danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2018-62-4-439-446dan-538Research ArticleБИОЛОГИЯBIOLOGYХИМИЧЕСКИЙ СОСТАВ ЛЕТУЧИХ КОМПОНЕНТОВ В ЛИСТЬЯХ ЦИТРУСОВЫХ РАСТЕНИЙ, КУЛЬТИВИРУЕМЫХ В УСЛОВИЯХ ОРАНЖЕРЕИCHEMICAL COMPOSITION OF VOLATILE COMPONENTS IN THE LEAVES OF CITRUS PLANTS CULTIVATED UNDER GREENHOUSE CONDITIONSГеткоН. В.HetkaNelli V.

Гетко Нелли Владимировна – д-р биол. наук, доцент, заведующая лабораторией

ул. Сурганова, 2в, 220012, Минск

Hetka Nelli Vladimirovna – D. Sc. (Biology), Assistant Professor, Head of the Laboratory

2B, Surganov Str., 220012, Minsk

N.Hetko@cbg.org.byАлехнаА. И.AlehnaAnton I.

Алехна Антон Иванович – ст. науч. сотрудник

ул. Сурганова, 2в, 220012, Минск

Alehna Anton Ivanovic – Senior researcher

2B, Surganov Str., 220012, Minsk

anton.alekhna@gmail.comСубочВ. П.SubochVictor P.

Субоч Виктор Прокофьевич – канд. хим. наук, вед. науч. сотрудник

ул. Козлова, 29, 220037, Минск

Suboch Viktor Prokofievich – Ph. D. (Chemistry), Leading researcher

29, Kozlov Str., 220037, Minsk

SubochVP@mail.ruТитокВ. В.TitokVladimir V.

Титок Владимир Владимирович – член-корреспондент, д-р биол. наук, доцент, директор

ул. Сурганова, 2в, 220012, Минск

Titok Vladimir Vladimirovich – Corresponding Member, D. Sc. (Biology), Assistant Professor, Director

2B, Surganov Str., 220012, Minsk

V.Titok@cbg.org.byЦентральный ботанический сад Национальной академии наук БеларусиCentral Botanical Garden of the National Academy of Sciences of BelarusНаучно-практический центр Национальной академии наук Беларуси по продовольствиюScientific and Practical Foodstuffs Centre of the National Academy of Sciences of Belarus201813092018624439446Copyright © Гетко Н.В., Алехна А.И., Субоч В.П., Титок В.В., 20182018Гетко Н.В., Алехна А.И., Субоч В.П., Титок В.В.Hetka N.V., Alehna A.I., Suboch V.P., Titok V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/538

Исследован химический состав легко летучих компонентов листьев девяти таксонов, представляющих виды и разновидности рода цитрус (Citrus L.), которые культивируются в условиях оранжерей Центрального ботанического сада НАН Беларуси: C. unshiu (Tanaka ex Swingle) Marcow., C. grandis Osbeck cv. Bogatyr, C. sinensis Osbeck cv. Navel, C. medica L. cv. Sarcodactilis, C. medica cv. Павловский шишкан, Лимон Скерневицкий (’Ponderosa lemon’) – клон гибрида C. medica L. × C. limon (L.) Burm. f., Citrofortunella microcarpa, C. limon (L.) Osbeck cv. Павловский лимон, C. meyeri (Ju. Tanaka). Летучие компоненты извлекали с помощью твердофазного микроэкстрактора фирмы Supelco™, размещенного в паровоздушном пространстве над пробами воздушно сухих, мелко измельченных и нагретых до 40 °С образцов листьев. Анализ компонентного состава экстрактов осуществляли методом GC/MS c использованием системы Agilent Technologies 6850 Series II (Network GC System/5975B (VL MSD)). В листьях исследуемых цитрусовых было выявлено 36 компонентов: монотерпены (14), сесквитерпены (11), а также кислородсодержащие соединения: терпеновые спирты, альдегиды, эфиры (11). Как показали исследования, циклические углеводороды монотерпен (Z)-β-trans-Ocimene и 3 сесквитерпена (β-caryophyllene, (3E,6E)-α-farnesene и α-caryophyllene) выявлены в составе летучих компонентов листьев у всей исследуемой группы цитрусовых. При этом, 2 сесквитерпена: (3E,6E)-α-farnesene и α-caryophyllene, исходя из их долей в общем объеме летучих соединений, являются для большинства таксонов доминантными. Сесквитерпен beta-Elemene присутствует в качестве доминантного компонента исключительно у видов и разновидностей, представляющих так называемую группу сладких цитрусовых: C. unshiu, C. grandis cv. Bogatyr, C. sinensis× cv. Navel, C. meyeri, что позволяет рассматривать его в качестве значимого признака в таксономии рода Citrus L.

The article presents the results of research of the chemical composition of easily volatile components in the leaves of nine taxa representing species and varieties of the genus Citrus L. cultivated under the conditions of greenhouses of the Central Botanical Garden of the National Academy of Sciences of Belarus as: C. unshiu (Tanaka ex Swingle) Marcow., C. grandis Osbeck cv. Bogatyr, C. sinensis Osbeck cv. Washington Navel, C. medica L. cv. Sarcodactilis, C. medica cv. Pavlovskiy Shishkan, Lemon Skernevitsky (’Ponderosa lemon’) is a clone of the hybrid C. medica L. × C. limon (L.) Burm. f., Citrofortunella microcarpa, C. limon (L.) Osbeck cv. Pavlovskiy, C.meyeri (Ju. Tanaka). The volatile components were extracted using a Supelco™ solid-phase microextractor placed in the vapour-air space above air-dried, finely ground and heated to 40 °C leaf samples. The component composition of the extracts was analyzed by the GC/MS method using the Agilent Technologies 6850 Series II system (Network GC System/5975B (VL MSD). Total 36 components were identified in the leaves of citrus plants: monoterpenes (14), sesquiterpenes (11), and oxygen-containing compounds: terpene alcohols, aldehydes, ethers (11). The studies have shown that the cyclic hydrocarbons monoterpene (Z)-β-trans-Ocimene and 3 sesquiterpenes (β-caryophyllene, (3E, 6E)-α-farnesene, and α-caryophyllene) were detected in the volatile components in leaves in the whole citrus group. While 2 sesquiterpenes: (3E,6E)-α-farnesene and α-caryophyllene are dominant for the most taxa based on their proportions in the total volume of volatile compounds. Sesquiterpene beta-Elemene is present as a dominant component exclusively in species and varieties representing the so-called “sweet citrus” group as it follows from the results of the studies: C. unshiu, C. grandis cv. Bogatyr, C. sinensis× cv. Washington Navel, C. meyeri, which allows one to consider it as a significant feature in the taxonomy of the genus Citrus L.

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The authors declare that there are no conflicts of interest present.