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PRODUCTION OF RECOMBINANT HUMAN LACTOFERRIN FROM THE MILK OF GOAT-PRODUCERS AND ITS PHYSIOLOGICAL EFFECTS

Abstract

Primary male-goats Lac-1 (human lactoferrin gene construction hLF5) and Lac-2 (human lactoferrin gene construction hLF3) with a genome containing a human lactoferrin gene were bred and the sperm bank of primary male-goats was created within the framework of Belarus-Russia Union State programs. The herd of goats that produced recombinant human lactoferrin (rhLF) in their milk was obtained. Human lactoferrin (rhLF) from the milk of transgenic goats, natural human lactoferrin (nhLf) from the woman milk, and natural goat lactoferrin (gLf) from the milk of non-transgenic goats were purified using cation-exchange chromatography. Physicochemical characteristics of rhLF were similar to those of nhLf as revealed by different analytical methods including mass spectrometry and peptide mapping. g. Oral supplementation with rhLF activated lipid metabolism and steroidogenesis, promoted favorable changes in the gut microflor composition as well as the enhancement of the functional activity and the fine structure of the rat liver and gut.

About the Authors

V. S. LUKASHEVICH
Institute of Physiology of the National Academy of Sciences of Belarus, Minsk
Belarus


A. I. BUDZEVICH
Scientific and practical center of the National Academy of Sciences of Belarus on animal husbandry, Zhodino
Belarus


I. V. SEMAK
Belarusian State University, Minsk
Belarus


V. N. KUZNETSOVA
Scientific and practical center of the National Academy of Sciences of Belarus on animal husbandry, Zhodino
Belarus


E. V. MALYUSHKOVA
Belarusian State University, Minsk
Belarus


A. E. PYZH
Institute of Physiology of the National Academy of Sciences of Belarus, Minsk
Belarus


S. A. NOVAKOVSKAYA
Institute of Physiology of the National Academy of Sciences of Belarus, Minsk
Belarus


J. A. RUDNICHENKO
Institute of Physiology of the National Academy of Sciences of Belarus, Minsk
Belarus


N. A. POPKOV
Scientific and practical center of the National Academy of Sciences of Belarus on animal husbandry, Zhodino
Belarus


O. A. IVASHKEVICH
Belarusian State University, Minsk
Belarus


I. V. ZALUTSKY
Institute of Physiology of the National Academy of Sciences of Belarus, Minsk
Belarus


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ISSN 1561-8323 (Print)
ISSN 2524-2431 (Online)