danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2021-65-4-448-455dan-992Research ArticleБИОЛОГИЯBIOLOGYПолучение цельноорганного скаффолда печени крысыEngineering a whole rat liver scaffoldДубкоА. Д.DubkoA. D.

Дубко Андрей Дмитриевич – мл. науч. сотрудник

ул. Долгобродская, 23/1, 220070, Минск

Dubko Andrei D. – Junior researcher

23/1, Dolgobrodskaya Str., 220070, Minsk 

dubko.immun@gmail.comЮркевичМ. Ю.YurkevichM. Yu.

Юркевич Мария Юрьевна – канд. биол. наук, доцент

ул. Долгобродская, 23/1, 220070, Минск

Yurkevich Mariya Yu. – Ph. D. (Biology), Associate professor

23/1, Dolgobrodskaya Str., 220070, Minsk 

marija4567@gmail.comЛобайМ. В.LabaiM. V.

Лобай Марина Валерьевна – ст. преподаватель

ул. Долгобродская, 23/1, 220070, Минск

Labai Marina V. – Senior lecture

23/1, Dolgobrodskaya Str., 220070, Minsk 

marina.lobai@mail.ruСвирскаяА. В.SvirskayaA. V.

Свирская Алеся Валерьевна – студент

ул. Долгобродская, 23/1, 220070, Минск

Svirskaya Alesia V. – Student

23/1, Dolgobrodskaya Str., 220070, Minsk 

alesjswirskay@mail.ruЮхановН. Г.YukhanovN. G.

Юханов Никита Геннадьевич – врач-патологоанатом

ул. Фрунзенская, д. 43, д. Боровляны, Минский р-н

Yukhanov Nikita G. – Pathologist

43, Frunzenskaya Str., Borovlyany vill., Minsk district 

YkhanovN@yandex.byСавицкаяТ. В.SavitskayaT. V.

Савицкая Татьяна Владимировна – канд. биол. наук, врач

ул. Фрунзенская, д. 43, д. Боровляны, Минский р-н

Savitskaya Tatiana V. – Ph. D. (Biology), Doctor

43, Frunzenskaya Str., Borovlyany vill., Minsk district 

tat_savitskaya@mail.comНижегородоваД. Б.NizheharodavaD. B.

Нижегородова Дарья Борисовна – канд. биол. наук, доцент

ул. Долгобродская, 23/1, 220070, Минск

Nizheharodava Daria B. – Ph. D. (Biology), Associate professor

23/1, Dolgobrodskaya Str., 220070, Minsk 

nzh@tut.byЗафранскаяМ. М.ZafranskayaM. M.

Зафранская Марина Михайловна – д-р мед. наук, доцент, заведующий кафедрой

ул. Долгобродская, 23/1, 220070, Минск

Zafranskaya Marina M. – D. Sc. (Medicine), Head of the Department

23/1, Dolgobrodskaya Str., 220070, Minsk 

zafranskaya@gmail.comМеждународный государственный экологический институт им. А. Д. Сахарова Белорусского государственного университетаInternational Sakharov Environmental Institute of the Belarusian State UniversityРеспубликанский научно-практический центр детской онкологии, гематологии и иммунологииBelarusian Research Center for Pediatric Oncology, Hematology and Immunology202102092021654448455Copyright © Дубко А.Д., Юркевич М.Ю., Лобай М.В., Свирская А.В., Юханов Н.Г., Савицкая Т.В., Нижегородова Д.Б., Зафранская М.М., 20212021Дубко А.Д., Юркевич М.Ю., Лобай М.В., Свирская А.В., Юханов Н.Г., Савицкая Т.В., Нижегородова Д.Б., Зафранская М.М.Dubko A.D., Yurkevich M.Y., Labai M.V., Svirskaya A.V., Yukhanov N.G., Savitskaya T.V., Nizheharodava D.B., Zafranskaya M.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/992

Получение трехмерного скаффолда открывает широкие перспективы для инженерии биоискусственных органов. В работе представлен метод перфузионной децеллюляризации печени крысы с анализом основных проблем и вариантов их решения. Перфузия донорской печени 0,1 %-ным раствором додецилсульфата натрия (SDS) позволяет получить качественный бесклеточный матрикс, характеризующийся сохранностью печеночной архитектоники, проходимостью сосудистого русла, остаточной ДНК менее 1 %, отсутствием признаков деструкции коллагеновых волокон и тканевого отека. Полученный предложенным методом скаффолд может использоваться для рецеллюляризации аллогенными клеточными культурами при создании объемных тканеинженерных конструкций.

Engineering a three-dimensional scaffold opens up great prospects for creation of manufacturing biological artificial organs. The article presents a method of perfusion decellularization of a rat liver, with the main problems and options for their solution being analyzed. Perfusion of a donor liver with 0.1 % a sodium dodecyl sulfate (SDS) solution allows obtaining a high-quality cell-free matrix characterized by preserved hepatic architectonics, patent vascular bed, residual DNA of less than 1 %, no signs of collagen fibers destruction and tissue edema. The obtained scaffold can be used for recellularization by allogeneic cell cultures when creating volumetric tissue-engineered designs.

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The authors declare that there are no conflicts of interest present.