Preview

Doklady of the National Academy of Sciences of Belarus

Advanced search

ANALYSIS OF GYNZENOSIDES IN THE ROOTS OF PANAX GINSENG INTRODUCED IN THE CENTRAL BOTANICAL GARDEN OF NAS OF BELARUS

https://doi.org/10.29235/1561-8323-2018-62-4-447-454

Abstract

For the first time, a detailed study of the qualitative and quantitative composition of ginsenosides in the Panax ginseng roots was carried out with the help of high-performance liquid chromatography combined with mass spectrometry (HPLC-MS). The plants were introduced into the conditions of the Republic of Belarus at the experimental plot of the Central Botanical Garden of the National Academy of Sciences of Belarus. It was found that in the examined roots, all basic neutral glycosides of ginseng (ginsenosides Rb1, Rc, Rb2/Rb3, Rd, Rf, Rg1 and Re), as well as their malonylated derivatives (malonylginsenosides Rb1, Rc, Rb2/Rb3, Rd, Rg1 and Re) and some “minor” ginsenosides (20-gluco-ginsenoside Rf, notoginsenosides R1 and R2, isomers of malonyl-ginsenosides Rb1 and Rd) are present. The research also showed that different parts of the P. ginseng roots differ significantly in a total content of ginsenosides: for the main root, this parameter was 3.3 % of dry mass, and for the lateral roots – 7.8 % of dry mass.

About the Authors

Dmitry V. Kochkin
Lomonosov Moscow State University
Russian Federation

Kochkin Dmitry Vladimirovich – Ph. D. (Biology), Senior researcher

1/12, Leninskie gory, 119234, Moscow



Elena S. Glagoleva
Lomonosov Moscow State University
Russian Federation

Glagoleva Elena Sergeevna – Researcher

1/12, Leninskie gory, 119234, Moscow



Boris A. Galischev
Ural Federal University named after the first President of Russia B. N. Yeltsin
Russian Federation

Galishev Boris Aleksandrovich – Engineer-researcher

48, Kuibyshev Str., 620083, Yekaterinburg



Elena V. Spiridovich
Central Botanical Garden of the National Academy of Sciences of Belarus
Belarus

Spiridovich Elena Vladimirovna – Ph. D. (Biology), Assistant Professor, Head of the Laboratory

2B, Surganov Str., 220012, Minsk



Alexander M. Nosov
Lomonosov Moscow State University
Russian Federation

Nosov Alexander Mikhailovich – D. Sc. (Biology), Professor, Head of the Department

1/12, Leninskie gory, 119234, Moscow



Vladimir N. Reshetnikov
Central Botanical Garden of the National Academy of Sciences of Belarus
Belarus

Reshetnikov Vladimir Nikolaevich – Academician, D. Sc. (Biology), Professor, Head of the Department

2B, Surganov Str., 220012, Minsk



References

1. Shin B.-K., Kwon S. W., Park J. H. Chemical diversity of ginseng saponins from Panax ginseng. Journal of Ginseng Research, 2015, vol. 39, no. 4, pp. 287–298. https://doi.org/10.1016/j.jgr.2014.12.005

2. Court W. E. The Principal Active Chemicals in Panax Species. Ginseng, the Genus Panax. London, CRC Press, 2000, pp. 55–116.

3. Christensen L. P. Ginsenosides: chemistry, biosynthesis, analysis, and potential health effects. Advances in Food and Nutrition Research, 2008, vol. 55, pp. 1–99. https://doi.org/10.1016/s1043-4526(08)00401-4

4. Qi L. W., Wang C. Z., Yuan C. S. Ginsenosides from American ginseng: chemical and pharmacological diversity. Phytochemistry, 2011, vol. 72, no. 8, pp. 689–699. https://doi.org/10.1016/j.phytochem.2011.02.012

5. Kochkin D. V., Galishev B. A., Glagoleva E. S., Titova M. V., Nosov A. M. Rare triterpene glycoside of ginseng (ginsenoside malonyl-Rg1) detected in plant cell suspension culture of Panax japonicus var. repens. Russian Journal of Plant Physiology, 2017, vol. 64, no. 5, pp. 649–656. https://doi.org/10.1134/s102144371705003x

6. Li F., Lv C., Li Q., Wang J., Song D., Liu P., Zhang D., Lu J. Chemical and bioactive comparison of flowers of Panax ginseng Meyer, Panax quinquefolius L., and Panax notoginseng Burk. Journal of Ginseng Research, 2017, vol. 41, no. 5, pp. 487–495. https://doi.org/10.1016/j.jgr.2016.08.008

7. Stavrianidi A., Rodin I., Braun A., Stekolshchikova E., Shpigun O. Single-run HPLC/ESI-LITMS profiling of ginsenosides in plant extracts and ginseng based products. Biomedical Chromatography, 2015, vol. 29, no. 6, pp. 853–859. https:// doi.org/10.1002/bmc.3364

8. Sun T.-T., Liang X.-L., Zhu H.-Y., Peng X.-L., Guo X.-J., Zhao L.-S. Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography–electron spray ionization–MS/MS. Journal of Ginseng Research, 2016, vol. 40, no. 3, pp. 220–228. https://doi.org/10.1016/j.jgr.2015.07.008

9. Wang Y.-S., Jin Y.-P., Gao W., Xiao S.-Y., Zhang Y.-W., Zheng P.-H., Wang J., Liu J.-X., Sun C.-H., Wang Y.-P. Complete (1)H-NMR and (13)C-NMR spectral assignment of five malonyl ginsenosides from the fresh flower buds of Panax ginseng. Journal of Ginseng Research, 2016, vol. 40, no. 3, pp. 245–250. https://doi.org/10.1016/j.jgr.2015.08.003

10. Qiu S., Yang W. Z., Yao C. L., Shi X. J., Li J. Y., Lou Y., Duan Y. N., Wu W. Y., Guo D. A. Malonylginsenosides with Potential Antidiabetic Activities from the Flower Buds of Panax ginseng. Journal of Natural Products, 2017, vol. 80, no. 4, pp. 899–908. https://doi.org/10.1021/acs.jnatprod.6b00789

11. Chen Y., Zhao Z., Chen H., Brand E., Yi T., Qin M., Liang Z. Determination of ginsenosides in Asian and American ginsengs by liquid chromatography–quadrupole/time-of-flight MS: assessing variations based on morphological characteristics. Journal of Ginseng Research, 2017, vol. 41, no. 1, pp. 10–22. https://doi.org/10.1016/j.jgr.2015.12.004

12. Liu Z., Li Y., Li X., Ruan C.-C., Wang L.-J., Sun G.-Z. The effects of dynamic changes of malonyl ginsenosides on evaluation and quality control of Panax ginseng C. A. Meyer. Journal of Pharmaceutical and Biomedical Analysis, 2012, vol. 64–65, pp. 56–63. https://doi.org/10.1016/j.jpba.2012.02.005

13. Du X. W., Wills R. B. H., Stuart D. L. Changes in neutral and malonyl ginsenosides in American ginseng (Panax quinquefolium) during drying, storage and ethanolic extraction. Food Chemistry, 2004, vol. 86, no. 2, pp. 155–159. https://doi. org/10.1016/j.foodchem.2003.11.003


Review

Views: 1604


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 1561-8323 (Print)
ISSN 2524-2431 (Online)