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Биосинтез каротиноидов красным дрожжевым грибом Rhodotorula glutinis БИМ Y-159

https://doi.org/10.29235/1561-8323-2026-70-1-54-62

Abstract

Carotenogenesis, composition and ratio of individual carotenoid components synthesized by Rhodotorula glutinis BIM Y-159 was investigated. It was found that peak level of carotenoid production (5.7 mg/g biomass) was reached during submerged fermentation of yeast in media with high concentration of carbohydrates (glucose 11 %) and deficiency of nitrogen nutrition. Cu2+ ions in concentration 0.5 mM stimulated production of carotenoids in R. glutinis BIM Y-159 by 17.5–17.8 %. Under optimal conditions the produced carotenoid complex is represented by torulene, torulorhodin, ζand β-carotenes plus an unidentified component. β-Carotene, torulene, and torulorhodin amount for 16 %, 40 % and 32 % of carotenoids synthesized by R. glutinis BIM Y-159.

About the Authors

E. A. Shlakhotko
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus

2, Kuprevich Str., 220084, Minsk



L. I. Sapunova
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus

2, Kuprevich Str., 220084, Minsk



A. G. Lobanok
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus

2, Kuprevich Str., 220084, Minsk



P. S. Shabunya
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus

5/2, Kuprevich Str., 220084, Minsk



References

1. Ochoa-Viñals N., Alonso-Estrada D., Pacios-Michelena S., García-Cruz A., Ramos-González R., Faife-Pérez E., Michelena-Álvarez L. G., Martínez-Hernández J. L., Iliná A. Current advances in carotenoid production by Rhodotorula sp. Fermentation, 2024, vol. 10, no. 4, art. 190. https://doi.org/10.3390/fermentation10040190

2. Zoz L., Carvalho J. C., Soccol V. T., Casagrande T. C., Cardoso L. Torularhodin and torulene: bioproduction, properties and prospective applications in food and cosmetics – a review. Brazilian Archives of Biology and Technology, 2015, vol. 58, no. 2, pp. 278–288. https://doi.org/10.1590/S1516-8913201400152

3. Kot A. M., Błażejak S., Gientka I., Kieliszek M., Bryś J. Torulene and torularhodin: “new” fungal carotenoids for industry? Microbial Cell Factories, 2018, vol. 17, art. 49. https://doi.org/10.1186/s12934-018-0893-z

4. Kot A. M., Błażejak S., Kurcz A., Gientka I., Kieliszek M. Rhodotorula glutinis – potential source of lipids, carotenoids, and enzymes for use in industries. Applied Microbiology and Biotechnology, 2016, vol. 100, pp. 6103–6117. https://doi.org/10.1007/s00253-016-7611-8

5. Buzzini P., Martini A. Production of carotenoids by strains of Rhodotorula glutinis cultured in raw materials of agroindustrial origin. Bioresource Technology, 2000, vol. 71, no. 1, pp. 41–44. https://doi.org/10.1016/S0960-8524(99)00056-5

6. Marova I., Carnecka M., Halienova A., Certik M., Dvorakova T., Haronikova A. Use of several waste substrates for carotenoid-rich yeast biomass production. Journal of Environmental Management, 2012, vol. 95, pp. S338–S342. https://doi.org/10.1016/j.jenvman.2011.06.018

7. Cheng Y. T., Yang C. F. Using strain Rhodotorula mucilaginosa to produce carotenoids using food wastes. Journal of the Taiwan Institute of Chemical Engineers, 2016, vol. 61, pp. 270–275. https://doi.org/10.1016/j.jtice.2015.12.027

8. Sharma R., Ghoshal G. Optimization of carotenoids production by Rhodotorula mucilaginosa (MTCC-1403) using agro-industrial waste in bioreactor: A statistical approach. Biotechnology Reports, 2020, vol. 25, art. e00407. https://doi.org/10.1016/j.btre.2019.e00407

9. Alfaro J. M., Reyes S. I., Cristobal H. A., Martearena M. R., Baigorí M. D., Pera L. M. Remediation of sugarcane vinasse using Rhodotorula glutinis or Rhodotorula mucilaginosa: Biomass morphology and its potential technological applications. Biocatalysis and Agricultural Biotechnology, 2024, vol. 58, art. 103193. https://doi.org/10.1016/j.bcab.2024.103193

10. Kot A. M., Sęk W., Kieliszek M., Błażejak S., Pobiega K., Brzezińska R. Diversity of red yeasts in various regions and environments of Poland and biotechnological potential of the isolated strains. Applied Biochemistry and Biotechnology, 2024, vol. 196, pp. 3274–3316. https://doi.org/10.1007/s12010-023-04705-5

11. Weber R. W. S., Anke H., Davoli P. Simple method for the extraction and reversed-phase high-performance liquid chromatographic analysis of carotenoid pigments from red yeasts (Basidiomycota, Fungi). Journal of Chromatography A, 2007, vol. 1145, no. 1–2, pp. 118–122. https://doi.org/10.1016/j.chroma.2007.01.052

12. Sebastian S., Tripathi A. D., Paul V., Darani K. K., Agarwal A. Production kinetics and characterization of natural food color (torularhodin) with antimicrobial potential. Bioresource Technology Reports, 2023, vol. 24, art. 101652. https://doi.org/10.1016/j.biteb.2023.101652

13. Britton G. General carotenoid methods. Methods in Enzymology, 1985, vol. 111, pp. 113–149. https://doi.org/10.1016/s0076-6879(85)11007-4

14. Kot A. M., Błażejak S., Kieliszek M., Gientka I., Bryś J., Reczek L., Pobiega K. Effect of exogenous stress factors on the biosynthesis of carotenoids and lipids by Rhodotorula yeast strains in media containing agro-industrial waste. World Journal of Microbiology and Biotechnology, 2019, vol. 35, art. 157. https://doi:10.1007/s11274-019-2732-8

15. Sakaki H., Nakanishi T., Tada A., Miki W., Komemushi S. Activation of torularhodin production by Rhodotorula glutinis using weak white light irradiation. Journal of Bioscience and Bioengineering, 2001, vol. 92, pp. 294–297. https://doi.org/10.1016/S1389-1723(01)80265-6

16. Simpson K. L., Nakayama T. O., Chichester C. O. Biosynthesis of yeast carotenoids. Journal of Bacteriology, 1964, vol. 88, no. 6, pp. 1688–1694. https://doi.org/10.1128/jb.88.6.1688-1694.1964

17. Frengova G., Simova E., Pavlova K., Beshkova D., Grigorova D. Formation of carotenoids by Rhodotorula glutinis in whey ultrafiltrate. Biotechnology and Bioengineering, 1994, vol. 44, no. 8, pp. 888–894. https://doi.org/10.1002/bit.260440804


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ISSN 1561-8323 (Print)
ISSN 2524-2431 (Online)