NEW REAGENT FOR LABELING PROTEINS WITH RARE EARTH METAL IONS
Abstract
Activated ester of a carboxylic derivative of europium diethylenetriaminetetraacetate has been synthesized by the interaction of europium salt of diethylenetriaminepentaacetic acid aminoethylamide with di-N-succinimidyl-p-phthalate. This reagent was applied to introduce rare earth metal ions in animal immunoglobulins (monoclonal antibodies). The proteins labelled with Eu3+ provide the required characteristics of fluorescence intensity, background, sensitivity and selectivity in diagnostic systems of lanthanide immunofluorometric assay.
About the Authors
O. S. GARBUZ
Институт биоорганической химии НАН Беларуси, Минск
Belarus
Belarus
L. V. DUBOVSKAYA
Институт биоорганической химии НАН Беларуси, Минск
Belarus
Belarus
O. V. SVIRIDOV
Институт биоорганической химии НАН Беларуси, Минск
Belarus
Belarus
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