danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2018-62-2-193-202dan-508Research ArticleБИОЛОГИЯBIOLOGYЗАВИСИМОСТЬ ЭКСПРЕССИИ ГЕНОВ, КОНТРОЛИРУЮЩИХ ФУНКЦИОНИРОВАНИЕ БРАССИНОСТЕРОИДОВ И ЭТИЛЕНА В КЛЕТКАХ РАСТЕНИЙ ТОМАТА, ОТ ГРАВИСТИМУЛЯЦИИ ИХ ВЕРХУШЕЧНЫХ ЛИСТЬЕВDEPENDENCE OF EXPRESSION OF GENES CONTROLLING BRASSINOSTEROIDS AND ETHYLENE ON GRAVISTIMULATION IN CELLS OF TOMATO APICAL LEAVESСуховееваС. В.SukhaveyevaS. V.

мл. науч. сотрудник

Junior researcher

suhoveevalmbc@mail.ruКабачевскаяЕ. М.КаbachevskayA. M.

канд. биол. наук, ст. науч. сотрудник

Ph. D. (Biology), Senior researcher

lmbc@ibp.ogr.byРадцевичН. А.RadtsevichN. A.

мл. науч. сотрудник

Junior researcher

natalliya.radtsevich@mail.ruВолотовскийИ. Д.VolotovskiI. D.

академик, д-р биол. наук, профессор, заведующий лабораторией

Academician, D. Sc. (Biology), Professor, Head of the Laboratory

volotovski@yahoo.comИнститут биофизики и клеточной инженерии Национальной академии наук Беларуси, МинскInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Minsk201820052018622193202Copyright © Суховеева С.В., Кабачевская Е.М., Радцевич Н.А., Волотовский И.Д., 20182018Суховеева С.В., Кабачевская Е.М., Радцевич Н.А., Волотовский И.Д.Sukhaveyeva S.V., Каbachevskay A.M., Radtsevich N.A., Volotovski I.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/508

Исследовали влияние гравистимуляции на изменение экспрессии генов в клетках листьев томата на ранних (15 мин – 3 ч) и поздних (более 3 ч – 24 ч) этапах гравитропического ответа. С использованием ОТ-ПЦР в режиме реального времени показана чувствительность к гравистимуляции экспрессии генов, ассоциированных с функционированием фитогормонов брассиностероидов и этилена. Предварительная обработка растений перед началом гравистимуляции этефоном (источником экзогенного этилена) приводила к увеличению относительного уровня экспрессии изученных генов в ответ на гравистимул.

 

The influence of gravistimulation on the gene expression level in tomato leaf cells at early (15 minutes – 3 hours) and late (more than 3 hours – 24 hours) stages of the gravitropic response was assessed. The sensitivity of expression of genes coding proteins associated with brassinosteroid and ethylene functioning was determined using real-time RT-PCR. The pre-treatment of tomato plants with ethephon (the source of exogenous ethylene) led to an increase in the relative level of expression of the investigated genes under gravistimulation in the plants.

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The authors declare that there are no conflicts of interest present.