BINDING OF MAGNETIC GEL MICROSPHERES TO BLOOD MONONUCLEAR CELLS
Abstract
A polyvinyl alcohol (PVA) coating decreases the non-specific binding of magnetic microspheres (MMS) composed of glutaraldehyde-cross-linked chitosan to blood mononuclear cells (BMC) more efficiently than a carboxymethyldextran (carboxylation degree <10 %) coating. PVA-composed MMS coated with an additional PVA layer after glutaraldehyde cross-linking, including ones functionalized with carboxyl groups due to the H2O2 treatment, demonstrate low non-specific binding to BMC. In the pellets resulting from magnetic separation, there were 4670 ± 760 PVA MMS and 2040 ± 400 MagnosphereTM MS300/Carboxyl commercial MMS per one BMC.
About the Authors
K. V. LAZNEV
Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus, Minsk
Belarus
Belarus
V. E. AGABEKOV
Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus, Minsk
Belarus
Belarus
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