Genetic diversity of Belarusian populations of Liparis loeselii (L.) Rich. (Orchidaceae)
https://doi.org/10.29235/1561-8323-2025-69-3-214-221
Abstract
The article presents the results of a study of the genetic diversity of Liparis loeselii (L.) Rich. populations using molecular iPBS markers. Samples for analysis were selected from six populations located at the southern border of the species’ range in the Grodno, Minsk, and Vitebsk regions of Belarus. The findings indicate that higher levels of genetic diversity are associated with populations that exhibit larger areas, greater numbers of plants, and potentially older ages of the population itself. The predominance of intrapopulation genetic diversity (74 %), as well as a low level of gene flow, indicate that genetic drift significantly affects the formation of the genetic structure of L. loeselii. The obtained data generally indicate a low adaptive potential of L. loeselii. Сonsequently, an unfavorable forecast for its conservation in the wild is provided, taking into account the current climate dynamics, as well as successional changes occurring within the bog biotopes.
About the Authors
N. V. HudnayaBelarus
Hudnaya Natalia V. – Reseаrcher
2v, Surganov Str., 220012, Minsk
A. N. Mialik
Belarus
Mialik Aliaksandr N.– Ph. D. (Biology), Head of the Sector
2v, Surganov Str., 220012, Minsk
V. V. Titok
Belarus
Titok Vladimir V. – Corresponding Member, D. Sc. (Biology), Assistant Professor, Chief Researcher
2v, Surganov Str., 220012, Minsk
References
1. Kachanovskii I. M., Nikiforov M. E., Parfenov V. I., Borodin O. I., Pugachevskii A. V., Baichorov V. M., Gapienko O. S., Giryaev A. S., Evdaseva T. P. Red Book of the Republic of Belarus. Plants: rare and endangered species of wild plants. 4th ed. Minsk, 2015. 445 p. (in Russian).
2. Senator S. A., Tretyakova A. S., Grudanov N. Yu., Shilov D. S., Philippov D. A. Liparis loeselii (Orchidaceae) in the Sverdlovsk region, Russia. Botanicheskii zhurnal = Botanical Journal, 2022, vol. 107, no. 3, pp. 302–306 (in Russian).
3. Khomutovskiy M. I. Biology and ecology of Liparis loeselii (L.) Rich. (Orchidaceae Juss.) in the Tver region. Izvestiya Samarskogo nauchnogo tsentra RAN = News of the Samara Scientific Center of the RAS, 2013, vol. 15, no. 3–7, pp. 2105–2115 (in Russian).
4. Rolfsmeier S. B. Liparis loeselii (L.) Rich. (yellow widelip orchid): a technical conservation assessment. Prepared for the USDA Forest Service, Rocky Mountain Region, Species Conservation Project. Available at: https://www.fs.usda.gov/Internet/FSE_DOCUMENTS/stelprdb5206990.pdf (accessed: 03.10.2024).
5. McMaster R. T. The population biology of Liparis loeselii, Loesel’s twayblade, in a Massachusetts wetland. Northeastern Naturalist, 2001, vol. 8, no. 2, pp. 163–178. https://doi.org/10.2307/3858369
6. Milanovic D. Liparis loeselii (L.) Rich. – a plant rediscovered in the Balkan peninsula. Botanica Serbica, 2012, vol. 36, no. 2, pp. 85–89.
7. Lebedko V. N. A rare representative of the Orchidaceae family Juss. Liparis loeselii (L.) Rich. in the Gomel region of Belarus. Okhrana i kul’tivirovanie orkhidei: materialy X Mezhdunarodnoi nauchnoprakticheskoi konferentsii [Protection and cultivation of orchids: Proceedings of the X International scientific and practical conference]. Minsk, 2015, pp. 130–133 (in Russian).
8. Leimu R., Mutikainen P., Koricheva J., Fischer M. How general are positive relationships between plant population size, fitness and genetic variation? Journal of Ecology, 2006, vol. 94, no. 5, pp. 942–952. https://doi.org/10.1111/j.13652745.2006.01150.x
9. Samantaray S., Ngangkham U. Evaluation of Genetic Diversity in Chlorophytum borivilianum (Santp. and Fernan.) Using Molecular Markers: An Endangered Medicinal Plant. Active Ingredients from Aromatic and Medicinal Plants, 2017. https://doi.org/10.5772/66536
10. Efimov P. G. Orchids of the northwest of European Russia (Leningrad, Pskov, Novgorod regions). 2nd ed. Moscow, 2012. 220 p. (in Russian).
11. Khlyzova N. Yu., Skolzneva L. N., Nedosekina T. V. Rare species Cypripedium calceolus and Liparis loeselii (Orchidaceae) also in the Lipetsk region. Botanicheskii zhurnal = Botanical Journal, 2009, vol. 94, no. 10, pp. 1594–1597 (in Russian).
12. Mialik A. N., Rovenskaya I. A., Fedyushko I. A., Ring V. I. Flora and vegetation of the local reserve “Urochische Krasnoe” (Ivyevsky district, Grodno region). Aktual’nye problemy izucheniya i sokhraneniya fito- i mikobioty: materialy III Mezhdunarodnoi nauchnoprakticheskoi konferencii [Actual problems of studying and preserving phyto- and mycobiota: Proceedings of the III International scientific and practical conference]. Minsk, 2020, pp. 49–54 (in Russian).
13. Kalendar R., Antonius K., Smýkal P., Schulman A. H. iPBS: a universal method for DNA fingerprinting and retrotransposon isolation. Theoretical and Applied Genetics, 2010, vol. 121, pp. 1419–1430. https://doi.org/10.1007/s00122-0101398-2
14. Belogrudova I., Grauda D., Jakobsone G., Roze D., Ornicans R., Fokina O., Rashal I. Usability of retrotransposone-based molecular marker system to assess genetic diversity of Liparis loeselii. Acta Biologica Universitatis Daugavpiliensis, 2012, vol. 12, no. 1, pp. 40–43.
15. Belogrudova I., Grauda D., Lapina L., Jakobsone G., Roze D., Ornicâns R., Fokina O., Rasha I. Genetic diversity of Liparis loeselii in Latvia. Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences, 2018, vol. 72, no. 6, pp. 341–348. https://doi.org/10.2478/prolas-2018-0042
16. Samokhvalova N. V., Mialik A. N., Shlapakova T. G., Kruchonok A. V. Evaluation of genetic variability of Goodyera repens (L.) R. Br. populations in Belarus using molecular iPBS markers. Botanika. Issledovaniya = Botany. Research, 2023, no. 52, pp. 28–37 (in Russian).
17. Hudnaya N. V., Mialik A. N., Kulahina T. G., Titok V. V. Genetic variability of Cephalanthera longifolia (L.) Fritsch populations in Belarus. Botanika. Issledovaniya = Botany. Research, 2024, no. 53, pp. 114–122 (in Russian).