danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2020-64-2-193-198dan-870Research ArticleБИОЛОГИЯBIOLOGYМолекулярные механизмы высокоаффинного взаимодействия белка tBid с митохондриальным комплексом МТСН2-МОАР-1Molecular mechanisms of high-affinity interaction of the protein tBid with the mitochondrial complex МТСН2-МОАР-1ДудкоА. В.DudkoH. V.

Дудко Анна Викторовна – мл. науч. сотрудник.

ул. Академическая, 27, 220072, Минск

Dudko Hanna V. – Junior researcher.

27, Akademicheskaya Str., 220072, Minsk

dudko@ibp.org.byУрбанВ. А.UrbanV. A.

Урбан Виктор Андреевич – мл. науч. сотрудник.

ул. Академическая, 27, 220072, Минск

Urban Viktar A. – Junior researcher.

27, Akademicheskaya Str., 220072, Minsk

urban@ibp.org.byВересовВ. Г.VeresovV. G.

Вересов Валерий Гавриилович – д-р биол. наук, гл. науч. сотрудник.

ул. Академическая, 27, 220072, Минск

Veresov Valery G. – D. Sc. (Biology), Chief researcher.

27, Akademicheskaya Str., 220072, Minsk

veresov@ibp.org.byИнститут биофизики и клеточной инженерии Национальной академии наук БеларусиInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus202017052020642193198Copyright © Дудко А.В., Урбан В.А., Вересов В.Г., 20202020Дудко А.В., Урбан В.А., Вересов В.Г.Dudko H.V., Urban V.A., Veresov V.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/870

Митохондриальный белок MTCH2 (mitochondrial carrier homolog 2) играет важную роль в осуществлении апоптоза, являясь рецептором для про-апоптотического белка tBid на наружной мембране митохондрий (НММ). Ранее было установлено, что белок MOAP-1 (modulator of apoptosis-1) необходим для эффективного связывания белка tBid с его рецептором на НММ, однако структурные детерминанты этой регуляции остаются неясными. В данном сообщении представлены результаты установления структурных факторов действия белка MOAP-1, обусловливающих эффективное связывание белка tBid с рецептор-подобным белком MTCH2

Mitochondrial carrier homolog 2 (MTCH2) is a protein that plays an important role in the execution of apoptosis being a receptor for tBid in the outer membrane of mitochondria. Previously, it has been shown that the binding of the modulator of apoptosis-1 (MOAP-1) protein to MTCH2 is required for the efficient MTCH2-mediated recruitment of tBid to mitochondria and, in contrast, tBid is required for the MOAP-1 recruitment to mitochondria, but the structure understanding of these phenomena is absent. In this study, we have provided structural insights into the mechanisms of regulation of the MTCH2 receptor function for tBid by MOAP-1.

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The authors declare that there are no conflicts of interest present.