danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2018-62-2-203-209dan-509Research ArticleБИОЛОГИЯBIOLOGYРОЛЬ ГЕНОВ RLM И AVRLM В РЕАЛИЗАЦИИ СПЕЦИФИЧЕСКОЙ УСТОЙЧИВОСТИ К ФОМОЗУ У РАПСАTHE ROLE OF RLM AND AVRLM GENES IN THE REALIZATION OF RAPE SPECIFIC RESISTANCE TO BLACKLEGЛемешВ. А.LemeshV. A.

канд. биол. наук, доцент, директор

Ph. D. (Biology), Assistant professor, Director

V.Lemesh@igc.byМозговаГ. В.MozgovaG. V.

канд. биол. наук, руководитель центра

Ph. D. (Biology), Head of the Centre

G.Mozgova@igc.byПилюкЯ. Э.PilyukYa. E.

канд. с.-х. наук, доцент, руководитель отдела

Ph. D. (Agrarian), Assistant professor, Head of the Department

iveya@list.ruХоружийН. Е.HoruzhiyN. E.

мл. науч. сотрудник

Junior researcher

nickolai_horuzhiy@mail.ruБыкЕ. С.BykE. S.

науч. сотрудник

Researcher

ekaterina-shapel@rambler.ruБураковаА. А.BurakovaA. A.

мл. науч. сотрудник

Junior researcher

A.Burakova@igc.byИнститут генетики и цитологии Национальной академии наук Беларуси, МинскInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus, MinskНаучно-практический центр Национальной академии наук Беларуси по земледелию, ЖодиноResearch and Practical Center of the National Academy of Sciences of Belarus for Arable Farming, Zhodino201820052018622203209Copyright © Лемеш В.А., Мозгова Г.В., Пилюк Я.Э., Хоружий Н.Е., Бык Е.С., Буракова А.А., 20182018Лемеш В.А., Мозгова Г.В., Пилюк Я.Э., Хоружий Н.Е., Бык Е.С., Буракова А.А.Lemesh V.A., Mozgova G.V., Pilyuk Y.E., Horuzhiy N.E., Byk E.S., Burakova A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/509

В ходе исследования выявлены ДНК-маркеры к местным расам L. maculans и установлено, что в популяции отобранного гриба присутствует последовательность AvrLm4-7, которая специфично распознается генами устойчивости Rlm4 и Rlm7. Выявлен SCAR-маркер BN204, позволяющий идентифицировать гомозиготные и гетерозиготные растения, несущие ген Rlm4. С использованием ДНК-маркера BN204 была проанализирована рабочая коллекция, включающая 22 сорта и 39 сортообразцов рапса. Результаты ПЦР позволили установить, что количество индивидуальных растений с геном устойчивости Rlm4 было выше, чем количество устойчивых форм, охарактеризованных путем заражения листовых эксплантов расами патогена, несущими последовательность AvrLm4-7. Предполагается, что данные индивидуальные растения содержат ген Rlm4, однако у них отсутствует ген Rlm7.

 

As part of the study, DNA markers for local races L. maculans were identified and found that the AvrLm4-7 sequence in the selected fungus population is specifically recognized by the resistance Rlm4 and Rlm7 genes. The SCAR marker BN204 was identified, which allows us to identify homozygous and heterozygous plants carrying the Rlm4 gene. The work collection, including 22 varieties and 39 hybrids of rape, was analyzed using the DNA marker BN204. PCR results allowed us to establish that the number of individual plants with the resistance Rlm4 gene was higher than the number of stable forms characterized by the infection of leaf explants with pathogen races carrying the AvrLm4-7 sequence. It is supposed that these individual plants contain the Rlm4 gene, but they lack the Rlm7 gene.

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The authors declare that there are no conflicts of interest present.