovNikolai K.

д-р биол. наук, вед. науч. сотрудник

ул. Академика Тимакова, 2, 630117, Новосибирск

D. Sc. (Biology), Leading researcher

2, Academician Timakov Str., 630117, Novosibirsk

lemen@centercem.ruМеньщиковаЕ. Б.MenshchikovaElena B.

д-р мед. наук, доцент, гл. науч. сотрудник

ул. Академика Тимакова, 2, 630117, Новосибирск

D. Sc. (Medicine), Assistant Professor, Chief researcher

2, Academician Timakov Str., 630117, Novosibirsk

lemen@centercem.ruЧеренкевичС. Н.CherenkevichSergei N.

академик, д-р биол. наук, профессор

пр. Независимости, 4, 220030, Минск

Academician, D. Sc. (Biology), Professor

4, Nezavisimosti Ave., 220030, Minsk

cherenkevich@bsu.byБелорусский государственный университетBelarusian State UniversityНаучно-исследовательский институт экспериментальной и клинической медициныResearch Institute of Experimental and Clinical Medicine20180503201862193100Copyright © Мартинович Г.Г., Мартинович И.В., Вчерашняя А.В., Зенков Н.К., Меньщикова Е.Б., Черенкевич С.Н., 20182018Мартинович Г.Г., Мартинович И.В., Вчерашняя А.В., Зенков Н.К., Меньщикова Е.Б., Черенкевич С.Н.Martinovich G.G., Martinovich I.V., Vcherashniaya A.V., Zenkov N.K., Menshchikova E.B., Cherenkevich S.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/494

Исследовано влияние аскорбата в физиологических концентрациях на пролиферативную активность и химиорезистентность клеток карциномы гортани человека линии НЕр-2. Установлено, что при действии аскорбата в концентрации 60 мкМ скорость пролиферации опухолевых клеток увеличивается в 1,5 раза. Аскорбат вызывает изменение функционального состояния опухолевых клеток, в результате которого увеличивается их резистентность к доксорубицину и тимохинону. Показано, что ингибитор НАДФН-оксидазы апоцинин блокирует стимулирующий эффект антиоксиданта. Полученные результаты свидетельствуют об участии активных форм кислорода, продуцируемых НАДФН-оксидазой, в механизме активации адаптационного ответа клеток, индуцированного аскорбатом. 

The effect of ascorbate in physiological concentrations on the proliferative activity and chemoresistance in human larynx carcinoma HEp-2 cells was studied. Ascorbate in a concentration of 60 μM was found to increase the cancer cells proliferation rate 1.5 times. Ascorbate changes the functional state of the cancer cells, thereby increasing their resistance to doxorubicin and thymoquinone. It was shown that apocynin (NADPH oxidase inhibitor) blocks the stimulating effect of the antioxidant. The results obtained suggest that reactive oxygen species produced by NADPH oxidase participate in the mechanism of cell adaptive response induced by ascorbate. 

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The authors declare that there are no conflicts of interest present.