danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2019-63-1-61-71dan-586Research ArticleБИОЛОГИЯBIOLOGYСахарозависимая регуляция активности фосфолипазы D в проростках овсаSugar-dependent regulation of the phospholipase D activity in oat seedlingsКабачевскаяЕ. М.КаbachevskayaA. M.

Кабачевская Елена Михайловна - кандидат биологических наук, старший научный сотрудник.

Kabachevskaya Alena Mikhaylovna - Ph. D. (Biology), Senior researcher.

27, Ac-ademicheskaya Str., 220072, Minsk

lmbc@ibp.org.byСуховееваС. В.SukhaveyevaS. V.

Суховеева Светлана Владимировна - младший научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Sukhaveyeva Sviatlana Vladimirovna - Junior researcher.

27, Academicheskaya Str., 220072, Minsk

suhove-evalmbc@mail.ruВолотовскийИ. Д.VolotovskiI. D.

Волотовский Игорь Дмитриевич - академик, доктор биологических наук, профессор, заведующий лабораторией.

Ул. Академическая, 27, 220072, Минск

Volotovski Igor Dmitrievich - Academician, D. Sc. (Biology), Professor, Head of the Laboratory.

27, Academicheskaya Str., 220072, Minsk

volot@biobel.bas-net.byИнститут биофизики и клеточной инженерии, Национальная академия наук БеларусиInstitute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus2019120320196316171Copyright © Кабачевская Е.М., Суховеева С.В., Волотовский И.Д., 20192019Кабачевская Е.М., Суховеева С.В., Волотовский И.Д.Каbachevskaya A.M., Sukhaveyeva S.V., Volotovski I.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/586

Исследовали зависимость активности фосфолипазы D (ФлD), катализирующей катаболизм глице-рофосфолипидов, от действия экзогенных растворимых сахаров в условиях сахарного голодания растений, вызванного затемнением. Обнаружено, что активность ФлD в этиолированных проростках овса (Avena sativa L.) подавляется обработкой растений моно- и дисахаридами: глюкозой, сахарозой и маннозой. Эти же сахара нивелируют активирующее действие затемнения на активность фермента в зеленых проростках овса. Обработка проростков неорганическим фосфатом также приводит к модуляции активности данного фермента, сходной с действием сахаров. При этом действие протестированных сахаров имитирует световую модуляцию активности ФлD. Секвенирование частичной последовательности мРНК гена ФлD овса показывает более чем 80 %-ное сходство с последовательностями ФлDa других видов злаковых. Облучение этиолированных проростков овса красным светом и затемнение зеленых проростков ингибируют или индуцируют экспрессию выявленного гена ФлD соответственно. Таким образом, экспрессия гена ФлD овса и ферментативная активность продукта его экспрессии (ФлD) проявляют чувствительность к изменениям световых условий роста растений. Следовательно, эти данные указывают на то, что ФлD играет важную роль в адаптации растений к действию затемнения и связанного с ним сахарного голодания.

The dependence of the phospholipase D (PLD) activity, catalyzing the catabolism of glycerophospholipids, on exogenous soluble sugars under the conditions of sugar starvation caused by darkening of plants was studied in oat (Avena sativa L.) seedlings. It was found that the PLD activity in etiolated oat seedlings was suppressed by the treatment of plants with mono- and disaccharides: glucose, sucrose and mannose. These sugars neutralized the activating effect of darkening on the activity of PLD in green oat seedlings. The treatment of oat seedlings with inorganic phosphate also led to the modulation of the PLD activity similar to that with sugars. In this case, the action of the tested sugars mimicked the light modulation of the PLD activity. Sequencing the partial mRNA sequence of oat PLD gene showed more than 80 % homology with the sequences PlDαof other cereal species. Illumination of etiolated oat seedlings with red light and darkening of green seedlings in darkness inhibited or induced the PLD expression, respectively. Thus, the expression of PLD and the enzymatic activity of PLD showed the sensitivity to changes in the light conditions of a plant growth. Consequently, these data indicate that PLD plays an important role in adaptation of oat plants to darkening and sugar starvation.

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The authors declare that there are no conflicts of interest present.