danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2018-62-6-712-718dan-571Research ArticleБИОЛОГИЯBIOLOGYКачество зерна у линий мягкой пшеницы T. aestivum с интрогрессией генетического материала T. dicoccoides и T. dicoccumGrain quality in bread wheat lines T. aestivum with introgression of genetic material T. dicoccoides and T. dicoccumhttps://orcid.org/0000-0002-1187-1317ОрловскаяО. А.OrlovskayaO. A.

канд. биол. наук, вед. науч. сотрудник

Ph. D. (Biology), Leading researcher

O.Orlovskaya@igc.byhttps://orcid.org/0000-0002-2242-7107ВакулаС. И.VakulaS. I.

канд. биол. наук, науч. сотрудник

Ph. D. (Biology). Researcher

s.vacula@igc.byhttps://orcid.org/0000-0003-0295-5022ХотылеваЛ. В.KhotylevaL. V.

академик, д-р биол. наук, профессор, гл. науч. сотрудник

Academician, D. Sc. (Biology), Professor. Chief researcher

L.Khotyleva@igc.byhttps://orcid.org/0000-0002-0175-9786КильчевскийА. В.KilchevskyA. V.

академик, д-р биол. наук, профессор, главный ученый секретарь

Academician, D. Sc. (Biology), Professor, Chief scientific secretary

Kilchev@presidium.bas-net.byИнститут генетики и цитологии Национальной академии наук Беларуси, МинскInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus, Minsk201813012019626712718Copyright © Орловская О.А., Вакула С.И., Хотылева Л.В., Кильчевский А.В., 20192019Орловская О.А., Вакула С.И., Хотылева Л.В., Кильчевский А.В.Orlovskaya O.A., Vakula S.I., Khotyleva L.V., Kilchevsky A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/571

Дикорастущие и культурные сородичи T. aestivum, которые являются источниками многих хозяйственно ценных генов, часто привлекаются для расширения генофонда пшеницы. Оценено влияние генетического материала тетраплоидных видов рода Triticum (T. dicoccum и T. dicoccoides) на качество зерна интрогрессивных линий яровой мягкой пшеницы. У линий с генетическим материалом T. dicoccum и T. dicoccoides в сравнении с родительскими формами проведена идентификация состава высокомолекулярных субъединиц глютенина, связанных с хлебопекарными свойствами зерна, с одновременной оценкой качества зерна по показателям: стекловидность, содержание белка, массовая доля и качество клейковины. Выделены линии с аллелями локусов Glu-1 от тетраплоидных сородичей пшеницы T. dicoccoides и Т. dicoccum. Установлено, что интрогрессия чужеродного генетического материала в геном мягкой пшеницы оказала положительный эффект на такие признаки качества зерна, как стекловидность, содержание белка и клейковины. Линии с аллелями локусов Glu-А1 от T. dicoccoides и Glu-В1 от Т. dicoccum были на уровне исходного сорта пшеницы или превосходили его по качеству клейковины. В результате проведенных исследований выделены новые линии яровой мягкой пшеницы с высоким качеством зерна, которые могут использоваться для селекции данной культуры.

Related wild and cultural wheat species are regularly involved for expanding T. aestivum genetic diversity because they contain many valuable genes. We evaluated the effect of the genetic material of tetraploid species of the genus Triticum (T. dicoccoides, T. dicoccum) on the grain quality of introgression lines of spring bread wheat. The composition of the high molecular weight glutenin subunits which play an essential role in the formation of wheat baking properties was identified in the introgression lines of bread wheat and their parental forms. The traits of grain quality (hardness, protein and gluten content, gluten quality) were also evaluated. The lines with Glu-1 loci alleles from wheat relatives T. dicoccoides and Т. dicoccum were selected. It was found that the introgression of alien genetic material into the common wheat genome had a positive effect on the parameters of grain quality such as hardness, protein and gluten content. The lines with Glu-A1 loci alleles from T. dicoccoides and Glu-B1 from T. dicoccum were at the level of a parent wheat variety or of a higher gluten quality. As a result of the research, the new lines of bread soft wheat with high grain quality were found and can be used in the crop breeding.

