Sugar-dependent regulation of the phospholipase D activity in oat seedlings
https://doi.org/10.29235/1561-8323-2019-63-1-61-71
Abstract
The dependence of the phospholipase D (PLD) activity, catalyzing the catabolism of glycerophospholipids, on exogenous soluble sugars under the conditions of sugar starvation caused by darkening of plants was studied in oat (Avena sativa L.) seedlings. It was found that the PLD activity in etiolated oat seedlings was suppressed by the treatment of plants with mono- and disaccharides: glucose, sucrose and mannose. These sugars neutralized the activating effect of darkening on the activity of PLD in green oat seedlings. The treatment of oat seedlings with inorganic phosphate also led to the modulation of the PLD activity similar to that with sugars. In this case, the action of the tested sugars mimicked the light modulation of the PLD activity. Sequencing the partial mRNA sequence of oat PLD gene showed more than 80 % homology with the sequences PlDαof other cereal species. Illumination of etiolated oat seedlings with red light and darkening of green seedlings in darkness inhibited or induced the PLD expression, respectively. Thus, the expression of PLD and the enzymatic activity of PLD showed the sensitivity to changes in the light conditions of a plant growth. Consequently, these data indicate that PLD plays an important role in adaptation of oat plants to darkening and sugar starvation.
About the Authors
A. M. Каbachevskaya
Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus
Belarus
Belarus
Kabachevskaya Alena Mikhaylovna - Ph. D. (Biology), Senior researcher.
27, Ac-ademicheskaya Str., 220072, Minsk
S. V. Sukhaveyeva
Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus
Belarus
Belarus
Sukhaveyeva Sviatlana Vladimirovna - Junior researcher.
27, Academicheskaya Str., 220072, Minsk
I. D. Volotovski
Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus
Belarus
Belarus
Volotovski Igor Dmitrievich - Academician, D. Sc. (Biology), Professor, Head of the Laboratory.
27, Academicheskaya Str., 220072, Minsk
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