Polymorphism of microsatellite loci in silver (Hypophthalmichthys molitrix Val.) and bighead (Hypophthalmichthys nobilis Rich.) carps grown in aquaculture in the Republic of Belarus

In this study, we evaluated the genetic diversity of silver (Hypophthalmichthys molitrix Val.) and bighead (Hypophthalmichthys nobilis Rich.) carps grown in aquaculture in the territory of the Republic of Belarus. Genotyping was obtained for 11 STR-loci - Hmo11, Hmo13, Hmo15, Hmo25, Hmo26, Hmo31, Hmo33, Hmo 34, Hmo36, Hmo37, and Hmo40. The following parameters were calculated: the average number of alleles per locus, the effective number of alleles, the levels of expected and observed heterozygosity, the value of the Shannon information index and the fixation indexes F_IS and F_ST. The obtained results indicate a moderate (for silver carp) and sufficiently high (for bighead carp) genetic diversity of the studied samples of carps up to the possibility to allocate several groups for subsequent work on obtaining linear material and further reproduction, including commercial fish. However, to achieve these goals, it is necessary to pay special attention to the selection of pairs of producers taking into account the results of molecular genetic analysis. The scheme for the differentiation of hybrid individuals between silver and bighead carps and the use of molecular genetic analysis of STR-loci would be proposed. This approach can be used as a minimally invasive rapid test in breeding and reproductive programs for the studied fish species.

Communicated by Academician A. V. Kilchevsky

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