KASP genotyping of loci associated with the “1000-grain weight” trait in common wheat (Tríticum aestívum L.)
https://doi.org/10.29235/1561-8323-2023-67-3-214-221
Abstract
Using the KASP technology, the allelic composition of TaTGW6-A1, TaGASR7-A1, TaCKX6-D1, and TaGs3-D1 loci associated with the 1000-grain weight trait was identified in 25 varieties and variety accessions of the common wheat of Belarusian and foreign breeding. Annotated DNA sequences were selected for modeling and synthesis of KASP primers. It was demonstrated that the examined genotypes had carried both favorable alleles associated with 1000-grain weight, and the alleles producing a negative effect on the trait under study. Six wheat accessions of Belarusian breeding were identified. They carried a complex of alleles positively correlating with the 1000-grain weight trait: variety Vestochka-17, accessions E-2318, E-2263, E-2298, E-1569, and E-2695.
Keywords
About the Authors
V. A. Lemesh
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Lemesh Valiantsina A. – Ph. D. (Biology), Assistant Professor, Head of the Laboratory
27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus
S. I. Gryb
Research and Practical Center of the National Academy of Sciences of Belarus for Arable Farming
Belarus
Belarus
Stanislau Gryb – Academician, D. Sc. (Agrarian), Professor, Chief Researcher
1, Timiryazev Str., 222160, Zhodino, Minsk Region, Republic of Belarus
A. V. Lagunovskaya
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Lahunovskaya Alena V. – Senior Researcher
27, Akademicheskaya Str., 220072, Minsk, Republic
of Belarus
V. N. Kipen
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Kipen Viachaslau N. – Ph. D. (Biology), Leading Researcher
27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus
A. A. Buloichik
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Buloichik Andrei A. – Ph. D. (Biology), Leading Researcher
27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus
V. N. Bushtevich
Research and Practical Center of the National Academy of Sciences of Belarus for Arable Farming
Belarus
Belarus
Bushtevich Victar N. – Ph. D. (Agrarian), Assistant Professor, Head of the Laboratory
1, Timiryazev Str., 222160, Zhodino, Minsk Region, Republic of Belarus
V. I. Sakovich
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Sakovich Valiantsina I. – Researcher
27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus
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