danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2019-63-2-181-188dan-601Research ArticleБИОЛОГИЯBIOLOGYВыделение, характеристика и молекулярно-генетическая идентификация нового штамма бактерий Paenibacillus speciesScreening, characterization and molecular-genetical identification of a new bacterial strain Paenibacillus speciesСапуноваЛ. И.SapunovaL. I.

Сапунова Леонида Ивановна – канд. биол. наук, доцент, гл. науч. сотрудник.

ул. Купревича, 2, 220141, Минск.

Sapunova Leanida Ivanovna – Ph. D. (Biology), Chief researcher. 

2, Kuprevich Str., 220141, Minsk.

leonida@mbio.bas-net.byЛобанокА. Г.LobanokA. G.

Лобанок Анатолий Георгиевич – академик, д-р биол. наук, заведующий лабораторией. 

ул. Купревича, 2, 220141, Минск.

Lobanok Anatoli Georgievich – Academician, D. Sc. (Biology), Head of the Laboratory. 

2, Kuprevich Str., 220141, Minsk.

aglobanok@gmail.comЯцевичК. К.YatsevichK. K.

Яцевич Констанция Константиновна – науч. сотрудник. 

ул. Академическая, 27, 220027, Минск.

Yatsevich Kanstantsyia Kаnstantsynauna – Researcher.

27, Akademicheskaya Str., 220027, Minsk.

yakon-kon@yandex.ruКулишС. А.KulishS. A.

Кулиш Светлана Анатольевна – ст. науч. сотрудник. 

ул. Купревича, 2, 220141, Минск.

Kulish Sviatlana Anatolievna – Senior researcher. 

2, Kuprevich Str., 220141, Minsk.

kulish76@mail.ruТамковичИ. О.TamkovichI. A.

Тамкович Ирина Олеговна – канд. биол. наук, ст. науч. сотрудник. 

ул. Купревича, 2, 220141, Минск.

Tamkovich Iryna Alehauna – Ph. D. (Biology), Senior researcher. 

2, Kuprevich Str., 220141, Minsk.

irina-kazakevich@tut.byЕрховаЛ. В.YarkhovaL. V.

Ерхова Людмила Викторовна – мл. науч. сотрудник. 

ул. Купревича, 2, 220141, Минск.

Yarkhova Liudmila Viktarauna – Junior researcher.

2, Kuprevich Str., 220141, Minsk.

milka1288@mail.ruСысолятинЯ. Н.SysaliatsinYa. M.

Сысолятин Евгений Николаевич – мл. науч. сотрудник. 

ул. Академическая, 27, 220027, Минск.

Sysaliatsin Yauhen Mikalaevich – Junior researcher.

27, Akademicheskaya Str., 220027, Minsk.

meeugeny@yandex.ruИнститут микробиологии Национальной академии наук Беларуси.Institute of Microbiology of the National Aca demy of Sciences of Belarus.Институт генетики и цитологии Национальной академии наук Беларуси.Institute of Genetics and Cytology of the National Academy of Sciences of Belarus.201915052019632181188Copyright © Сапунова Л.И., Лобанок А.Г., Яцевич К.К., Кулиш С.А., Тамкович И.О., Ерхова Л.В., Сысолятин Я.Н., 20192019Сапунова Л.И., Лобанок А.Г., Яцевич К.К., Кулиш С.А., Тамкович И.О., Ерхова Л.В., Сысолятин Я.Н.Sapunova L.I., Lobanok A.G., Yatsevich K.K., Kulish S.A., Tamkovich I.A., Yarkhova L.V., Sysaliatsin Y.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/601

Из зерна пшеницы, контаминированного образующей полисахарид микрофлорой, выделена и охарактеризована культура бактерий ПС-К-17. Установлено, что изолят на агаризованных средах и в глубинной культуре со специфическими субстратами синтезирует бета-галактозидазу, амилазу, протеазу, пектиназу, целлюлазу, бета- глюканазу, липазу (эстеразу), альгиназу, а также внеклеточные полисахариды и пигменты, возможно, каротиноиды. На основании культурально-морфологических и физиолого-биохимических особенностей, а также филогенетического анализа нуклеотидных последовательностей гена 16S рРНК (код доступа MF443394 в GenBank) бактериальная культура идентифицирована как Paenibacillus species ПС-К-17. Исследуемый изолят образует одну филогенетическую ветвь с типовыми штаммами Paenibacillus nicotianae (98,3 %), Paenibacillus hordei (98,2 %), Paenibacillus kyungheensis (97,9 %), в пределах которой занимает обособленное положение. Штамм Paenibacillus sp. ПС-К-17 может найти применение в биотехнологии как продуцент внеклеточных полисахаридов и ферментов, расщепляющих растительные полимеры, а также как компонент микробного консорциума в составе новой кормовой добавки комплексного действия.

Bacterial variant PS-K-17 was isolated from wheat grain contaminated by polysaccharide-producing microbiota for further characterization. It was found that the isolate grown on agar slants and in submerged culture on media with specific substrates synthesized beta-galactosidase, amylase, protease, pectinase, cellulase, beta-glucanase, lipase (esterase), alginase, extracellular polysaccharides, and pigments, probably carotenoids. Based on cultural-morphological and physiological-biochemical properties and phylogenetic analysis of nucleotide sequences of 16S rRNA gene (access code MF443394 in GenBank) the bacterial culture was identified as Paenibacillus species PS-K-17. The studied isolate forms one phylogenetic branch with type strains Paenibacillus nicotianae (98.3 %), Paenibacillus hordei (98.2 %), Paenibacillus kyungheensis (97.9 %), holding wherein a separate position. Strain Paenibacillus sp. PS-K-17 may find use in biotechnology as a producer of extracellular polysaccharides and enzymes splitting plant polymeric substances as well as a component of microbial consortium-ingredient of a new complex feed additive.

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The authors declare that there are no conflicts of interest present.