danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2019-63-4-437-444dan-631Research ArticleХИМИЯCHEMISTRYВлияние комбинации цисплатина с брассиностероидами на рост раковых клетокEffect of combination of cisplatin with brassinosteroids on the growth of cancer cellsПанибратО. В.PanibratOlesya V.

Панибрат Олеся Владимировна – ст. науч. сотрудник

ул. Купревича, 5/2, 220141, Минск

Panibrat Olesya Vladimirovna – Senior researcher

5/2, kuprevich Str., 220141, Minsk

panibrat@iboch.byЖабинскийВ. Н.ZhabinskiiVladimir N.

Жабинский Владимир Николаевич – член-корреспондент, д-р хим. наук, доцент, гл. науч. сотрудник, заместитель заведующего лабораторией

ул. Купревича, 5/2, 220141, Минск

Zhabinskii Vladimir Nikolaevich – Corresponding Member, D. Sc. (Chemistry), Assistant professor, Chief researcher, Deputy Head of the Laboratory

5/2, kuprevich Str., 220141, Minsk

vz@iboch.byХрипачВ. А.KhripachVladimir A.

Хрипач Владимир Александрович – академик, д-р хим. наук, профессор, заведующий лабораторией

ул. Купревича, 5/2, 220141, Минск

Khripach Vladimir Alexandrovich – Academician, D. Sc. (Chemistry), Professor, Head of the Laboratory

5/2, kuprevich Str., 220141, Minsk

khripach@iboch.byИнститут биоорганической химии Национальной академии наук БеларусиInstitute of Bioorganic Chemistry of the National Academy of Sciences of Belarus201913092019634437444Copyright © Панибрат О.В., Жабинский В.Н., Хрипач В.А., 20192019Панибрат О.В., Жабинский В.Н., Хрипач В.А.Panibrat O.V., Zhabinskii V.N., Khripach V.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/631

Изучено влияние природных брассиностероидов 24-эпибрассинолида и 28-гомокастастерона, а также их синтетических аналогов (22S,23S)-24-эпибрассинолида и (22S,23S)-28-гомокастастерона на противоопухолевую активность классического цитостатика цисплатина на опухолевых линиях A549 (карцинома легких человека) и Hep G2 (карцинома печени человека). Все четыре соединения в сочетании с цисплатином ингибировали рост раковых клеток. Более эффективными были комбинации с низкими концентрациями синтетических брассиностероидов. При концентрации брассиностероидов в 1 мкМ IC50 цисплатина уменьшалась почти в 2 раза. Полученные результаты свидетельствуют о возможности снижения эффективных доз классических противоопухолевых препаратов путем их использования в комбинации с брассиностероидами, что способствовало бы смягчению негативных последствий химиотерапии.

In this work, the effect of brassinosteroids on the antitumor activity of classical cytostatic cisplatin in tumor cell lines A549 (human lung carcinoma) and Hep G2 (human hepatocellular carcinoma) was evaluated. Natural brassinosteroids 24-epibrassinolide and 28-homocastasterone, as well as their synthetic analogues (22S,23S)-24-epibrassinolide and (22S,23S)-28-homocastasterone were used. All four compounds with cisplatin inhibited the growth of cancer cells more effectively than cisplatin alone. Combinations with low concentrations of synthetic brassinosteroids were more effecient, and at 1 µM decreased the IC50 of cisplatin by almost 2 times. The results suggest a possible benefit of combinations of classical antitumor drugs with brassinosteroids in overcoming the negative effects of chemotherapy by reducing their effective doses.

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The authors declare that there are no conflicts of interest present.