мягкая пшеницаT. dicoccumT. dicoccoidesвысокомолекулярные субъединицы глютенинаSDS-электрофорезкачество зернаcommon wheatT. dicoccumT. dicoccoideshigh molecular weight glutenin subunitsSDS-PAGEquality of grainReferencesТехнологические свойства зерна и муки у линий мягкой пшеницы с интрогрессией от Aegilops speltoides Tausch / Т. А. Пшеничникова [и др.] // Сельскохозяйственная биология. – 2007. – Т. 42, № 5. – С. 86–89.Pshenichnikova T. A., Ermakova M. F., Chistyakova A. k., Shchukina l. V., lapochkina I. o. Technological properties of grain and flour in soft wheat lines with introgression from Aegilops speltoides Tausch. Sel’skokhozyaistvennaya biologiya = Agricultural Biology, 2007, vol. 42, no. 5, pp. 86–89 (in Russian).Effects of an lr26 translocation on grain productivity and grain protein content in spring bread wheat / S. N. Sibikeev [et al.] // Ann. Wheat Newsletter. USA. – 2009. – Vol. 55. – P. 176.Sibikeev S. N., krupnova o. V., Voronina S. A., krupnov V. A., Druzhin A. E. Effects of an lr26 translocation on grain productivity and grain protein content in spring bread wheat. Annual Wheat Newsletter. USA, 2009, vol. 55, pp. 176.Characterization of HMW glutenin subunits in Thinopyrum intermedium, Th. bessarabicum, Lophopyrum elongatum, Aegilops markgrafii, and their addition lines in wheat / Z. X. Niu [et al.] // Crop Sci. – 2011. – Vol. 51, N 2. – P. 667–677. https://doi.org/10.2135/cropsci2010.04.0235Niu Z. X., klindworth R., Wang R. R.-C., Jauhar P. P., larkin P. J., Xu S. S. Characterization of HMW glutenin subunits in Thinopyrum intermedium, Th. bessarabicum, Lophopyrum elongatum, Aegilops markgrafii, and their addition lines in wheat. Crop Science, 2011, vol. 51, no. 2, pp. 667–677. https://doi.org/10.2135/cropsci2010.04.0235Analysis of dough rheological property and gluten quality characteristics in wild emmer wheat (Triticum dicoccoides (körn. ex Asch. et Graebn.) Schweinf.) / D. Zhang [et al.] // Genet. Resour. Crop Evol. – 2016. – Vol. 63, N 4. – P. 675–683. https://doi.org/10.1007/s10722-015-0275-xZhang D., yuan y., Su y., li S. Analysis of dough rheological property and gluten quality characteristics in wild emmer wheat (Triticum dicoccoides (körn. ex Asch. et Graebn.) Schweinf.). Genetic Resources and Crop Evolution, 2016, vol. 63, no. 4, pp. 675–683. https://doi.org/10.1007/s10722-015-0275-xХарактеристика колекцiйних зразкiв спельти (Triticum spelta l.) за елементами структури продуктивностi та хлибопекарською якiстю / В. В. Моргун [и др.] // Физиология растений и генетика. – 2016. – Т. 48, № 2. – С. 112–119.Morgun V. V., Sichkar S. M., Pochinok V. M., Ninieva A. k., Chugunkova T. V. Characterization of spelt collection samples (Triticum spelta l.) by elements of plant productivity structure and baking quality. Fiziologiya rasteniy i genetika = Plant Physiology and Genetics, 2016, vol. 48, no 2, pp. 112–119 (in Ukrainian).Singh, N. k. A simplified SDS-PAGE procedure for separating lMW subunits of glutenin / N. k. Singh, k. W. Shepherd, G. B. Cornish // J. Cereal Sci. – 1991. – Vol. 14, N 3. – P. 203–208. https://doi.org/10.1016/s0733-5210(09)80039-8Singh N. k., Shepherd k. W., Cornish G. B. A simplified SDS-PAGE procedure for separating lMW subunits of glutenin. Journal of Cereal Science, 1991, vol. 14, no. 3, pp. 203–208. https://doi.org/10.1016/s0733-5210(09)80039-8laemmli, U. k. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 / U. k. laemmli // Nature. – 1970. – Vol. 227, N 5259. – P. 680–685. https://doi.org/10.1038/227680a0laemmli U. k. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, vol. 227, no. 5259, pp. 680–685. https://doi.org/10.1038/227680a0Payne, P. I. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecularweight subunits of glutenin in hexaploid wheat / P. I. Payne, G. J. lawrence // Cereal Res. Com. – 1983. – Vol. 11, N 1. – P. 29–35.Payne P. I., lawrence G. J. