danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2018-62-4-447-454dan-539Research ArticleБИОЛОГИЯBIOLOGYАНАЛИЗ ГИНЗЕНОЗИДОВ В КОРНЯХ ЖЕНЬШЕНЯ НАСТОЯЩЕГО (PANAX GINSENG), ИНТРОДУЦИРОВАННОГО В ЦЕНТРАЛЬНОМ БОТАНИЧЕСКОМ САДУ НАН БЕЛАРУСИANALYSIS OF GYNZENOSIDES IN THE ROOTS OF PANAX GINSENG INTRODUCED IN THE CENTRAL BOTANICAL GARDEN OF NAS OF BELARUSКочкинД. В.KochkinDmitry V.

Кочкин Дмитрий Владимирович – канд. биол. наук, ст. науч. сотрудник

Ленинские горы, 1/12, 119234, Москва

Kochkin Dmitry Vladimirovich – Ph. D. (Biology), Senior researcher

1/12, Leninskie gory, 119234, Moscow

dmitrykochkin@mail.ruГлаголеваЕ. С.GlagolevaElena S.

Глаголева Елена Сергеевна – науч. сотрудник

Ленинские горы, 1/12, 119234, Москва

Glagoleva Elena Sergeevna – Researcher

1/12, Leninskie gory, 119234, Moscow

_yuga@mail.ruГалишевБ. А.GalischevBoris A.

Галишев Борис Александрович – инженер-исследователь

ул. Куйбышева, 48, 620083, Екатеринбург

Galishev Boris Aleksandrovich – Engineer-researcher

48, Kuibyshev Str., 620083, Yekaterinburg

galishev@mail.ruСпиридовичЕ. В.SpiridovichElena V.

Спиридович Елена Владимировна – канд. биол. наук, доцент, заведующая лабораторией

ул. Сурганова, 2В, 220012, Минск

Spiridovich Elena Vladimirovna – Ph. D. (Biology), Assistant Professor, Head of the Laboratory

2B, Surganov Str., 220012, Minsk

e.spiridovich@cbg.org.byНосовА. М.NosovAlexander M.

Носов Александр Михайлович – д-р биол. наук, профессор, заведующий кафедрой

Ленинские горы, 1/12, 119234, Москва

Nosov Alexander Mikhailovich – D. Sc. (Biology), Professor, Head of the Department

1/12, Leninskie gory, 119234, Moscow

al_nosov@mail.ruРешетниковВ. Н.ReshetnikovVladimir N.

Решетников Владимир Николаевич – академик, д-р биол. наук, профессор, заведующий отделом

ул. Сурганова, 2В, 220012, Минск

Reshetnikov Vladimir Nikolaevich – Academician, D. Sc. (Biology), Professor, Head of the Department

2B, Surganov Str., 220012, Minsk

v.reshetnikov@cbg.org.byМосковский государственный университет имени М. В. ЛомоносоваLomonosov Moscow State UniversityУральский федеральный университет имени первого Президента России Б. Н. ЕльцинаUral Federal University named after the first President of Russia B. N. YeltsinЦентральный ботанический сад Национальной академии наук БеларусиCentral Botanical Garden of the National Academy of Sciences of Belarus201813092018624447454Copyright © Кочкин Д.В., Глаголева Е.С., Галишев Б.А., Спиридович Е.В., Носов А.М., Решетников В.Н., 20182018Кочкин Д.В., Глаголева Е.С., Галишев Б.А., Спиридович Е.В., Носов А.М., Решетников В.Н.Kochkin D.V., Glagoleva E.S., Galischev B.A., Spiridovich E.V., Nosov A.M., Reshetnikov V.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/539

Впервые с помощью высокоэффективной жидкостной хроматографии, совмещенной с массспектрометрией (ВЭЖХ-МС) проведено подробное изучение качественного и количественного состава гинзенозидов в корнях женьшеня настоящего (P. ginseng C. A. Mey.), интродуцированного в условиях Республики Беларусь (опытный участок ЦБС НАН Беларуси). Установлено, что в изученных корнях присутствуют все основные нейтральные гликозиды женьшеня (гинзенозиды Rb1, Rc, Rb2/Rb3, Rd, Rf, Rg1 и Re), а также их малонилированные производные (малонил-гинзенозиды Rb1, Rc, Rb2/Rb3, Rd, Rg1 и Re) и некоторые «минорные» гинзенозиды (20-глюко-гинзенозид Rf, нотогинзенозиды R1 и R2, изомеры малонил-гинзенозидов Rb1 и Rd). Показано также, что разные части корней P. ginseng существенно отличаются по суммарному содержанию гинзенозидов: для основного корня этот параметр составил 3,3 % от сухой массы, а для боковых корней – 7,8 % от сухой массы.

For the first time, a detailed study of the qualitative and quantitative composition of ginsenosides in the Panax ginseng roots was carried out with the help of high-performance liquid chromatography combined with mass spectrometry (HPLC-MS). The plants were introduced into the conditions of the Republic of Belarus at the experimental plot of the Central Botanical Garden of the National Academy of Sciences of Belarus. It was found that in the examined roots, all basic neutral glycosides of ginseng (ginsenosides Rb1, Rc, Rb2/Rb3, Rd, Rf, Rg1 and Re), as well as their malonylated derivatives (malonylginsenosides Rb1, Rc, Rb2/Rb3, Rd, Rg1 and Re) and some “minor” ginsenosides (20-gluco-ginsenoside Rf, notoginsenosides R1 and R2, isomers of malonyl-ginsenosides Rb1 and Rd) are present. The research also showed that different parts of the P. ginseng roots differ significantly in a total content of ginsenosides: for the main root, this parameter was 3.3 % of dry mass, and for the lateral roots – 7.8 % of dry mass.

интродукцияженьшеньАралиевыеPanax ginsengAraliaceaeтритерпеновые гликозидыгинзенозидыВЭЖХ-МСintroductionPanax ginsengAraliaceaetriterpene glycosidesginsenosidesHPLC-MSАвторы выражают признательность В. В. Титку – директору ЦБС НАН Беларуси, Л. В. Кухаревой – вед. науч. сотруднику, Т. В. Гиль – науч. сотруднику лаборатории биоразнообразия растительных ресурсов ЦБС НАН Беларуси за помощь и содействие в исследованиях. Работа выполнена при финансовой поддержке гранта Российского фонда фундаментальных исследований 16-54-00158 Бел_а и Белорусского республиканского фонда фундаментальных исследований № 16Р-083.The authors are grateful to V. V. Titko, Director of the Central Botanical Garden of NAS of Belarus, L. V. Kukhareva, leading researcher and Т. V. Gil, researcher of the Laboratory of Biodiversity of Plant Resources of the Central Botanical Garden of NAS of Belarus for help and assistance in research. 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The authors declare that there are no conflicts of interest present.