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Grain quality in bread wheat lines T. aestivum with introgression of genetic material T. dicoccoides and T. dicoccum

https://doi.org/10.29235/1561-8323-2018-62-6-712-718

Abstract

Related wild and cultural wheat species are regularly involved for expanding T. aestivum genetic diversity because they contain many valuable genes. We evaluated the effect of the genetic material of tetraploid species of the genus Triticum (T. dicoccoides, T. dicoccum) on the grain quality of introgression lines of spring bread wheat. The composition of the high molecular weight glutenin subunits which play an essential role in the formation of wheat baking properties was identified in the introgression lines of bread wheat and their parental forms. The traits of grain quality (hardness, protein and gluten content, gluten quality) were also evaluated. The lines with Glu-1 loci alleles from wheat relatives T. dicoccoides and Т. dicoccum were selected. It was found that the introgression of alien genetic material into the common wheat genome had a positive effect on the parameters of grain quality such as hardness, protein and gluten content. The lines with Glu-A1 loci alleles from T. dicoccoides and Glu-B1 from T. dicoccum were at the level of a parent wheat variety or of a higher gluten quality. As a result of the research, the new lines of bread soft wheat with high grain quality were found and can be used in the crop breeding.

About the Authors

O. A. Orlovskaya
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Minsk
Belarus
Ph. D. (Biology), Leading researcher


S. I. Vakula
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Minsk
Belarus
Ph. D. (Biology). Researcher


L. V. Khotyleva
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Minsk
Belarus
Academician, D. Sc. (Biology), Professor. Chief researcher


A. V. Kilchevsky
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Minsk
Belarus
Academician, D. Sc. (Biology), Professor, Chief scientific secretary


References

1. Pshenichnikova T. A., Ermakova M. F., Chistyakova A. k., Shchukina l. V., lapochkina I. o. Technological properties of grain and flour in soft wheat lines with introgression from Aegilops speltoides Tausch. Sel’skokhozyaistvennaya biologiya = Agricultural Biology, 2007, vol. 42, no. 5, pp. 86–89 (in Russian).

2. Sibikeev S. N., krupnova o. V., Voronina S. A., krupnov V. A., Druzhin A. E. Effects of an lr26 translocation on grain productivity and grain protein content in spring bread wheat. Annual Wheat Newsletter. USA, 2009, vol. 55, pp. 176.

3. Niu Z. X., klindworth R., Wang R. R.-C., Jauhar P. P., larkin P. J., Xu S. S. Characterization of HMW glutenin subunits in Thinopyrum intermedium, Th. bessarabicum, Lophopyrum elongatum, Aegilops markgrafii, and their addition lines in wheat. Crop Science, 2011, vol. 51, no. 2, pp. 667–677. https://doi.org/10.2135/cropsci2010.04.0235

4. Zhang D., yuan y., Su y., li S. Analysis of dough rheological property and gluten quality characteristics in wild emmer wheat (Triticum dicoccoides (körn. ex Asch. et Graebn.) Schweinf.). Genetic Resources and Crop Evolution, 2016, vol. 63, no. 4, pp. 675–683. https://doi.org/10.1007/s10722-015-0275-x

5. Morgun V. V., Sichkar S. M., Pochinok V. M., Ninieva A. k., Chugunkova T. V. Characterization of spelt collection samples (Triticum spelta l.) by elements of plant productivity structure and baking quality. Fiziologiya rasteniy i genetika = Plant Physiology and Genetics, 2016, vol. 48, no 2, pp. 112–119 (in Ukrainian).

6. Singh N. k., Shepherd k. W., Cornish G. B. A simplified SDS-PAGE procedure for separating lMW subunits of glutenin. Journal of Cereal Science, 1991, vol. 14, no. 3, pp. 203–208. https://doi.org/10.1016/s0733-5210(09)80039-8

7. laemmli U. k. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, vol. 227, no. 5259, pp. 680–685. https://doi.org/10.1038/227680a0

8. Payne P. I., lawrence G. J. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Research Communication, 1983, vol. 11, no. 1, pp. 29–35.

9. levy A. A., Feldman M. Increase in grain protein percentage in high-yielding common wheat breeding lines by genes from wild tetraploid wheat. Euphytica, 1987, vol. 36, no. 2, pp. 353–359. https://doi.org/10.1007/bf00041478

10. kade M. A., Barneix J., olmos S., Dubcovsky J. Nitrogen uptake and remobilization in tetraploid langdon durum wheat and a recombinant substitution line with the high grain protein gene Gpc-B1. Plant Breeding, 2005, vol. 124, no. 4, pp. 343–349. https://doi.org/10.1111/j.1439-0523.2005.01110.x

11. Pshenichnikova T. A., Ermakova M. F., Chistyakova A. k. Shchukina l. V., Berezovskaya E. V., lochwasser U., Röder M., Börner A. Mapping of the quantitative trait loci (QTl) associated with grain quality characteristics of the bread wheat grown under different environmental conditions. Russian Journal of Genetics, 2008, vol. 44, no. 1, pp. 74–84. https:// doi.org/10.1134/s1022795408010109

12. Ribeiro M., Nunes-Miranda J. D., Branlard G., Carrillo J. M., Rodriguez-Quijano M., Igrejas G. one hundred years of grain omics: identifying the glutens that feed the world. Journal of Proteome Research, 2013, vol. 12, no. 11, pp. 4702– 4716. https://doi.org/10.1021/pr400663t

13. Vincentini o., Maialetti F., Gazza l., Silano M., Dessì M., De Vincenzi M., Pogna N. E. Environmental factors of celiac disease: cytotoxicity of hulled wheat species Т. топососсит, T. turgidum l. ssp dicoccum and T. aestivum ssp. spelta. Journal of Gastroenterology and Hepatology, 2007, vol. 22, no. 11, pp. 1816–1822. https://doi.org/10.1111/j.1440- 1746.2006.04680.x

14. Margiotta B., Colaprico G., Urbano M. Polymorphism of high Mr glutenin subunits in wild emmer Triticum turgidum subsp. dicoccoides: chromatographic, electrophoretic separation and PCR analysis of their encoding genes. Genetic Resources and Crop Evolution, 2014, vol. 61, no. 2, pp. 331–343. https://doi.org/10.1007/s10722-013-0037-6

15. Rasheed A., Xia X., yan yu., Appels R., Mahmood T., He Z. Wheat seed storage proteins: advances in molecular genetics, diversity and breeding applications. Journal of Cereal Science, 2014, vol. 60, no. 1, pp. 11–24. https://doi.org/10.1016/j. jcs.2014.01.020


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ISSN 1561-8323 (Print)
ISSN 2524-2431 (Online)