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Research Communication, 1983, vol. 11, no. 1, pp. 29–35.levy, A. A. Increase in grain protein percentage in high-yielding common wheat breeding lines by genes from wild tetraploid wheat / A. A. levy, M. Feldman // Euphytica. – 1987. – Vol. 36, N 2. – P. 353–359. https://doi.org/10.1007/bf00041478levy A. A., Feldman M. Increase in grain protein percentage in high-yielding common wheat breeding lines by genes from wild tetraploid wheat. Euphytica, 1987, vol. 36, no. 2, pp. 353–359. https://doi.org/10.1007/bf00041478Nitrogen uptake and remobilization in tetraploid langdon durum wheat and a recombinant substitution line with the high grain protein gene Gpc-B1 / M. A. kade [et al.] // Plant Breeding. – 2005. – Vol. 124, N 4. – P. 343–349. https://doi. org/10.1111/j.1439-0523.2005.01110.xkade M. A., Barneix J., olmos S., Dubcovsky J. Nitrogen uptake and remobilization in tetraploid langdon durum wheat and a recombinant substitution line with the high grain protein gene Gpc-B1. Plant Breeding, 2005, vol. 124, no. 4, pp. 343–349. https://doi.org/10.1111/j.1439-0523.2005.01110.xКартирование локусов количественных признаков (QTl), ассоциированных с показателями качества зерна мягкой пшеницы, выращенного в различных условиях среды / Т. А. Пшеничникова [и др.] // Генетика. – 2008. – Т. 44, № 1. – С. 90–101.Pshenichnikova T. A., Ermakova M. F., Chistyakova A. k. Shchukina l. V., Berezovskaya E. V., lochwasser U., Röder M., Börner A. Mapping of the quantitative trait loci (QTl) associated with grain quality characteristics of the bread wheat grown under different environmental conditions. Russian Journal of Genetics, 2008, vol. 44, no. 1, pp. 74–84. https:// doi.org/10.1134/s1022795408010109one hundred years of grain omics: identifying the glutens that feed the world / M. Ribeiro [et al.] // Proteome Res. – 2013. – Vol. 12, N 11. – P. 4702–4716. https://doi.org/10.1021/pr400663tRibeiro M., Nunes-Miranda J. D., Branlard G., Carrillo J. M., Rodriguez-Quijano M., Igrejas G. one hundred years of grain omics: identifying the glutens that feed the world. Journal of Proteome Research, 2013, vol. 12, no. 11, pp. 4702– 4716. https://doi.org/10.1021/pr400663tEnvironmental factors of celiac disease: cytotoxicity of hulled wheat species Т. топососсит, T. turgidum l. ssp dicoccum and T. aestivum ssp. spelta / О. Vincentini [et al.] // J. Gastroenterol. Hepatol. – 2007. – Vol. 22, N 11. – P. 1816– 1822. https://doi.org/10.1111/j.1440-1746.2006.04680.xVincentini o., Maialetti F., Gazza l., Silano M., Dessì M., De Vincenzi M., Pogna N. E. Environmental factors of celiac disease: cytotoxicity of hulled wheat species Т. топососсит, T. turgidum l. ssp dicoccum and T. aestivum ssp. spelta. Journal of Gastroenterology and Hepatology, 2007, vol. 22, no. 11, pp. 1816–1822. https://doi.org/10.1111/j.1440- 1746.2006.04680.xMargiotta, B. Polymorphism of high Mr glutenin subunits in wild emmer Triticum turgidum subsp. dicoccoides: chromatographic, electrophoretic separation and PCR analysis of their encoding genes / B. Margiotta, G. Colaprico, M. Urbano // Genet. Resour. Crop Evol. – 2014. – Vol. 61, N 2. – P. 331–343. https://doi.org/10.1007/s10722-013-0037-6Margiotta B., Colaprico G., Urbano M. Polymorphism of high Mr glutenin subunits in wild emmer Triticum turgidum subsp. dicoccoides: chromatographic, electrophoretic separation and PCR analysis of their encoding genes. Genetic Resources and Crop Evolution, 2014, vol. 61, no. 2, pp. 331–343. https://doi.org/10.1007/s10722-013-0037-6Wheat seed storage proteins: advances in molecular genetics, diversity and breeding applications / A. Rasheed [et al.] // J. Cereal Sci. – 2014. – Vol. 60, N 1. – P. 11–24. https://doi.org/10.1016/j.jcs.2014.01.020Rasheed A., Xia X., yan yu., Appels R., Mahmood T., He Z. Wheat seed storage proteins: advances in molecular genetics, diversity and breeding applications. Journal of Cereal Science, 2014, vol. 60, no. 1, pp. 11–24. https://doi.org/10.1016/j. jcs.2014.01.020

The authors declare that there are no conflicts of interest present.