Preview

Doklady of the National Academy of Sciences of Belarus

Advanced search

TAUMATHIN-LIKE PROTEIN AND OXALATE OXIDASE AS MARKERS OF WINTER WHEAT (TRITICUM AESTIVUM L.) RESISTANCE TO POWDERY MILDEW (ERYSIPHE GRAMINIS)

Abstract

In the present article, we studied a correlation between an increased resistance of winter wheat seedlings of different varieties to powdery mildew and high levels of constitutive expression of genes encoding one of two pathogenesisrelated proteins: thaumatin-like protein (Tlp) or oxalate oxidase (OxOxid). Highly resistant varieties have shown either high Tlp expression and low OxOxid expression or high OxOxid expression and low Tlp expression. We propose to use a combination of both Tlp and OxOxid gene expression levels as a marker for resistance of winter wheat breeding material to powdery mildew. 

About the Authors

Mechislav S. Radyuk
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Minsk
Belarus

Ph. D. (Biology), Senior researcher

27, Akademicheskaya Str., 220072



Irina A. Dremuk
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Minsk
Belarus

Ph. D. (Biology), Researcher

27, Akademicheskaya Str., 220072



Yauhen V. Viasau
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Minsk
Belarus

Junior researcher

27, Akademicheskaya Str., 220072



Nikolai V. Shalygo
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Minsk
Belarus

Corresponding Member, D. Sc. (Biology), Head of the Laboratory

27, Akademicheskaya Str., 220072



References

1. Terras F. R. G., Eggermont K., Kovaleva V., Raikhel N. V., Osborn R. W., Kester A., Rees S. B., Torrekens S., Van Leuven F., Vanderleyden J., Cammue B. P. A., Broekaert W. F. Small cystein-rich antifungal proteins from radish: their role in host defense. The Plant Cell, 1995, vol. 7, no. 5, pp. 573–588. doi.org/10.2307/3870116

2. van Loon L. C., Rep M., Pieterse C. M. J. Significance of inducible defense-related proteins in infected plants. Annual Review of Phytopathology, 2006, vol. 44, no. 1, pp. 135–162. doi.org/10.1146/annurev.phyto.44.070505.143425

3. Haque M. E., Abe F., Mori M., Oyanagi A., Komatsu S., Kawaguchi K. Characterization of a wheat pathogenesis-related protein, TaBWPR-1.2, in seminal roots in response to waterlogging stress. Journal of Plant Physiology, 2014, vol. 171, no. 8, pp. 602–609. doi.org/10.1016/j.jplph.2013.12.003

4. Cawood M. E., Pretorius J. C., van der Westhuizen A. J., Pretorius Z. A. Disease development and PR-protein activity in wheat (Triticum aestivum) seedlings treated with plant extracts prior to leaf rust (Puccinia triticina) infection. Crop Protection, 2010, vol. 29, no. 11, pp. 1311–1319. doi.org/10.1016/j.cropro.2010.06.017

5. Simonetti E., Alba E., Delibes A. Сhromosomal location of four genes encoding Class III peroxidases in wheat. Phyton-International Journal of Experimental Botany, 2012, vol. 81, pp. 4–11.

6. Kuwabara C., Takezawa D., Shimada T., Hamada T., Fujikawa S., Arakawa K. Abscisic acid- and cold-induced thaumatin-like protein in winter wheat has an antifungal activity against snow mould, Microdochium nivale. Physiologia Plantarum, 2002, vol. 115, no. 1, pp. 101–110. doi.org/10.1034/j.1399-3054.2002.1150112.x

7. Valueva T. A., Mosolov V. V. Role of protease inhibitors in plant protection. Uspekhi biologicheskoi khimii [Biological Chemistry Reviews], 2002, vol. 42, pp. 193–216 (in Russian).

8. Schweizer P., Christoffel A., Dudler R. Transient expression of members of the germin-like gene family in epidermal cells of wheat confers disease resistance. The Plant Journal, 1999, vol. 20, no. 5, pp. 541–552. doi.org/10.1046/j.1365-313x. 1999.00624.x

9. Filipenko E. A., Kochetov A. V., Kanayama Y., Malinovsky V. I., Shumny V. K., Association between PR proteins with ribonuclease activity and plant resistance against pathogenic fungi. Vavilovskii zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breeding, 2013, vol. 17, no. 2. pp. 326–334 (in Russian).

10. Selitrennikof C. Antifungal Proteins. Applied and Environmental Microbiology, 2001, vol. 67, no. 7, pp. 2883–2894. doi.org/10.1128/aem.67.7.2883-2894.2001

11. Roberts W., Selitrennikoff C. P. Zeamatin, an antifungal protein from maize with membrane-permeabilizing activity. Journal of General Microbiology, 1990, vol. 136, no. 9, pp. 1771–1778. doi.org/10.1099/00221287-136-9-1771

12. Shestibratov K. A., Dolgov S. V. Transgenic strawberry plants expressing a thaumatin II gene demonstrate enhanced resistance to Botrytis cinerea. Scientia Horticulturae, 2005, vol. 106, no. 2, pp. 177–189. doi.org/10.1016/j.scienta.2005.03.016

13. Xing L.-P., Wang H.-Z., Jiang Z.-N., Ni J.-L., Cao A.-Z., Yu L., Chen P.-D. Transformation of wheat thaumatin-like protein gene and analysis of reactions to powdery mildew and fusarium head blight in transgenic plants. Acta Agronomica Sinica, 2008, vol. 34, no. 3, pp. 349–354. doi.org/10.1016/s1875-2780(08)60014-0

14. Mackintosh C. A., Lewis J., Radmer L. E., Shin S., Heinen S. J., Smith L. A., Wyckoff M. N., Dill-Macky R., Evans C. K., Kravchenko S., Baldridge G. D., Zeyen R. J., Muehlbauer G. J. Overexpression of defense response genes in transgenic wheat enhances resistance to Fusarium head blight. Plant Cell Reports, 2007, vol. 26, pp. 479–488. doi.org/10.1007/s00299-006- 0265-8

15. Radyuk M. S., Domanskaya I. N., Budakova E. A., Dremuk I. A., Shalygo N. V. Effect of abiotic environmental factors on the content of antimicrobial proteins thionins in barley (Hordeum vulgare). Vestsi Natsyyanal’nai akademii navuk Belarusi. Seryya biyalagichnych navuk = Proceedings of the National Academy of Sciences of Belarus. Biological series, 2014, no. 4, pp. 50–53 (in Russian).


Review

Views: 778


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 1561-8323 (Print)
ISSN 2524-2431 (Online)