Preview

Doklady of the National Academy of Sciences of Belarus

Advanced search

COW OOCYTE PICK-UP BY THE TRANSVAGINAL PUNCTURE OF FOLLICLES

Abstract

The results of studying the influence of some factors on the efficiency of the cattle pick-up are presented in the article. It is established that the level of recoverability of the oocyte-cumulus complexes from the number of punctured follicles is 68.7%. The yield of oocytes per one aspiration is 2.7, including per one positive aspiration for extraction – 3.3. Most acceptable for aspiration is the 17G dia needle regardless of the vacuum level. The greatest number of aspirated follicles (6.2 and 8.0) and picked-up oocytes (4.0 to 5.5) is observed when for simulation the follicle-stimulating Pluset hormone is used at a dose of 750 I. E. with or without the use of the progestagen drug Crestar during the stimulation process.

About the Authors

V. K. PESTIS
Grodno State Agrarian University, Grodno
Belarus


L. V. GOLUBETS
Grodno State Agrarian University, Grodno
Belarus


A. S. DESHKO
Grodno State Agrarian University, Grodno
Belarus


I. S. KYSSA
Grodno State Agrarian University, Grodno
Belarus


M. V. POPOV
«Pochapovo», Pinsk
Belarus


References

1. Transvaginal ultrasound guided follicular aspiration of bovine oocytes / M. C. Pieterse [et al.] // Theriogenology. – 1991. – Vol. 35. – P. 19–24.

2. Effects of aspiration vacuum and needle diameter on cumulus oocyte complex morphology and developmental capacity of bovine oocytes / P. Bols [et al.] // Theriogenology. – 1996. – Vol. 45. – P. 1001–1014.

3. Bols, P. Gebruik van de transvaginale Ovum Pick-Up (OPU) techniek : geboorte van de eerste OPU kalveren in Belgie. (Use of transvaginal oocyte pick-up: first OPU calves bom in Belgium) / P. Bols, A. Soom Van, A. de Kruif // Vlaams Diergeneeskundig Tijdschrift. – 1996. – Vol. 65. – P. 86–91.

4. Potential use of Ovum Pick-Up for embryo production and breeding in cattle / T. Kruip [et al.] // Theriogenology. – 1994. – Vol. 42. – P. 675–683.

5. Boni, R. Impact of Ovum Pick-Up (OPU) technique for research and animal breeding / R. Boni, L. Zicarelli, T. Kruip // Reproduction and Animal Breeding: Advancesand Strategy. – Amsterdam, 1995. – P. 211–221.

6. Follicular dynamics, repeatability and predictability of follicular recruitment in cows submitted to repeated follicular puncture / R. Boni [et al.] // Theriogenology. – 1997. – Vol. 48. – P. 277–289.

7. Production, freezing and transfer of bovine IVF embryos and subsequent calving result / J. F. Hasler [et al.] // Theriogenology. – 1995. – Vol. 43. – P. 141–152.

8. Effect of follicle size on bovine oocyte quality and developmental competence folloving maturation and culture in vitro / P. Lonergan [et al.] // Mol. Reprod. Dev. – 1994. – Vol. 37. – P. 48–53.

9. Effect of the frequency of ovum pick-up intervals on follicle number, oocyte recovery and embryo production rates in cattle / K. Imai [et al.] // Theriogenology. – 2000. – Vol. 53. – P. 359.

10. Repeated transvaginal ultrasound-guided ovum pick-up in ECG-treated cows / M. C. Pieterse [et al.] // Theriogenology. – 1992. – Vol. 37. – P. 273.

11. Stubbings, R. B. Effect of ultrasonically-guided follicle aspiration on estrous cycle and follicular dynamics in Holstein cows / R. B. Stubbings, J. S. Walton // Theriogenology. – 1995. – Vol. 43. – P. 705–712.

12. Factors affecting recoverey and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology / F. A. Ward [et al.] // Theriogenology. – 2000. – Vol. 54. – P. 433–446.

13. De Ruigh, L. The effect of FSH stimulation prior to ovum pick-up on oocyte and embryo yield / L. De Ruigh, E. Mullaart, A. M. van Wagtendonk-de Leeuw // Theriogenology. – 2000. – Vol. 53. – P. 349.

14. Gonadotropin stimulation of cattle donors at estrus for transvaginal oocyte collection / J. B. Paul [et al.] // Theriogenology. – 1995. – Vol. 43. – P. 294.

15. Van Soom, A. Improved results in IVF-treatment of sterility patients by careful selection of bulls and by stimulation of cows with FSH before slaughter / A. Van Soom, P. E. J. Bols, A. de Kruif // Reprod. Dom. Anim. – 1995. – Suppl. 3. – P. 67.


Review

Views: 1068


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 1561-8323 (Print)
ISSN 2524-2431 (Online)