Polyvinyl alcohol magnetic microspheres containing 2-aminopyrimidin-bis(ethene)bis(dialkylphenylamine) compounds as fluorescent pH indicators
https://doi.org/10.29235/1561-8323-2018-62-5-563-568
Abstract
The fluorescent dyes 4,4′-(1E,1′Е)-2,2′-(2-aminopyrimidin-4,6-diyl) bis(ethene-2,l-diyl) bis(N,N-dimethylphenylamine) (АРМ) and 4′-(1E,1′Е)-2,2′-(2-aminopyrimidin-4,6-diyl) bis(ethene-2,l-diyl) bis(N,N-diethylphenylamine) (APE) were incorporated into glutaraldehyde-crosslinked polyvinyl alcohol microspheres containing magnetite. Fluorescence spectra of the obtained microspheres depend on pH of the medium. A technique for pH measurement within the range of 4–9 based on calculation of the green/red ratio of the fragments of color microimages of the microspheres was developed. only negligible loss of the fluorochromes from the microspheres occured during the storage time of 180 days. Thus the microspheres may be used as local pH indicators, e. g. for phagocytosis studies.
About the Authors
K. V. Laznev
Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus
Belarus
Belarus
Laznev Konstantin Vladimirovich – Researcher.
36, F. Skorina Str., 220141, Minsk
Zh. V. Ignatovich
Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus
Belarus
Belarus
Ignatovich Zhanna Vladimirovna – Ph. D. (Chemistry), leading researcher.
36, F. Skorina Str., 220141, Minsk
I. N. Kukhta
Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus
Belarus
Belarus
Kukhta Irina Nikolaevna – Junior researcher.
36, F. Skorina Str., 220141, Minsk
A. V. Agabekov
Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus
Belarus
Belarus
Agabekov Vladimir Yenokovich – Academician, D. Sc. (Chemistry), Professor, Director.
36, F. Skorina Str., 220141, Minsk
References
1. Bradley M., Alexander L., Duncan K., Chennaoui M., Jones A.C., Sanchez-Martm R. M. pH sensing in living cells using fluorescent microspheres. Bioorganic & Medicinal Chemistry Letters, 2008, vol. 18, no. 1, pp. 313-317. https://doi.org/10.1016/j.bmcl.2007.10.075
2. Vergne I., Constant P., Laneelle G. Phagosomal pH determination by dual fluorescence flow cytometry. Analytical Biochemistry, 1998, vol. 255, no. 1, pp. 127-132. https://doi.org/10.1006/abio.1997.2466
3. Laznev K. V., Agabekov V. E. Binding of magnetic gel microspheres to blood mononuclear cells. Doklady Natsional ’noi akademii nauk Belarusi = Doklady of the National Academy of Sciences of Belarus, 2016, vol. 60, no. 2, pp. 67-72 (in Russian).
4. Yermolenko E. M., Ibragimova Z. A., Khodosovskaya E. V., Semerikhina S. E., Kolesnikova T. S., Laznev K. V., Zhav-nerko G. K., Kashevskiy B. E., Agabekov V. E. Hydrogel magnetic microspheres: usage for immunomagnetic cell separation. BGMU: 90 let v avangarde medicinskoi nauki i praktiki [BSMU: 90 years in the forefront of medical science and practice], Minsk, 2012, iss. 2, pp. 89-93 (in Russian).
5. Bo Liu, Xue-Lei Hu, Jun Liu, Yuan-Di Zhao, Zhen-Li Huang. Synthesis and photophysical properties of novel pyrimidine-based two-photon absorption chromophores. Tetrahedron Letters, 2007, vol. 48, no. 34, pp. 5958-5962. https://doi.org/10.1016/j.tetlet.2007.06.122
6. Frisch M. J., ed. Gaussian 03, Revision D 01. Gaussian, Inc., Wallingford CT, 2004.
7. ImageJ: Image processing and analysis in Java (2018). Available at: https://imagej.nih.gov/ij (accessed 10 May 2018).
8. Fei Wu, Zhihua Zhou, Jing Su, Liangming Wei, Weien Yuan, Tuo Jin. Development of dextran nanoparticles for stabilizing delicate proteins. Nanoscale Research Letters, 2013, vol. 8, no. 1, pp. 197-204. https://doi.org/10.1186/1556-276x-8-197
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