IDENTIFICATION OF GENES ENCODING STRESS-ASSOCIATED PROTEINS CONTAINING THE DOMAINS A20/AN1 IN THE APPLE GENOME IN SILICO AND ANALYSIS OF THEIR PHYLOGENETIC RELATIONSHIP
https://doi.org/10.29235/1561-8323-2018-62-4-455-462
Abstract
Stress-associated proteins (SAPs) are the transcription factors containing the zinc-finger domains A20 and/or AN1. Genome-wide identification and characterization of an apple variety of Golden Delicious stress-associated proteins was performed, potential SAPs were found. Each of them contains, at least, one zinc-finger domain AN1. A phylogenetic tree was constructed on the basis of the amino acid sequences of apple SAP and homologues from other species (dicotyledonous cotton and monocotyledonous rice) to assess their phylogenetic relationships. The sequences ahead of the translation initiation site of SAPs genes were analyzed to discover cis-elements involved in the gene expression regulation. The analysis shows that apple SAPs are involved in a complex network of regulatory elements that controls the vitality of plant cells. It can be assumed that the expression of SAPs in apple trees occurs constantly, but if conditions of a plant growth are changed, their level increases. Regulation of the SAP expression may depend on a plant ontogenesis stage and it may vary in different tissues.
About the Authors
Polina V. Kuzmitskaya
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Kuzmitskaya Polina Viktorovna – Researcher
27, Akademicheskaya Str., 220072, Minsk
Oksana Yu. Urbanovich
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Urbanovich Oksana Yur’evna – D. Sc. (Biology), Нead of the Laboratory
27, Akademicheskaya Str., 220072, Minsk
Aleksandr V. Kilchevsky
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Kilchevsky Aleksandr Vladimirovich – Academician, D. Sc. (Biology), Professor, Head of the Laboratory
27, Akademicheskaya Str., 220072, Minsk
References
1. Dixit V. M., Green S., Sarma V., Holzman L. B., Wolf F. W., O’Rourke K., Ward P. A., Prochownik E. V., Marks R. M., Tumor necrosis factor-alpha induction of novel gene products in human endothelial cells including a macrophage-specific chemotaxin. Journal of Biological Chemistry, 1990, vol. 265, no. 5, pp. 2973–2978.
2. Opipari A. W., Jr., Boguski M. S., Dixit V. M. The A20 cDNA induced by tumor necrosis factor alpha encodes a novel type of zinc finger protein. Journal of Biological Chemistry, 1990, vol. 265, no. 25, pp. 14705–14708.
3. Rebagliati M. R., Weeks D. L., Harvey R. P., Melton D. A. Identification and cloning of localized maternal RNAs from Xenopus eggs. Cell, 1985, vol. 42, no. 3, pp. 769–777. https://doi.org/10.1016/0092-8674(85)90273-9
4. Huang J., Teng L., Li L., Liu T., Li L., Chen D., Xu L.-G., Zhai Z., Shu H.-B. ZNF216 Is an A20-like and IκB Kinase γ-Interacting Inhibitor of NFκB Activation. Journal of Biological Chemistry, 2004, vol. 279, no. 16, pp. 16847–16853. https:// doi.org/10.1074/jbc.m309491200
5. Mukhopadhyay A., Vij S., Tyagi A. K. Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco. Proceedings of the National Academy of Sciences, 2004, vol. 101, no. 16, pp. 6309–6314. https://doi.org/10.1073/pnas.0401572101
6. Stroher E., Wang X. J., Roloff N., Klein P., Husemann A., Dietz K. J. Redox-dependent regulation of the stress-induced zinc-finger protein SAP12 in Arabidopsis thaliana. Molecular Plant, 2009, vol. 2, no. 2, pp. 357–367. https://doi.org/10.1093/ mp/ssn084
7. Zhang Y., Lan H., Shao Q., Wang R., Chen H., Tang H., Zhang H., Huang J. An A20/AN1-type zinc finger protein modulates gibberellins and abscisic acid contents and increases sensitivity to abiotic stress in rice (Oryza sativa). Journal of Experimental Botany, 2016, vol. 67, no. 1, pp. 315–326. https://doi.org/10.1093/jxb/erv464
8. Tyagi H., Jha S., Sharma M., Giri J., Tyagi A. K. Rice SAPs are responsive to multiple biotic stresses and overexpression of OsSAP1, an A20/AN1 zinc-finger protein, enhances the basal resistance against pathogen infection in tobacco. Plant Science, 2014, vol. 225, pp. 68–76. https://doi.org/10.1016/j.plantsci.2014.05.016
9. Vij S., Tyagi A. K., Genome-wide analysis of the stress associated protein (SAP) gene family containing A20/AN1 zinc-finger(s) in rice and their phylogenetic relationship with Arabidopsis. Molecular Genetics and Genomics, 2006, vol. 276, no. 6, pp. 565–575. https://doi.org/10.1007/s00438-006-0165-1
10. Gao W., Long L., Tian X., Jin J., Liu H., Zhang H., Xu F., Song C. Genome-wide identification and expression analysis of stress-associated proteins (SAPs) containing A20/AN1 zinc finger in cotton. Molecular Genetics and Genomics, 2016, vol. 291, no. 6, pp. 2199–2213. https://doi.org/10.1007/s00438-016-1252-6
11. Gonzalez D. H., ed. Plant Transcription Factors. Boston, Academic Press, 2016. 422 p. https://doi.org/10.1016/c2013- 0-19051-4
12. Wegner M. All purpose Sox: The many roles of Sox proteins in gene expression. International Journal of Biochemistry & Cell Biology, 2010, vol. 42, no. 3, pp. 381–390. https://doi.org/10.1016/j.biocel.2009.07.006
13. Solanke A., Sharma M., Tyagi A. K., Sharma A. Characterization and phylogenetic analysis of environmental stressresponsive SAP gene family encoding A20/AN1 zinc finger proteins in tomato. Molecular Genetics and Genomics, 2009, vol. 282, no. 2, pp. 153–164. https://doi.org/10.1007/s00438-009-0455-5
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