Analysis of antimicrobial metabolites of bacteria Bacillus amyloliquefaciens BIM B-1125 – the basis of Baсto-health biological preparation
https://doi.org/10.29235/1561-8323-2022-66-3-321-329
Abstract
Antimicrobial metabolites of bacterial strain B. amyloliquefaciens BIM B-1125, the basis of Bacto-health preparation, demonstrating antagonism against the representatives of the opportunistic microbiota of valuable fish species, were isolated and characterized. Extracellular localization of antimicrobial compounds was established; their stability is shown in the temperature range of 50–100 °С and in the pH range of 2–10. The lipopeptide nature of antimicrobial metabolites was shown using thin layer chromatography. The mass spectrometric analysis of the active fraction confirmed the production of lipopeptide metabolites belonging to the iturin and surfactin families (iturin A, iturin A4, iturin A6-A7 isomers, surfactin A, surfactin C, and surfactin B isomers).
About the Authors
K. V. Kantor
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Kantor Karina V. – Researcher
2, Kuprevich Str., 220141, Minsk
I. A. Proskurnina
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Proskurnina Irina A. – Head of the Sector
2, Kuprevich Str., 220141, Minsk
N. V. Sverchkova
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Sverchkova Natalia V. – Ph. D. (Biology), Leading Researcher.
2, Kuprevich Str., 220141, Minsk
E. I. Kolomiets
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Kolomiets Emilia I. – Academician, D. Sc. (Biology), Professor, Chief Researcher
2, Kuprevich Str., 220141, Minsk
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