EVALUATION OF THE POLYMORPHISM ESR1-PVUII OF THE ESTROGEN RECEPTOR GENE IN PIGS USING THE PCR–RFLP METHOD AND THE HRM-ANALYSIS TECHNOLOGY
Abstract
The DNA typing for the estrogen receptor gene (ESR1) in pigs is proposed to carry out with the aid of the technology of the HRM-analysis (High Resolution Melting) and continue to use it as an express method for mass screening of breeding animals in order to increase the fertility and to improve the breeding animals process in the pig. Assessed polymorphism ESR1-PvuII of the estrogen receptor gene in the Belarusian large white pigs (n = 51) with the use of the HRM-analysis, which is highly sensitive and cost-effective as compared to the traditional PCR-RFLP method. The conditions for real-time PCR are worked out, the accuracy of identification of genotypes AA, BB, AB is confirmed, and the effectiveness of the HRM-method is also analyzed.
About the Authors
M. E. MIKHAILOVA
Институт генетики и цитологии НАН Беларуси, Минск
Belarus
Belarus
E. L. RAMANISHKA
Институт генетики и цитологии НАН Беларуси, Минск
Belarus
Belarus
References
1. Эрнст Л. К., Зиновьева Н. А. Биотехнология в животноводстве. М., 2008. – 510 с.
2. Terman A., Kmieć M., Polasik D. // Arch. Tierz., Dummerstorf. 2006. N 49. P. 71–76.
3. Сметник А. А. // Проблемы репродукции. 2011. № 3. С. 31–37.
4. Munoz G., Ovilo C., Estelle J. // Genet. Sel. 2007. Vol. 39. P. 195–206.
5. Зиновьева Н. А., Гладырь Е. А., Эрнст Л. К., Брем Г. Введение в молекулярно-генную диагностику сельскохозяйственных животных. М., 2002. С. 68–70.
6. Шейко И. П., Лобан Н. А., Василюк О. Я., Драбинович Д. С. // Весцi НАН Беларусi. Сер. аграрных навук. 2006. № 3. С. 77–81.
7. Short T. H., Rothschild M. F., Southwood O. I., McLaren D. G. // J. Animal Science. 1997. N 75. P. 3138–3142.
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