danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2019-63-6-730-735dan-834Research ArticleБИОЛОГИЯBIOLOGYВзаимодействие активных форм кислорода с галлоцианином при активации нейтрофиловInteraction between reactive oxygen species and gallocyanine under neutrophil activationЛуценкоВ. Е.LutsenkoV. E.

Луценко Вероника Евгеньевна – аспирант. 

пр. Независимости, 4, 220030, Минск

Lutsenko Veronika Evgenievna – Postgraduate student. 

4, Nezavisimosti Ave., 220030, Minsk

ГригорьеваД. В.GrigorievaD. V.

Григорьева Дарья Владимировна – канд. биол. наук, ст. науч. сотрудник. 

пр. Независимости, 4, 220030, Минск

Grigorieva Daria Vladimirovna – Ph. D. (Biology), Senior researcher. 

4, Nezavisimosti Ave., 220030, Minsk

nika.lutsenko@tut.byГорудкоИ. В.GorudkoI. V.

Горудко Ирина Владимировна – канд. биол. наук, доцент. 

пр. Независимости, 4, 220030, Минск

Gorudko Irina Vladimirovna – Ph. D. (Biology), Associate professor. 

4, Nezavisimosti Ave., 220030, Minsk

СоколовА. В.SokolovA. V.

Соколов Алексей Викторович – д-р биол. наук, профессор, заведующий лабораторией. 

ул. Академика Павлова, 12, 197376, Санкт-Петербург

Sokolov Alexey Viktorovich – D. Sc. (Biology), Professor, Head of the Laboratory.

12, Academician Pavlov Str., 197376, Saint Petersburg

biochemsokolov@gmail.comПанасенкоО. М.PanasenkoO. M.

Панасенко Олег Михайлович – д-р биол. наук, профессор, заведующий отделом. 

ул. Малая Пироговская, 1а, 119435, Москва

Panasenko Oleg Mikhailovich – D. Sc. (Biology), Professor, Head of Department. 

1а, Malaya Pirogovskaya Str., 119435, Moscow

ЧеренкевичС. Н.CherenkevichS. N.

Черенкевич Сергей Николаевич – академик, д-р биол. наук, профессор. 

пр. Независимости, 4, 220030, Минск

Cherenkevich Sergey Nikolaevich – Academician, D. Sc. (Biology), Professor. 

4, Nezavisimosti Ave., 220030, Minsk

Белорусский государственный университетBelarusian State UniversityИнститут экспериментальной медициныInstitute of Experimental MedicineФедеральный научно-клинический центр физико-химической медицины Федерального медико-биологического агентстваFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency201905012020636730735Copyright © Луценко В.Е., Григорьева Д.В., Горудко И.В., Соколов А.В., Панасенко О.М., Черенкевич С.Н., 20202020Луценко В.Е., Григорьева Д.В., Горудко И.В., Соколов А.В., Панасенко О.М., Черенкевич С.Н.Lutsenko V.E., Grigorieva D.V., Gorudko I.V., Sokolov A.V., Panasenko O.M., Cherenkevich S.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/834

В работе изучены химические изменения красителя галлоцианина при его взаимодействии c активными формами кислорода (•О2ˉ, H2O2) и хлорноватистой кислотой (HOCl). Показано, что данный краситель в растворе претерпевает химические превращения как при действии активных форм кислорода, так и галогенов. Согласно полученным данным, в суспензии активированных нейтрофилов основной вклад в превращение красителя вносит •О2ˉ. Следовательно, галлоцианин может быть применен для оценки функциональной активности нейтрофилов, а именно НАДФН-оксидазного комплекса, а также при разработке терапевтических методов лечения с применением антиоксидантов при заболеваниях, ассоциированных с развитием окислительного стресса.

Reactive oxygen species (ROS) have a crucial role in human physiological and pathophysiological processes. Prolonged exposure to high ROS concentrations may lead to cardiovascular, neurodegenerative, etc. diseases. In this study, gallocyanine has been proposed to register the ROS production. The gallocyanine spectral properties changes under ROS (•О2ˉ, H2O2) and reactive halogen (HOCl) species are analyzed. It is shown that the dye is oxidized in solution, both under the action of ROS and reactive halogen species. Based on the data obtained, it is possible to suggest that superoxide anion radicals make a major contribution to chemical conversion of the dye in suspensions of activated neutrophils. It is that gallocyanine can be used to assess the functional activity of neutrophils, namely, the NADPH-oxidase, as well as to design and test novel therapeutic agents for diseases associated with developing oxidative stress.

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The authors declare that there are no conflicts of interest present.