danДоклады Национальной академии наук БеларусиDoklady of the National Academy of Sciences of Belarus1561-83232524-2431The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1561-8323-2021-65-4-456-465dan-993Research ArticleБИОЛОГИЯBIOLOGYЗависимость экспрессии генов кальциевой сигнальной системы в клетках верхушечных листьев растений томата от гравистимуляции и обработки этефономDependence of expression of genes controlling calcium signaling on gravistimulation in cells of tomato apical leaves and treatment by etephonСуховееваС. В.SukhaveyevaS. V.

Суховеева Светлана Владимировна – научный сотрудник

ул. Академическая, 27, 220072, Минск

Sukhaveyeva Sviatlana V. – Researcher

27, Akademicheskaya Str., 220072, Minsk 

suhoveevalmbc@mail.ruКабачевскаяЕ. М.KаbachevskayaA. М.

Кабачевская Елена Михайловна – канд. биол. наук, вед. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Kabachevskaya Alena M. – Ph. D. (Biology), Leading researcher

27, Akademicheskaya Str., 220072, Minsk 

lmbc@ibp.ogr.byВолотовскийИ. Д.VolotovskiI. D.

Волотовский Игорь Дмитриевич – академик, д-р биол. наук, профессор, гл. науч. сотрудник

ул. Академическая, 27, 220072, Минск

Volotovski Igor D. – Academician, D. Sc. (Biology), Professor, Chief researcher

27, Akademicheskaya Str., 220072, Minsk 

volotovski@yahoo.comИнститут биофизики и клеточной инженерии Национальной академии наук БеларусиInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus202102092021654456465Copyright © Суховеева С.В., Кабачевская Е.М., Волотовский И.Д., 20212021Суховеева С.В., Кабачевская Е.М., Волотовский И.Д.Sukhaveyeva S.V., Kаbachevskaya A.М., Volotovski I.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://doklady.belnauka.by/jour/article/view/993

С использованием ОТ-ПЦР в режиме реального времени выявлена чувствительность к гравистимуляции экспрессии на уровне транскрипции трех генов, кодирующих белки, участвующие в сигнальной кальциевой трансдукции. На ранних этапах ответа, начиная с 15–60 мин действия гравистимула для трех генов (SCA2, РВР2, САМ2) показано увеличение образования транскриптов. Обработка растений перед началом гравистимуляции этефоном (источником экзогенного этилена) приводила к изменению характера модуляции экспрессии изученных генов в ответ на гравистимул. Рассматривается роль кальциевого метаболизма в клетке в реализации финальных стадий гравитропического ответа растения.

The sensitivity of expression at the level of transcription of genes encoding proteins involved in calcium signal transduction to gravistimulation was revealed using real-time RT-PCR. For three genes SCA2, РВР2, САМ2, the increase in the transcript formation was shown at early response stages, starting from 15–60 minute gravistimulus. The treatment of plants before the start of gravistimulation with an ethephon (source of exogenous ethylene) led to a change in the modulation of expression of the studied genes in response to gravistimulus. The role of calcium metabolism in realization of final steps of gravitropism reaction is considered.

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The authors declare that there are no conflicts of interest present.