danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2020-64-3-317-324dan-886Research ArticleБИОЛОГИЯBIOLOGYМолекулярно-генетические механизмы регуляции дигидрофлавонол редуктазы и транскрипционного фактора Hy5 экзогенной 5-аминолевулиновой кислотой в проростках озимого рапсаMolecular-genetic mechanisms of regulation of dihydroflavonol reductase and transcription factor Hy5 by exogenous 5-aminolevulinic acid in winter rape plantsАверинаН. Г.AverinaN. G.

Аверина Наталия Георгиевна – д-р биол. наук, профессор, гл. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Averina Natalia G. – D. Sc. (Biology), Professor, Chief researcher

27, Akademicheskaya Str., 220072, Minsk

averina_ng@tyt.byЕмельяноваА. В.YemelyanavaH. V.

Емельянова Анна Викторовна – мл. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Yemelyanava Hanna V. – Junior researcher

27, Akademicheskaya Str., 220072, Minsk

yashchuk.anna@mail.ruКалягаТ. Г.KaliahaT. G.

Каляга Татьяна Геннадьевна – мл. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Kaliaha Tatsiana G. – Junior researcher

27, Akademicheskaya Str., 220072, Minsk

t_kalyaga@mail.ruСавинаС. М.SavinaS. M.

Савина Светлана Михайловна – канд. биол. наук, cт. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Savina Sviatlana M. – Ph. D. (Biology), Senior researcher

27, Akademicheskaya Str., 220072, Minsk

svetlanapavluchkova@yandex.ruИнститут биофизики и клеточной инженерии Национальной академии наук БеларусиInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus202009072020643317324Copyright © Аверина Н.Г., Емельянова А.В., Каляга Т.Г., Савина С.М., 20202020Аверина Н.Г., Емельянова А.В., Каляга Т.Г., Савина С.М.Averina N.G., Yemelyanava H.V., Kaliaha T.G., Savina S.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/886

Изучено влияние экзогенной 5-аминолевулиновой кислоты (АЛК) на активность дигидрофлавонол-4-редуктазы (DFR), экспрессию dfr гена и hy5 гена транскрипционного фактора Hy5, а также действие света разной интенсивности в сочетании с действием АЛК на накопление антоцианов в семядольных листьях озимого рапса (Brassica napus L.). Показано, что стимуляция накопления антоцианов под действием экзогенной АЛК на молекулярном уровне обеспечивается повышением уровня экспрессии dfr и hy5 генов, а также возрастанием активности DFR фермента. Увеличение интенсивности света от 40,5 до 66,2 мкмоль фотонов/м2·с приводило к повышению способности растений накапливать антоцианы в среднем на 35 %. Действие АЛК в концентрациях 50, 100, 150 и 200 мг/л приводило к дополнительному усилению накопления антоцианов при двух используемых уровнях освещенности, причем в дозозависимой манере. При этом величина стимулирующего эффекта АЛК при использовании света высокой интенсивности была выше, чем в случае более низкой освещенности. Так, стимуляция накопления антоцианов при освещенности 40,5 мкмоль фотонов/м2·с составила 106 % при использовании 50 мг/л АЛК, 165 % – при использовании 100 мг/л АЛК, 222 % – в случае 150 мг/л АЛК и 350 % – при действии 200 мг/л АЛК по сравнению со световым контролем без обработки растений АЛК. При освещенности 66,2 мкмоль фотонов/м2·с эти показатели были 164, 262, 359 и 583 % соответственно по отношению к световому контролю. Таким образом, продемонстрировано, что стимуляция накопления антоцианов под действием АЛК в растениях озимого рапса обусловлена на молекулярном уровне ее влиянием на транскрипцию dfr и hy5 генов.

The effect of exogenous 5-aminolevulinic acid (ALA) on the activity of dihydroflavonol-4-reductase (DFR), the expression of the dfr gene and the hy5 gene of the transcription factor Hy5 and the light effect of different intensities in combination with the ALA action on the accumulation of anthocyanins in cotyledonous leaves of winter rape (Brassica napus L.) were studied. It was shown that the stimulation of the accumulation of anthocyanins under the exogenous ALA action at the molecular level was provided by increasing the expression level of the dfr and hy5 genes and the activity of the DFR enzyme. Increasing the light intensity from 40.5 to 66.2 μmol photons/m2·s enhanced the ability of plants to accumulate anthocyanins on average by 35 %. The ALA action at concentrations of 50, 100, 150 and 200 mg/L led to an additional increase in the accumulation of anthocyanins at the two used levels of illumination, and in a dose-dependent manner. The stimulating effect of ALA under high light intensity was much higher than in the case of lower illumination. Thus, the stimulation of the anthocyanin accumulation under illumination of 40.5 μmol photons/m2·s was 106 % when using 50 mg/L ALA, 165 % – when using 100 mg/L ALA, 222 % – in the case of 150 mg/L ALA and 350 % – under the action of 200 mg/L ALA compared with light control without of ALA treatment. At an illumination of 66.2 μmol photons/m2·s, these indicators were 164, 262, 359 and 583 % respectively. Thus, it was demonstrated that the stimulation of the accumulation of anthocyanins under the action of ALA in winter rape plants was due to its positive effect on the transcription of the dfr and hy5 genes at the molecular level.

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The authors declare that there are no conflicts of interest present.