Platelet gel and factors determining its biological activity
https://doi.org/10.29235/15618323-2023-67-6-490-498
Abstract
A platelet gel (PG) derived from platelet concentrate (PC) is considered as a perspective therapeutic agent with hemostatic and regenerative properties. PG was obtained from PC separated from human peripheral blood by automatic apheresis by adding human thrombin (30 U/ml). We compared the proliferation of human mesenchymal stromal cells (MSCs) in vitro in the presence of PG and the dependence of gel density on excess of fibrinogen, the presence of calcium chloride, calcium gluconate, and aprotinin. PG was formed from CT in the presence of thrombin during 5–10 minutes. PG as gel-like fibrin membrane contained platelets and an admixture of leukocytes, and was capable to enhance the proliferation of MSCs in vitro. The presence of calcium gluconate (10 mg/ml) increased in the presence of PG the rate of MSCs proliferation in vitro. The presence of aprotinin in PG at a concentration of 10–1000 KIU/ml caused a dose-dependent decrease in the rate of gel biodegradation and did not affect the ability of PG to stimulate the proliferation of human MSCs in vitro.
Supporting agencies: The Ministry of Health of the Republic of Belarus (grant no. 20221498) supported the work
About the Authors
V. Yu. Galitskaya
Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies
Belarus
Belarus
Galitskaya Viktoriya Yu. – Junior Researcher.
Dolginovsky tract, 160, 220080, Minsk
M. P. Potapnev
Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies
Belarus
Belarus
Potapnev Michael P. – D. Sc. (Medicine), Professor, Head of the Department.
Dolginovsky tract, 160, 220080, Minsk
V. I. Asaevich
Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies
Belarus
Belarus
Asaevich Vadim I. – Junior Researcher.
Dolginovsky tract, 160, 220080, Minsk
V. G. Bogdan
Department of the Medical Sciences of the National Academy of Sciences of Belarus
Belarus
Belarus
Bogdan Vasiliy G. – D. Sc. (Medicine), Professor, Academician-Secretary.
66, Nezavisimosti Ave., 220072, Minsk
S. M. Kosmacheva
Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies
Belarus
Belarus
Kosmacheva Svetlana M. – Ph. D. (Medicine), Head of the Laboratory.
Dolginovsky tract, 160, 220080, Minsk
F. N. Karpenko
Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies
Belarus
Belarus
Karpenko Fedor N. – Ph. D. (Medicine), Director.
Dolginovsky tract, 160, 220080, Minsk
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