Phosphatidacylhydrolase as a virulence factor of opportunistic microorganisms in lipoconjugate hydrolysis reactions
https://doi.org/10.29235/1561-8323-2026-70-1-32-44
Abstract
For the first time, phosphatidyl derivatives of a number of nucleosides were studied as substrates of phosphatidylacylhydrolase in phospholipolysis reactions. A new trigger mechanism has been proposed and studied that initiates the death of pathogenic and opportunistic microorganisms (Staphylococcus aureus and Candida spp. cells) due to their virulence, including the activity of their own type A phospholipases, under the action of medicinal forms of antibiotics (based on covalently and non-covalently modified lipoconjugates) of the purine (nelarabine, nitrogenous base guanine) and pyrimidine (brivudine, nitrogenous base uracil) series, which have a bactericidal effect. In terms of the resistance of forms to destruction in the digestive tract, it has been shown that brivudine lipoconjugates are most susceptible to hydrolysis with the participation of pancreatic phospholipase A2, while nelarabine-based lipoconjugate is characterized by higher stability to phospholipolysis. It has been established that, since the rate of hydrolysis of lipoconjugates of nelarabine and brivudine by phospholipase A of the culture fluid of Candida sake and Staphylococcus aureus is 1.7 and 2 times higher, respectively, than that of phosphatidylcholine, they can serve as a basis for the demonstration of biocide-containing conjugates and facilitate the availability of the active substance due to increased hydrolyzability.
About the Authors
N. M. Litvinko
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Litvinko Natalia M. – D. Sc. (Chemistry), Professor, Head of the Laboratory.
5/2, Kuprevich Str., 220084, Minsk
D. O. Gerlovsky
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Gerlovsky Denis O. – Ph. D. (Chemistry), Assistant Professor, Researcher.
5/2, Kuprevich Str., 220084, Minsk
I. A. Mikhailopulo
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Russian Federation
Russian Federation
Mikhailopulo Igor A. – Corresponding Member, D. Sc. (Chemistry), Chief Researcher.
5/2, Kuprevich Str., 220084, Minsk
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